Single-molecule determination of chemical equilibrium of DNA intercalation by electrical conductance

2021 ◽  
Author(s):  
Lu Zhang ◽  
Satoshi Kaneko ◽  
Shintaro Fujii ◽  
Manabu Kiguchi ◽  
Tomoaki Nishino
Keyword(s):  
Single Molecule ◽  
Chemical Equilibrium ◽  
Electrical Conductance ◽  
Dna Intercalation ◽  
Molecule Reaction ◽  
Association Reaction

We investigated a single-molecule reaction of DNA intercalation as an example of a bimolecular association reaction. Single-molecule conductance values of the product and reactant molecules adsorbed on an Au surface...

Determination of the size of lipid rafts studied through single-molecule FRET simulations

2021 ◽  
Author(s):  
Pablo Luis Hernández-Adame ◽  
Ulises Meza ◽  
Aldo A. Rodríguez-Menchaca ◽  
Sergio Sánchez-Armass ◽  
Jaime Ruiz-García ◽  
...  
Keyword(s):  
Lipid Rafts ◽  
Single Molecule ◽  
Single Molecule Fret

scholarly journals Single–motor mechanics and models of the myosin motor

2000 ◽  
Vol 355 (1396) ◽  
pp. 441-447 ◽  
Author(s):  
T. Yanagida ◽  
S. Esaki ◽  
A. Hikikoshi Iwane ◽  
Y. Inoue ◽  
A. Ishijima ◽  
...  
Keyword(s):  
Single Molecule ◽  
Atp Hydrolysis ◽  
Accurate Determination ◽  
Step Size ◽  
Single Molecule Level ◽  
Detection Techniques ◽  
Myosin Motor ◽  
Mechanochemical Coupling ◽  
Chemical Events

Recent progress in single–molecule detection techniques is remarkable. These techniques have allowed the accurate determination of myosin–head–induced displacements and how mechanical cycles are coupled to ATP hydrolysis, by measuring individual mechanical events and chemical events of actomyosin directly at the single–molecule level. Here we review our recent work in which we have made detailed measurements of myosin step size and mechanochemical coupling, and propose a model of the myosin motor.

Determination of the limits of applicability of the chemical equilibrium model to the detonation products of gas mixtures

2010 ◽  
Vol 4 (6) ◽  
pp. 969-976 ◽  
Author(s):  
U. F. Bryakina ◽  
S. A. Gubin ◽  
A. M. Tereza ◽  
V. A. Shargatov
Keyword(s):  
Chemical Equilibrium ◽  
Equilibrium Model ◽  
Gas Mixtures ◽  
Detonation Products ◽  
Chemical Equilibrium Model

Single-molecule avidin–biotin association reaction studied by force-clamp spectroscopy

2008 ◽  
Vol 108 (10) ◽  
pp. 1135-1139 ◽  
Author(s):  
Mélanie Favre ◽  
Serguei K. Sekatskii ◽  
Giovanni Dietler
Keyword(s):  
Single Molecule ◽  
Association Reaction ◽  
Force Clamp

Rapid method for the determination of electrical conductance of ion-exchange membranes

1968 ◽  
Vol 72 (12) ◽  
pp. 4314-4316 ◽  
Author(s):  
Vaidyanathaiyer Subrahmanyan ◽  
N. Lakshminarayanaiah
Keyword(s):  
Ion Exchange ◽  
Rapid Method ◽  
Electrical Conductance ◽  
Ion Exchange Membranes

scholarly journals High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

2020 ◽  
Author(s):  
Mike Filius ◽  
Sung Hyun Kim ◽  
Ivo Severins ◽  
Chirlmin Joo
Keyword(s):  
Structural Analysis ◽  
High Resolution ◽  
Single Molecule ◽  
Single Object ◽  
Single Molecule Fret ◽  
Dna Strands ◽  
Versatile Tool ◽  
Fret Efficiency ◽  
Fret Pair

ABSTRACTSingle-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object. The limited number of available FRET pair fluorophores and complicated data analysis makes it challenging to apply single-molecule FRET for structural analysis of biomolecules. Currently, only a couple of FRET pairs can be reliably measured on a single object. Here we present an approach that allows for the determination of multiple distances between FRET pairs in a single object. We use programmable, transient binding between short DNA strands to resolve the FRET efficiency of multiple fluorophore pairs. By allowing only a single FRET pair to be formed at a time, we can determine the FRET efficiency and pair distance with sub-nanometer resolution. We determine the distance between other pairs by sequentially exchanging DNA strands. We name this multiplexing approach FRET X for FRET via DNA eXchange. We envision that our FRET X technology will be a tool for the high-resolution structural analysis of biomolecules and other nano-structures.

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