Dynamic motions and architectural changes in DNA supramolecular aggregates visualized via transmission electron microscopy without liquid cells

Nanoscale ◽  
2021 ◽  
Vol 13 (37) ◽  
pp. 15928-15936
Author(s):  
Zhuoyang Lu ◽  
Xiangyang Liu ◽  
Maogang He ◽  
Jiangang Long ◽  
Jiankang Liu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ionic Liquids ◽  
Gold Nanoparticle ◽  
Dynamic Processes ◽  
Nanometer Resolution ◽  
Transmission Electron ◽  
Nanoparticle Aggregates ◽  
Supramolecular Aggregates

The nonvolatility and remarkable solvation property of ionic liquids is exploited to image the dynamic processes of DNA supramolecular aggregates and gold nanoparticle aggregates at nanometer resolution in an unsealed manner.

scholarly journals Atomic mechanisms of gold nanoparticle growth in ionic liquids studied by in situ scanning transmission electron microscopy

Nanoscale ◽  
2020 ◽  
Vol 12 (44) ◽  
pp. 22511-22517
Author(s):  
Debora Keller ◽  
Trond R. Henninen ◽  
Rolf Erni
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Ionic Liquids ◽  
Liquid Phase ◽  
Gold Nanoparticle ◽  
Scanning Transmission Electron Microscopy ◽  
Nanoparticle Growth ◽  
Transmission Electron ◽  
Scanning Transmission

This work reveals direct, experimental insights into dynamic, atomic mechanisms of gold nanoparticle growth in liquid phase by electron microscopy.

Imaging Specific Protein Labels on Eukaryotic Cells in Liquid with Scanning Transmission Electron Microscopy

2011 ◽  
Vol 19 (5) ◽  
pp. 16-20 ◽  
Author(s):  
Diana B. Peckys ◽  
Madeline J. Dukes ◽  
Elisabeth A. Ring ◽  
David W. Piston ◽  
Niels de Jonge
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Protein Complexes ◽  
Cellular Function ◽  
Specific Protein ◽  
Intact Cells ◽  
Nanometer Resolution ◽  
Silicon Nitride Membrane ◽  
Transmission Electron ◽  
Novel Concept

Understanding the structure and dynamics of the protein complexes that underlie cellular function is a central scientific challenge. Biochemical techniques used to identify such complexes would be enhanced by the imaging of specific molecular positions in the context of intact cells, with protein-scale resolution (on the order of a few nanometers). Currently, though, nanometer resolution can only be achieved at the cost of less-direct imaging of the unperturbed cell. Cellular ultrastructure is traditionally studied by transmission electron microscopy (TEM), which yields nanometer resolution on embedded and stained sections, or cryo sections. These cellular samples are neither intact nor in their native liquid state. Light microscopy is used to image protein distributions in fluorescently labeled cells in liquid to investigate cellular function, but even recent improvements in resolution by nanoscopy techniques are still insufficient to resolve the individual constituents of protein complexes. Thus, development of techniques capable of high-resolution imaging in native cellular states would contribute significantly to our understanding of cellular function at the molecular level. The development of liquid compartments that include electron-transparent silicon nitride membrane windows has led to the introduction of a novel concept to achieve nanometer resolution on tagged proteins in cells.

A novel bacteriochlorin-gold nanoparticle construct for photoacoustic imaging

2016 ◽  
Vol 20 (01n04) ◽  
pp. 490-496 ◽  
Author(s):  
Avinash Srivatsan ◽  
Mansik Jeon ◽  
Yanfang Wang ◽  
Yihui Chen ◽  
Chulhong Kim ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gold Nanoparticle ◽  
Photoacoustic Imaging ◽  
Water Soluble ◽  
Au Nps ◽  
Transmission Electron ◽  
High Resolution Tem ◽  
Tem Transmission Electron Microscopy

Herein we report the synthesis of new gold nanoparticle-bacteriochlorin conjugate (Au-NP-PS) as a highly efficient photoacoustic (PA) imaging agent. The conjugate was obtained by reacting a water soluble thio-bacteriochlorin (derived from bacteriochlorophyll-[Formula: see text] with Au-NP. The resulting NPs were characterized by UV-vis, TEM (transmission electron microscopy)/HRTEM (high resolution TEM) and diffraction pattern. The results obtained from in vitro studies suggest that due to conjugation between the PS and Au-NPs, the photoacoustic (PA) signals from the Au-NP-PS were greatly enhanced by a factor of 2.4 compared to that from PS alone.

NOVEL METHOD FOR THE SYNTHESIS OF SILOXANE NANOWIRES AND FILAMENTS USING GOLD NANOPARTICLE CATALYSTS

2005 ◽  
Vol 04 (05n06) ◽  
pp. 1007-1010
Author(s):  
B. L. V. PRASAD ◽  
S. STOEVA ◽  
C. M. SORENSEN ◽  
K. J. KLABUNDE ◽  
V. ZAIKOVSKII
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Polymerization Reaction ◽  
Nanoparticle Catalysts ◽  
Transmission Electron ◽  
Elemental Analyses ◽  
Novel Method ◽  
Microscopy Images

A unique polymerization reaction of alkylsilanes to siloxane nanowires, filaments and tubes in presence of gold nanoparticles and water is presented. The gold nanoparticles, which catalyze this reaction also work as templates restricting the shape of the resulting polymers into wires and tubes. High resolution transmission electron microscopy images clearly reveal the presence of gold nanoparticle at the tip of each wire supporting the template hypothesis. Elemental analyses by different methods confirm the stoichiometry to be C 18 H 37 SiO 1.5, when the alkylsilane used was C 18 H 37 SiH 3, thus proving that the alkyl group remains intact during the polymerization reaction.

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