scholarly journals Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys

2004 ◽  
Vol 384 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Shuting LI ◽  
Jingqiang WANG ◽  
Xumin ZHANG ◽  
Yan REN ◽  
Ning WANG ◽  
...  
Keyword(s):  
Southern China ◽  
Gel Filtration ◽  
Complex Mixture ◽  
Tandem Ms ◽  
Post Translational Modification ◽  
Combination Strategy ◽  
Naja Naja ◽  
Maldi Tof ◽  
Proteins And Peptides ◽  
Naja Atra

Snake venom is a complex mixture of proteins and peptides, and a number of studies have described the biological properties of several venomous proteins. Nevertheless, a complete proteomic profile of venom from any of the many species of snake is not available. Proteomics now makes it possible to globally identify proteins from a complex mixture. To assess the venom proteomic profiles from Naja naja atra and Agkistrodon halys, snakes common to southern China, we used a combination strategy, which included the following four different approaches: (i) shotgun digestion plus HPLC with ion-trap tandem MS, (ii) one-dimensional SDS/PAGE plus HPLC with tandem MS, (iii) gel filtration plus HPLC with tandem MS and (iv) gel filtration and 2DE (two-dimensional gel electrophoresis) plus MALDI–TOF (matrix-assisted laser desorption ionization–time-of-flight) MS. In the present paper, we report the novel identification of 124 and 74 proteins and peptides in cobra and viper venom respectively. Functional analysis based upon toxin categories reveals that, as expected, cobra venom has a high abundance of cardio- and neurotoxins, whereas viper venom contains a significant amount of haemotoxins and metalloproteinases. Although approx. 80% of gel spots from 2DE displayed high-quality MALDI-TOF-MS spectra, only 50% of these spots were confirmed to be venom proteins, which is more than likely to be a result of incomplete protein databases. Interestingly, these data suggest that post-translational modification may be a significant characteristic of venomous proteins.

scholarly journals Cardiotoxin II from Taiwan cobra venom, Naja naja atra. Structure in solution and comparison among homologous cardiotoxins.

1994 ◽  
Vol 269 (38) ◽  
pp. 23500-23508
Author(s):  
R. Bhaskaran ◽  
C.C. Huang ◽  
Y.C. Tsai ◽  
G. Jayaraman ◽  
D.K. Chang ◽  
...  
Keyword(s):  
Cobra Venom ◽  
Naja Naja ◽  
Structure In Solution ◽  
Naja Atra ◽  
Cardiotoxin Ii

scholarly journals The Role of the N-Terminal Leucine Residue in Snake Venom Cardiotoxin II (Naja naja atra)

1997 ◽  
Vol 233 (3) ◽  
pp. 713-716 ◽  
Author(s):  
Chi-Yue Wu ◽  
Wan-Chen Chen ◽  
Chewn-Lang Ho ◽  
Shui-Tein Chen ◽  
Kung-Tsung Wang
Keyword(s):  
Snake Venom ◽  
Naja Naja ◽  
Leucine Residue ◽  
Naja Atra ◽  
Cardiotoxin Ii

scholarly journals High index of suspicion for brucellosis in a highly cosmopolitan city in southern China

2019 ◽  
Author(s):  
Hai-Yan Ye ◽  
Fan-Fan Xing ◽  
Jin Yang ◽  
Simon Kam-Fai Lo ◽  
Ricky Wing-Tong Lau ◽  
...  
Keyword(s):  
Risk Factors ◽  
Southern China ◽  
Clinical Specimen ◽  
Guangdong Province ◽  
Human Brucellosis ◽  
Brucella Strain ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Chest Wall Abscess ◽  
Tof Ms

Abstract Background: Brucellosis is one of the most widespread zoonosis in the world. In China, 90% of human brucellosis occurs in six northern agricultural provinces. However, there is a recent increase in the trend of human brucellosis in southern provinces with limited cases reported in the literature. Our study aims to describe the clinical features and epidemiology of brucellosis in a tertiary hospital in southern China. Methods: A retrospective case series of brucellosis was conducted between January 1, 2014 and October 31. 2018. Cases were identified based on positive Brucella serology by tube agglutination test, or positive culture from clinical specimen identified by Vitek 2 and MALDL-TOF MS. Clinical details of brucellosis including patients’ occupation, risk factors, and complications were analyzed. Clinical characteristics between patients from Guangdong and other provinces were also compared. Results: A total of 13 cases of laboratory-confirmed brucellosis were identified. 7 (53.8%) of the patients were male, 6 (46.2%) were female, with age ranging from 29 to 73 years old (median age: 51 years). Five patients (38.5%) were from Guangdong province, while the remaining patients (61.5%) were from other provinces. The commonest risk factors of acquisition were consumption of undercooked meat and goat placenta. Patients from Guangdong province were found to be more likely to have prior placenta consumption and handling of animal meat. The commonest clinical presentations were fever, osteoarticular pain, urinary symptoms, splenomegaly, and lymphadenopathy. Spondylodiscitis/ peripheral joint arthritis (5 patients, 38.5%) was the most prevalent complication, while extra-osteoarticular complications including abdominal aortitis, hepatosplenic abscess, chest wall abscess, and epididymo-orchitis were observed in 4 other patients. Furthermore, it was demonstrated that MALDI-TOF MS is reliable in Brucella identification after additional of reference spectra with standard Brucella strain. Conclusions: Brucellosis, previously thought to be only found in northern China, is now increasingly seen in highly cosmopolitan part of southern China. MALDI-TOF MS in hospitals in China should include reference spectra with standard Brucella strain to aid bacterial identification in routine clinical practice. In addition to tuberculosis, typhoid fever and typhus, brucellosis should be considered in patients with fever of unknown origin in this locality.

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