scholarly journals Ratiometric two-photon fluorescent probe for in situ imaging of carboxylesterase (CE)-mediated mitochondrial acidification during medication

2019 ◽  
Vol 55 (76) ◽  
pp. 11358-11361 ◽  
Author(s):  
Ao Jiang ◽  
Guang Chen ◽  
Jie Xu ◽  
Yuxia Liu ◽  
Guanghui Zhao ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepatoma Cells ◽  
Two Photon ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
In Situ Imaging

A probe for imaging of mitochondrial carboxylesterase and pH has been developed for the visualization of carboxylesterase-mediated acidification in hepatoma cells and hepatic tissues during the administration of antipyretic and anti-inflammatory drugs.

scholarly journals Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO–/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish

2019 ◽  
Vol 91 (3) ◽  
pp. 1719-1723 ◽  
Author(s):  
Yujing Zuo ◽  
Yu Zhang ◽  
Baoli Dong ◽  
Zhiming Gou ◽  
Tingxin Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cells ◽  
Binding Reaction ◽  
In Situ Imaging

In situ imaging of the mouse cochlea using two-photon microscopy

2013 ◽  
Author(s):  
Xin Yang ◽  
Ye Pu ◽  
Demetri Psaltis ◽  
Konstantina M. Stankovic
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon ◽  
In Situ Imaging

scholarly journals Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4274
Author(s):  
Gabrielle B. Novais ◽  
Stefane dos Santos ◽  
Robertta J. R. Santana ◽  
Rose N. P. Filho ◽  
John L. S. Cunha ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Hydroalcoholic Extract ◽  
Inflammatory Conditions ◽  
Cord Injury ◽  
Light Curing ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
New Formulation

Spinal Cord Injury (SCI) promotes a cascade of inflammatory events that are responsible for neuronal death and glial scar formation at the site of the injury, hindering tissue neuroregeneration. Among the main approaches for the treatment of SCI, the use of biomaterials, especially gelatin methacryloyl (GelMA), has been proposed because it is biocompatible, has excellent mechanical properties, favoring cell adhesion and proliferation. In addition, it can act as a carrier of anti-inflammatory drugs, preventing the formation of glial scars. The present work presents the development and in situ application of a light-curing formulation based on GelMA containing a natural extract rich in anti-inflammatory, antioxidant and neuroprotective substances (hydroalcoholic extract of red propolis—HERP) in an experimental model of SCI in rats. The formulations were prepared and characterized by time of UV exposition, FTIR, swelling and degradation. The hydrogels containing 1 mg/mL of HERP were obtained by the exposure to UV radiation of 2 μL of the formulation for 60 s. The locomotor evaluation of the animals was performed by the scale (BBB) and demonstrated that after 3 and 7 days of the injury, the GelMA-HERP group (BBB = 5 and 7) presented greater recovery compared to the GelMA group (BBB = 4 and 5). Regarding the inflammatory process, using histomorphological techniques, there was an inflammation reduction in the groups treated with GelMA and GelMA-HERP, with decreases of cavitation in the injury site. Therefore, it is possible to conclude that the use of GelMA and GelMA-HERP hydrogel formulations is a promising strategy for the treatment of SCI when applied in situ, as soon as possible after the injury, improving the clinical and inflammatory conditions of the treated animals.

In-Situ Imaging of Azoreductase Activity in the Acute and Chronic Ulcerative Colitis Mice by a Near-Infrared Fluorescent Probe

2019 ◽  
Vol 91 (16) ◽  
pp. 10901-10907 ◽  
Author(s):  
Yang Tian ◽  
Yongfei Li ◽  
Wen-Li Jiang ◽  
Dong-Ye Zhou ◽  
Junjie Fei ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Fluorescent Probe ◽  
Chronic Ulcerative Colitis ◽  
Near Infrared ◽  
Azoreductase Activity ◽  
In Situ Imaging

In situ visualization of peroxisomal peroxynitrite in the livers of mice with acute liver injury induced by carbon tetrachloride using a new two-photon fluorescent probe

2019 ◽  
Vol 55 (47) ◽  
pp. 6767-6770 ◽  
Author(s):  
Yongqing Zhou ◽  
Ping Li ◽  
Nannan Fan ◽  
Xin Wang ◽  
Xiaoning Liu ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Fluorescent Probe ◽  
Acute Liver Injury ◽  
Two Photon ◽  
In Situ Visualization

In situ visualization of peroxisomal peroxynitrite in the livers of mice with acute liver injury using a new two-photon fluorescent probe.

In-Situ Synthesis of Active Pharmaceutical Ingredients Which Content Pyrazole Skeleton

2020 ◽  
Vol 17 (10) ◽  
pp. 743-748
Author(s):  
Sultan Pathan ◽  
Anil Repale ◽  
Girdhar Pal
Keyword(s):  
Aldol Condensation ◽  
Active Pharmaceutical Ingredients ◽  
Reaction Sequence ◽  
One Pot ◽  
Pharmaceutical Ingredients ◽  
One Pot Synthesis ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Work Up

Celecoxib containing pyrazole derivatives were synthesized by path aldol condensation of substituted ketone with trifluoroethyl acetate subsequently by cyclization of the formed chalcones with 4-methanesulfonylphenylhydrazine. Here, a one-pot synthesis of celecoxib and substituted analogues have been reported which are nonsteroidal anti-inflammatory drugs for their cyclooxygenase (COX) inhibition, anti-inflammatory activity and ulcerogenic liability. In order to intermediate work-up, a continuous one-pot synthesis has been developed, performing the entire reaction sequence that results in a shorter time with good yield.

A pH-Reversible Fluorescent Probe for in Situ Imaging of Extracellular Vesicles and Their Secretion from Living Cells

Nano Letters ◽  
2021 ◽  
Author(s):  
Hanzhuang Liu ◽  
Shaorui Liu ◽  
Yu Xiao ◽  
Wenting Song ◽  
Huize Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Extracellular Vesicles ◽  
Living Cells ◽  
In Situ Imaging

Two-photon Fluorogenic Probe Visualizing PGP-1 Activity in Inflammatory Tissue and Serum from Patients

2021 ◽  
Author(s):  
Hua Bai ◽  
Bin Fang ◽  
Xu-Jie Wang ◽  
Wenjing Qin ◽  
Yuhe Chen ◽  
...  
Keyword(s):  
Two Photon ◽  
Human Samples ◽  
Fluorogenic Probe ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Inflammatory Tissue

Pyroglutamate Aminopeptidase 1 (PGP-1) changes and distribution in human samples during inflammation are poorly characterized, limiting the understanding of the exact roles of PGP-1 in the metabolism of anti-inflammatory drugs....

Highly Sensitive Naphthalene-Based Two-Photon Fluorescent Probe for in Situ Real-Time Bioimaging of Ultratrace Cyclooxygenase-2 in Living Biosystems

2014 ◽  
Vol 86 (18) ◽  
pp. 9131-9138 ◽  
Author(s):  
Hua Zhang ◽  
Jiangli Fan ◽  
Kui Wang ◽  
Jing Li ◽  
Caixia Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Cyclooxygenase 2 ◽  
Two Photon ◽  
Highly Sensitive
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