scholarly journals Stimulation of steroid glucuronidation by uridine diphosphate N-acetylglucosamine and by microsomal disruption

1977 ◽  
Vol 168 (1) ◽  
pp. 125-127 ◽  
Author(s):  
J Wilkinson ◽  
T Hallinan
Keyword(s):  
Uridine Diphosphate ◽  
Glucuronyl Transferase ◽  
Triton X ◽  
Stimulation Of

Glucuronidation of oestrone and of oestradiol in microsomal fractions was markedly and significantly stimulated by UDP-N-acetylglucosamine and by ultrasonication: Triton X-100 also stimulated. This is consistent with compartmentation of UDP-glucuronyl-transferase. Stimulation by UDP-N-acetylglucosamine may be physiologically significant.

Uridine Diphosphate (UDP) Stimulation of Protein Polysaccharide Production

1974 ◽  
Vol 56 (6) ◽  
pp. 1239-1245 ◽  
Author(s):  
MICHAEL G. EHRLICH ◽  
HENRY J. MANKIN ◽  
BENJAMIN V. TREADWELL ◽  
HARDIN JONES
Keyword(s):  
Uridine Diphosphate ◽  
Polysaccharide Production ◽  
Stimulation Of

EGF promotes gastric mucosal restitution by activating Na+/H+exchange of epithelial cells

2002 ◽  
Vol 282 (5) ◽  
pp. G866-G876 ◽  
Author(s):  
Akinori Yanaka ◽  
Hideo Suzuki ◽  
Takeshi Shibahara ◽  
Hirofumi Matsui ◽  
Akira Nakahara ◽  
...  
Keyword(s):  
Electrical Resistance ◽  
Egf Receptor ◽  
Mucous Cells ◽  
Egf Receptors ◽  
Intracellular Acidosis ◽  
Receptor Antibody ◽  
Epidermal Growth ◽  
Triton X ◽  
Stimulation Of

This study was conducted to determine whether the contributions of epidermal growth factor (EGF) to gastric mucosal restitution after injury are mediated by stimulation of Na+/H+exchangers in surface mucous cells (SMC). Intact sheets of guinea pig gastric mucosae were incubated in vitro. Intracellular pH (pHi) in SMC was measured fluorometrically, using 2′,7′- bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein. Restitution after Triton X-100-induced injury was evaluated by recovery of electrical resistance. At neutral luminal pH, exogenous EGF (ex-EGF) increased pHiand enhanced restitution in the absence but not in the presence of serosal HCO[Formula: see text]. During exposure to luminal acid, ex-EGF not only prevented intracellular acidosis but also promoted restitution. These effects of ex-EGF were blocked by serosal amiloride or anti-EGF-receptor antibody. In the absence of ex-EGF, restitution was inhibited by replacement of luminal and serosal solutions with fresh solutions and was blocked more completely by serosal anti-EGF-receptor antibody. These results suggest that both endogenous and ex-EGF contribute to restitution via basolateral EGF receptors, with effects mediated, at least in part, by stimulation of basolateral Na+/H+exchangers.

scholarly journals Respiration of the rumen ciliate Dasytricha ruminantium Schuberg

1982 ◽  
Vol 206 (2) ◽  
pp. 259-266 ◽  
Author(s):  
N Yarlett ◽  
D Lloyd ◽  
A G Williams
Keyword(s):  
Electron Transport ◽  
Endogenous Respiration ◽  
Tritrichomonas Foetus ◽  
Acetyl Phosphate ◽  
O2 Uptake ◽  
Triton X ◽  
Stimulation Of

The endogenous respiration of the rumen ciliate Dasytricha ruminantium maintained under an O2 tension of 2kPa (approximately 0.02 atm) was partially inhibited by KCN (40% inhibition) and NaN3 (58% inhibition). The organisms lack cytochromes, and sensitivity of respiration to KCN, NaN3, chloroquine and quercetin suggest that the operation of flavoprotein-iron-sulphur-mediated electron transport. As in Tritrichomonas foetus, hydrogenosomal respiration can be stimulated by the addition of CoA in the presence of 0.025% Triton X-100; stimulation by ADP was not detected. Stimulation of pyruvate-supported O2 uptake by Pi suggests that acetate is produced via acetyl phosphate.

scholarly journals Stimulation of collagen galactosyltransferase and glucosyltransferase activities by lysophosphatidylcholine

