Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

2017 ◽  
pp. 47-54 ◽  
Author(s):  
Daichi Susaki ◽  
Daisuke Maruyama ◽  
Ramesh Yelagandula ◽  
Frederic Berger ◽  
Tomokazu Kawashima
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Actin Dynamics

An aggregation-induced phosphorescence probe for calcium ion-specific detection and live-cell imaging in Arabidopsis thaliana

2019 ◽  
Vol 55 (33) ◽  
pp. 4841-4844 ◽  
Author(s):  
Guilin Chen ◽  
Zaicai Zhou ◽  
Hui Feng ◽  
Chenyan Zhang ◽  
Yifan Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Calcium Ion ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Probe ◽  
Specific Detection ◽  
Phosphorescence Probe

A molecular probe with aggregation-induced phosphorescence (AIP) properties for calcium ion-specific detection and imaging in vivo was designed.

scholarly journals F-Actin Dynamics in Neurospora crassa

2010 ◽  
Vol 9 (4) ◽  
pp. 547-557 ◽  
Author(s):  
Adokiye Berepiki ◽  
Alexander Lichius ◽  
Jun-Ya Shoji ◽  
Jens Tilsner ◽  
Nick D. Read
Keyword(s):  
Neurospora Crassa ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Actin Dynamics ◽  
Actin Binding ◽  
Content Type ◽  
Actin Cables ◽  
Germ Tubes

ABSTRACT This study demonstrates the utility of Lifeact for the investigation of actin dynamics in Neurospora crassa and also represents the first report of simultaneous live-cell imaging of the actin and microtubule cytoskeletons in filamentous fungi. Lifeact is a 17-amino-acid peptide derived from the nonessential Saccharomyces cerevisiae actin-binding protein Abp140p. Fused to green fluorescent protein (GFP) or red fluorescent protein (TagRFP), Lifeact allowed live-cell imaging of actin patches, cables, and rings in N. crassa without interfering with cellular functions. Actin cables and patches localized to sites of active growth during the establishment and maintenance of cell polarity in germ tubes and conidial anastomosis tubes (CATs). Recurrent phases of formation and retrograde movement of complex arrays of actin cables were observed at growing tips of germ tubes and CATs. Two populations of actin patches exhibiting slow and fast movement were distinguished, and rapid (1.2 μm/s) saltatory transport of patches along cables was observed. Actin cables accumulated and subsequently condensed into actin rings associated with septum formation. F-actin organization was markedly different in the tip regions of mature hyphae and in germ tubes. Only mature hyphae displayed a subapical collar of actin patches and a concentration of F-actin within the core of the Spitzenkörper. Coexpression of Lifeact-TagRFP and β-tubulin–GFP revealed distinct but interrelated localization patterns of F-actin and microtubules during the initiation and maintenance of tip growth.

scholarly journals Live-cell imaging of actin dynamics reveals mechanisms of stereocilia length regulation in the inner ear

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Meghan C. Drummond ◽  
Melanie Barzik ◽  
Jonathan E. Bird ◽  
Duan-Sun Zhang ◽  
Claude P. Lechene ◽  
...  
Keyword(s):  
Inner Ear ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Actin Dynamics ◽  
Length Regulation

scholarly journals Author response: Live cell imaging of meiosis in Arabidopsis thaliana

2019 ◽  
Author(s):  
Maria A Prusicki ◽  
Emma M Keizer ◽  
Rik P van Rosmalen ◽  
Shinichiro Komaki ◽  
Felix Seifert ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Author Response

scholarly journals Katanin Effects on Dynamics of Cortical Microtubules and Mitotic Arrays in Arabidopsis thaliana Revealed by Advanced Live-Cell Imaging

2017 ◽  
Vol 8 ◽  
Author(s):  
George Komis ◽  
Ivan Luptovčiak ◽  
Miroslav Ovečka ◽  
Despina Samakovli ◽  
Olga Šamajová ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cortical Microtubules

scholarly journals Live-Cell Imaging Reveals the Dynamics of Two Sperm Cells during Double Fertilization in Arabidopsis thaliana

2011 ◽  
Vol 21 (6) ◽  
pp. 497-502 ◽  
Author(s):  
Yuki Hamamura ◽  
Chieko Saito ◽  
Chie Awai ◽  
Daisuke Kurihara ◽  
Atsushi Miyawaki ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Sperm Cells ◽  
Double Fertilization
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