scholarly journals Intramolecular distances between tryptophan residues and the active-site serine residue in alkaline bacterial proteinases as measured by fluorescence energy-transfer studies

1983 ◽  
Vol 215 (2) ◽  
pp. 413-416 ◽  
Author(s):  
N C Genov ◽  
M Shopova ◽  
R Boteva ◽  
F Ricchelli ◽  
G Jori
Keyword(s):  
Energy Transfer ◽  
Active Site ◽  
Three Dimensional ◽  
The Other ◽  
Fluorescence Energy Transfer ◽  
Serine Residue ◽  
Tryptophan Residues ◽  
The One ◽  
Fluorescence Energy

Singlet-singlet energy transfer from the tryptophan residues to an active-site-serine-bound 5-dimethylaminonaphthalene-1-sulphonyl group was investigated in four subtilisins. The transfer distances for subtilisin Novo and mesentericopeptidase are 1.93 +/- 0.20 nm (19.3 +/- 2.0 A) and 1.81 +/- 0.20 nm (18.1 +/- 2.0 A) respectively. The positions of the indole groups in the three-dimensional structures of the two pairs of proteinases, namely subtilisin Novo and mesentericopeptidase on the one hand and subtilisins Carlsberg and DY on the other, are essentially identical.

scholarly journals Fluorescence energy transfer studies on the active site of papain

1980 ◽  
Vol 77 (2) ◽  
pp. 940-943 ◽  
Author(s):  
J. B. Henes ◽  
M. S. Briggs ◽  
S. G. Sligar ◽  
J. S. Fruton
Keyword(s):  
Energy Transfer ◽  
Active Site ◽  
Fluorescence Energy Transfer ◽  
Fluorescence Energy

scholarly journals Heritage Artefacts in the COVID-19 Era: The Aura and Authenticity of 3D Models

2021 ◽  
Vol 7 (1) ◽  
pp. 519-539
Author(s):  
Thiago Minete Cardozo ◽  
Costas Papadopoulos
Keyword(s):  
User Experience ◽  
Three Dimensional ◽  
3D Models ◽  
Essential Elements ◽  
The Other ◽  
Social Distancing ◽  
The Public ◽  
Affective Experiences ◽  
The One ◽  
Digital Engagement

Abstract Museums have been increasingly investing in their digital presence. This became more pressing during the COVID-19 pandemic since heritage institutions had, on the one hand, to temporarily close their doors to visitors while, on the other, find ways to communicate their collections to the public. Virtual tours, revamped websites, and 3D models of cultural artefacts were only a few of the means that museums devised to create alternative ways of digital engagement and counteract the physical and social distancing measures. Although 3D models and collections provide novel ways to interact, visualise, and comprehend the materiality and sensoriality of physical objects, their mediation in digital forms misses essential elements that contribute to (virtual) visitor/user experience. This article explores three-dimensional digitisations of museum artefacts, particularly problematising their aura and authenticity in comparison to their physical counterparts. Building on several studies that have problematised these two concepts, this article establishes an exploratory framework aimed at evaluating the experience of aura and authenticity in 3D digitisations. This exploration allowed us to conclude that even though some aspects of aura and authenticity are intrinsically related to the physicality and materiality of the original, 3D models can still manifest aura and authenticity, as long as a series of parameters, including multimodal contextualisation, interactivity, and affective experiences are facilitated.

Red Laser-Induced Fluorescence Energy Transfer in an Immunosystem

2000 ◽  
Vol 280 (2) ◽  
pp. 272-277 ◽  
Author(s):  
Bernhard Oswald ◽  
Frank Lehmann ◽  
Lydia Simon ◽  
Ewald Terpetschnig ◽  
Otto S. Wolfbeis
Keyword(s):  
Energy Transfer ◽  
Laser Induced Fluorescence ◽  
Fluorescence Energy Transfer ◽  
Fluorescence Energy

scholarly journals Fluorescence energy transfer in quantum dot/azo dye complexes in polymer track membranes

2013 ◽  
Vol 8 (1) ◽  
pp. 452 ◽  
Author(s):  
Yulia A Gromova ◽  
Anna O Orlova ◽  
Vladimir G Maslov ◽  
Anatoly V Fedorov ◽  
Alexander V Baranov
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Azo Dye ◽  
Fluorescence Energy Transfer ◽  
Track Membranes ◽  
Fluorescence Energy
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