A radiochemical assay for glutamine synthetase, and activity of the enzyme in rat tissues
Keyword(s):
1. A radiochemical assay for glutamine synthetase has been developed in which an ATP-regenerating system is incorporated to prevent accumulation of inhibitory amounts of ADP. It is particularly suitable for assay of the enzyme in crude tissue extracts containing high adenosine triphosphatase activity. 2. A survey of the distribution of the enzyme in tissues from normal male rats showed that activity is present in liver, brain cortex, kidney cortex, spleen, testis and retina. 3. The Km of the enzyme for l-glutamate is approx. 1.5×10−2m.
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1993 ◽
Vol 28
(5)
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pp. 599-619
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Keyword(s):
1980 ◽
Vol 201
(1)
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pp. 147-159
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Keyword(s):
Fibrinolytic Activity of Lung and Spleen Extracts Observed in Conventional but not in Germ-Free Rats
1979 ◽
Vol 42
(02)
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pp. 726-733
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1961 ◽
Vol 7
(1)
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pp. 127-130
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1984 ◽
Vol 259
(7)
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pp. 4230-4236
1984 ◽
Vol 259
(11)
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pp. 7061-7066
1963 ◽
Vol 238
(4)
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pp. 1516-1519
1963 ◽
Vol 238
(9)
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pp. 2945-2949