1976 ◽  
Vol 160 (1) ◽  
pp. 29-35 ◽  
Author(s):  
H Anttinen
Keyword(s):  
Chick Embryo ◽  
High Speed ◽  
Membrane Structures ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
Triton X ◽  
Regulatory Significance ◽  
Stimulation Of

Lysophosphatidylcholine stimulated the activities of collagen galactosyl- and glucosyl-transferases in chick-embryo extract and its particulate fractions in vitro, whereas essentially no stimulation was noted in the high-speed supernatant, where the enzymes are soluble and membrane-free. The stimulatory effect of lysophosphatidylcholine was masked by 0.1% Triton X-100. In kinetic experiments lysophosphatidylcholine raised the maximum velocities with respect to the substrates and co-substrates, whereas no changes were observed in the apparant Km values. Phospholipase A preincubation of the chick-embryo extract resulted in stimulation of both transferase activities, probably gy generating lysophosphatides from endogenous phospholipids. No stimulation by lysophosphatidylcholine was found when tested with 500-fold-purified glycosyltransferase. The results suggest that collagen glycosyltransferases must be associated with the membrane structures of the cell in order to be stimulated by lysophosphatidylcholine. Lysophosphatidylcholine could have some regulatory significance in vivo, since its concentration in the cell is comparable with that which produced marked stimulation in vitro.

scholarly journals The γ-aminobutyrate/benzodiazepine receptor from pig brain. Enhancement of γ-aminobutyrate-receptor binding by the anaesthetic propanidid

1986 ◽  
Vol 233 (1) ◽  
pp. 259-264 ◽  
Author(s):  
E F Kirkness ◽  
A J Turner
Keyword(s):  
Gaba Receptor ◽  
Benzodiazepine Receptor ◽  
Functional Characteristic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Gaba Receptor Complex ◽  
Triton X ◽  
Muscimol Binding ◽  
Ic50 Concentration ◽  
Stimulation Of

The binding of [3H]muscimol, a gamma-aminobutyrate (GABA) receptor agonist, to a membrane preparation from pig cerebral cortex was enhanced by the anaesthetic propanidid in a concentration-dependent manner. At 0 degrees C, binding was stimulated to 220% of control values, with 50% stimulation at 60 microM-propanidid. At 37 degrees C, propanidid caused a more powerful stimulation of [3H]muscimol binding (340% of control values). Propanidid (1 mM) exerted little effect on the affinity of muscimol binding (KD approx. 10 nM), but increased the apparent number of high-affinity binding sites in the membrane by 2-fold. Enhancement of [3H]muscimol binding was observed only in the presence of Cl- ions, half-maximal activation being achieved at approx. 40 mM-Cl-. Picrotoxinin inhibited the stimulation of [3H]muscimol binding by propanidid with an IC50 (concentration causing 50% inhibition) value of approx. 25 microM. The enhancement of [3H]muscimol binding by propanidid was not additive with the enhancement produced by secobarbital. Phenobarbital inhibited the effect of propanidid and secobarbital. The GABA receptor was solubilized with Triton X-100 or with Chaps [3-[(3-cholamidopropyl)dimethylammonio]propanesulphonate]. Propanidid and secobarbital did not stimulate the binding of [3H]muscimol after solubilization with Triton X-100. However, the receptor could be solubilized by 5 mM-Chaps with retention of the stimulatory effects of propanidid and secobarbital. Unlike barbiturates, propanidid did not stimulate the binding of [3H]flunitrazepam to membranes. It is suggested that the ability to modulate the [3H]muscimol site of the GABA-receptor complex may be a common and perhaps functional characteristic of general anaesthetics.

scholarly journals Case-controlled study on indirect hyperbilirubinemia in exclusively breast fed neonates and mutations of the bilirubin Uridine Diphosphate-Glucuronyl transferase gene 1A1

2017 ◽  
Vol 13 ◽  
pp. 6-12 ◽  
Author(s):  
Amal E. Mohammed ◽  
Eman G. Behiry ◽  
Akram E. El-Sadek ◽  
Waleed E. Abdulghany ◽  
Dalia M. Mahmoud ◽  
...  
Keyword(s):  
Controlled Study ◽  
Uridine Diphosphate ◽  
Glucuronyl Transferase ◽  
Transferase Gene ◽  
Breast Fed
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