scholarly journals Cell-type differences in the serum and cyclic AMP-dependent regulation of ornithine decarboxylase activity in two cultured fibroblast types

1982 ◽  
Vol 204 (1) ◽  
pp. 357-360 ◽  
Author(s):  
R J Boucek ◽  
J Weiss ◽  
K J Lembach
Keyword(s):  
Cyclic Amp ◽  
Ornithine Decarboxylase ◽  
Decarboxylase Activity ◽  
Cell Type ◽  
Dibutyryl Cyclic Amp ◽  
Cultured Fibroblasts ◽  
Ornithine Decarboxylase Activity ◽  
Serum Stimulation ◽  
3T3 Fibroblasts ◽  
Stimulation Of

Serum re-feeding stimulated ornithine decarboxylase (ODC) activity 8 to 10-fold in FS fibroblasts and 5 to 8-fold in 3T3 fibroblasts. Addition of dibutyryl cyclic AMP or 3-isobutyl-1-methylxanthine at the time of serum re-feeding further stimulated ODC activity in 3T3 fibroblasts but inhibited the serum stimulation of ODC activity in FS fibroblasts. It is suggested that serum and cyclic AMP independently regulate ODC activity in cultured fibroblasts.

STIMULATION OF ORNITHINE DECARBOXYLASE AND NUCLEAR RNA POLYMERASE ACTIVITY IN RAT LIVER BY GLUCAGON AND DIBUTYRYL CYCLIC AMP

1973 ◽  
Vol 73 (4) ◽  
pp. 794-800 ◽  
Author(s):  
E. Hölttä ◽  
A. Raina
Keyword(s):  
Cyclic Amp ◽  
Rna Polymerase ◽  
Ornithine Decarboxylase ◽  
Polymerase Activity ◽  
Decarboxylase Activity ◽  
Dibutyryl Cyclic Amp ◽  
Ornithine Decarboxylase Activity ◽  
Nuclear Rna ◽  
Rna Polymerase Activity ◽  
Nuclear Rna Polymerase

ABSTRACT The influence of glucagon and dibutyryl cyclic 3′,5′-AMP on ornithine decarboxylase (EC 4.1.1.17) and nuclear RNA polymerase (EC 2.7.7.6) activity of rat liver was studied. Intraperitoneal administration of 0.2 mg of glucagon per 100 g body weight resulted in a four- to six-fold increase in ornithine decarboxylase activity. This enzyme activity was also markedly increased by a single ip injection containing 2.5–5 mg of dibutyryl cyclic AMP. The time course of this effect demonstrated a maximum at 3–4 h. Both agents also stimulated nuclear RNA polymerase activity. The increase in hepatic ornithine decarboxylase activity by dibutyryl cyclic AMP was not abolished by adrenalectomy but was totally prevented by the administration of actinomycin D, α-amanitin and cycloheximide, which suggests that the increase in activity represents synthesis de novo of the enzyme. A sharp rise in liver ornithine decarboxylase activity was also observed after administration of theophylline, whereas no change in enzyme activity was found after the administration of adenosine or adenosine 5′-nucleotides.

Puromycin Stimulation of Rat Liver Ornithine Decarboxylase Activity

Nature ◽  
1973 ◽  
Vol 241 (5387) ◽  
pp. 275-277 ◽  
Author(s):  
WILLIAM T. BECK ◽  
RILL ANN BELLANTONE ◽  
E. S. CANELLAKIS
Keyword(s):  
Ornithine Decarboxylase ◽  
Rat Liver ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity ◽  
Stimulation Of
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