Use of Two Technologies, Singulex Single Molecule Counting (SMC)TMand Meso Scale Discovery&[reg](MSD) Technology for Drug Levels Determination of an Antibody Therapeutic, MK-A, in Human Serum in a Dose Escalation Study

2016 ◽  
Author(s):  
Phyllis Conliffe
Keyword(s):  
Human Serum ◽  
Single Molecule ◽  
Dose Escalation ◽  
Meso Scale Discovery ◽  
Dose Escalation Study ◽  
Drug Levels ◽  
Meso Scale

Determination of chemically reduced pyrrolobenzodiazepine SJG-136 in human plasma by HPLC-MS/MS: application to an anticancer phase I dose escalation study

2007 ◽  
Vol 43 (1) ◽  
pp. 42-52 ◽  
Author(s):  
M. Wade Calcutt ◽  
Wooin Lee ◽  
Igor Puzanov ◽  
Mace L. Rothenberg ◽  
David L. Hachey
Keyword(s):  
Phase I ◽  
Human Plasma ◽  
Dose Escalation ◽  
Dose Escalation Study

Determination of drug levels in human serum by circular dichroism

Chirality ◽  
1998 ◽  
Vol 10 (6) ◽  
pp. 507-512 ◽  
Author(s):  
Pilar Gort�zar ◽  
Alfredo Ro�n ◽  
Jes�s T. V�zquez
Keyword(s):  
Circular Dichroism ◽  
Human Serum ◽  
Drug Levels ◽  
Circular Dichroïsm

Micro-Scale Ultracentrifugation as an Alternative to Ultrafiltration for the Determination of the Unbound Fraction of Phenytoin in Human Serum

1986 ◽  
Vol 23 (5) ◽  
pp. 603-607 ◽  
Author(s):  
L Jack ◽  
C Cunningham ◽  
I D Watson ◽  
M J Stewart
Keyword(s):  
Human Serum ◽  
Simple Procedure ◽  
Free Fraction ◽  
Free Drug ◽  
Effect Of Temperature ◽  
Unbound Fraction ◽  
Drug Levels ◽  
Micro Scale ◽  
Great Utility

Free phenytoin has been determined using micro-scale ultracentrifugation followed by analysis by EMIT. The effect of temperature on the determined free fraction was investigated and the ultracentrifugation procedure validated against ultrafiltration. Ultracentrifugation gave free fractions which were on average 16% lower than those obtained using ultrafiltration, but correlation was good, as was the correlation with measurements of total phenytoin ( r=0·90). Micro-scale ultracentrifugation is a simple procedure which can be of great utility in the measurement and investigation of free drug levels.

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