Paula Sofia Coutinho Medeiros
◽
Ana Lúcia Marques Batista de Carvalho
◽
Cristina Ruano
◽
Juan Carlos Otero
◽
Maria Paula Matos Marques
Background:
The impact of the ubiquitous dietary phenolic compound p-coumaric acid
on human breast cancer cells was assessed, through a multidisciplinary approach: Combined biological
assays for cytotoxicity evaluation and biochemical profiling by Raman microspectroscopic
analysis in cells.
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Methods: Para-coumaric acid was shown to exert in vitro chemoprotective and antitumor activities,
depending on the concentration and cell line probed: a significant anti-invasive ability was detected
for the triple-negative MDA-MB-231 cells, while a high pro-oxidant effect was found for the estrogen-
dependent MCF-7 cells. A striking cell selectivity was obtained, with a more noticeable outcome
on the triple-negative MDA-MB-231 cell line.
Results:
The main impact on the cellular biochemical profile was verified to be on proteins and
lipids, thus justifying the compound´s anti-invasive effect and chemoprotective ability.
Conclusion:
p-Coumaric acid was thus shown to be a promising chemoprotective/chemotherapeutic
agent, particularly against the low prognosis triple-negative human breast adenocarcinoma.
6,N2-Diaryl-1,3,5-triazine-2,4-diamines: synthesis, antiproliferative activity and 3D-QSAR modeling