Compartmentalized microfluidic chambers enable long-term maintenance and communication between human pluripotent stem cell-derived forebrain and midbrain neurons

Compartmentalized microfluidic devices are becoming increasingly popular and have proven to be valuable tools to probe neurobiological functions that are inherently difficult to study using traditional approaches. The ability of...

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Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces

Biomaterials ◽

10.1016/j.biomaterials.2010.08.007 ◽

2010 ◽

Vol 31 (34) ◽

pp. 9135-9144 ◽

Cited By ~ 127

Author(s):

David A. Brafman ◽

Chien W. Chang ◽

Antonio Fernandez ◽

Karl Willert ◽

Shyni Varghese ◽

...

Keyword(s):

Stem Cell ◽

Pluripotent Stem Cell ◽

Synthetic Polymer ◽

Polymer Surfaces ◽

Human Pluripotent Stem Cell ◽

Self Renewal

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Efficient long-term expansion of primitive erythroblasts from a human pluripotent stem cell line harbouring the homozygous sickle cell anaemia mutation

Reproductive BioMedicine Online ◽

10.1016/s1472-6483(10)61211-4 ◽

2009 ◽

Vol 18 ◽

pp. S-7

Author(s):

MV Pryzhkova ◽

A Tripathi ◽

A Peters ◽

X Yuan ◽

D Sullivan ◽

...

Keyword(s):

Stem Cell ◽

Cell Line ◽

Sickle Cell ◽

Pluripotent Stem Cell ◽

Sickle Cell Anaemia ◽

Human Pluripotent Stem Cell ◽

Stem Cell Line

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Enhanced Therapeutic and Long-Term Dynamic Vascularization Effects of Human Pluripotent Stem Cell–Derived Endothelial Cells Encapsulated in a Nanomatrix Gel

Circulation ◽

10.1161/circulationaha.116.026329 ◽

2017 ◽

Vol 136 (20) ◽

pp. 1939-1954 ◽

Cited By ~ 30

Author(s):

Shin-Jeong Lee ◽

Young-Doug Sohn ◽

Adinarayana Andukuri ◽

Sangsung Kim ◽

Jaemin Byun ◽

...

Keyword(s):

Endothelial Cells ◽

Stem Cell ◽

Pluripotent Stem Cell ◽

Human Pluripotent Stem Cell

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Evaluation of engraftable human pluripotent stem cell-derived chondrocytes in a long-term preclinical pig model of focal cartilage injury

Osteoarthritis and Cartilage ◽

10.1016/j.joca.2020.02.052 ◽

2020 ◽

Vol 28 ◽

pp. S32

Author(s):

F. Petrigliano ◽

N. Liu ◽

J. Bogdanov ◽

S. Lee ◽

A. Sarkar ◽

...

Keyword(s):

Stem Cell ◽

Pluripotent Stem Cell ◽

Pig Model ◽

Cartilage Injury ◽

Human Pluripotent Stem Cell

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A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)

Acta Biomaterialia ◽

10.1016/j.actbio.2016.10.037 ◽

2017 ◽

Vol 48 ◽

pp. 120-130 ◽

Cited By ~ 9

Author(s):

Divya Varun ◽

Gayathri Rajaram Srinivasan ◽

Yi-Huan Tsai ◽

Hyun-Je Kim ◽

Joshua Cutts ◽

...

Keyword(s):

Stem Cell ◽

Progenitor Cells ◽

Neuronal Differentiation ◽

Pluripotent Stem Cell ◽

Neural Progenitor Cells ◽

Neural Progenitor ◽

Human Pluripotent Stem Cell

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Long-Term Expansion of Functional Human Pluripotent Stem Cell-Derived Hepatic Organoids

International Journal of Stem Cells ◽

10.15283/ijsc20060 ◽

2020 ◽

Vol 13 (2) ◽

pp. 279-286 ◽

Cited By ~ 2

Author(s):

Seon Ju Mun ◽

Yeon-Hwa Hong ◽

Hyo-Suk Ahn ◽

Jae-Sung Ryu ◽

Kyung-Sook Chung ◽

...

Keyword(s):

Stem Cell ◽

Pluripotent Stem Cell ◽

Human Pluripotent Stem Cell

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Long-Term Chronic Toxicity Testing Using Human Pluripotent Stem Cell–Derived Hepatocytes

Drug Metabolism and Disposition ◽

10.1124/dmd.114.059154 ◽

2014 ◽

Vol 42 (9) ◽

pp. 1401-1406 ◽

Cited By ~ 58

Author(s):

Gustav Holmgren ◽

Anna-Karin Sjögren ◽

Isabel Barragan ◽

Alan Sabirsh ◽

Peter Sartipy ◽

...

Keyword(s):

Stem Cell ◽

Pluripotent Stem Cell ◽

Chronic Toxicity ◽

Toxicity Testing ◽

Human Pluripotent Stem Cell

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Pluripotent Stem Cell-derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins

10.1101/460907 ◽

2018 ◽

Cited By ~ 1

Author(s):

Hyosung Kim ◽

Ranjie Xu ◽

Padmashri Ragunathan ◽

Anna Dunaevsky ◽

Ying Liu ◽

...

Keyword(s):

Stem Cell ◽

Human Brain ◽

Pluripotent Stem Cell ◽

Expression Patterns ◽

Neuronal Maturation ◽

Human Pluripotent Stem Cell ◽

Temporal Expression ◽

Functional Differences ◽

Rodent Brain

SUMMARYThe process of oligodendrogenesis has been relatively well delineated in the rodent brain. However, it remains unknown whether analogous developmental processes are manifested in the human brain. Here, we report oligodendrogenesis in forebrain organoids, generated by using OLIG2-GFP knockin human pluripotent stem cell (hPSC) reporter lines. OLIG2/GFP exhibits distinct temporal expression patterns in ventral forebrain organoids (VFOs) vs. dorsal forebrain organoids (DFOs). Interestingly, oligodendrogenesis can be induced in both VFOs and DFOs after neuronal maturation. Assembling VFOs and DFOs to generate fused forebrain organoids (FFOs) promotes oligodendroglia maturation. Furthermore, dorsally-derived oligodendroglial cells outcompete ventrally-derived oligodendroglia and become dominant in FFOs after long-term culture. Thus, our organoid models reveal human oligodendrogenesis with ventral and dorsal origins. These models will serve to study the phenotypic and functional differences between human ventrally- and dorsally-derived oligodendroglia and to reveal mechanisms of diseases associated with cortical myelin defects.

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