scholarly journals An analysis of the stopped-flow kinetics of gaseous ligand uptake and release by adult mouse erythrocytes

1989 ◽  
Vol 260 (1) ◽  
pp. 171-176 ◽  
Author(s):  
T Brittain ◽  
R Simpson

Ligand uptake and release by the haemoglobin contained within adult mouse erythrocytes was studied by using dual-wavelength stopped-flow techniques. The rate of O2 uptake is very much lower than that expected for an equivalent concentration of haemoglobin in free solution. The O2-concentration-dependence found in uptake experiments is greater than first-order. CO uptake shows the same pattern of reactivity as does O2, but the associated rates of uptake are lower and the concentration-dependence of the CO rates is first-order. O2 release from the adult erythrocytes was measured by stopped-flow mixing with Na2S2O4. Under these circumstances the deoxygenation of intracellular haemoglobin shows accelerating time courses. The apparent rate-constant-dependence on dithionite concentration shows a rate limit at high reductant concentrations. Computer simulations of both ligand uptake and release processes were carried out by using a three-dimensional model. The simulations clearly indicate that in rapid-mixing experiments the rather slow experimentally observed O2 uptake rate is due to rate-limiting diffusion through an extracellular stagnant solvent layer. In the case of O2 release, however, the major rate-controlling process is the rate of O2 dissociation from the haemoglobin molecules, which accelerates during the deoxygenation process.

1989 ◽  
Vol 261 (3) ◽  
pp. 1009-1013 ◽  
Author(s):  
T Brittain ◽  
R Simpson

The processes of O2 uptake and release by the three embryonic haemoglobins contained within early mouse embryonic red blood cells have been studied using dual-wavelength stopped-flow kinetic spectroscopy. The rate of O2 uptake in the pseudo-spherical, nucleated, embryonic red blood cells exhibits a greater than first-order dependence on O2 concentration. The time courses for the release from the red blood cells into dithionite-containing solutions tends towards a limiting rate at high dithionite concentrations. The rates of both the uptake and release processes observed in the embryonic cells are compared with those previously seen for adult mouse red blood cells. A new mathematical model is described which accurately simulates both uptake and release experimental data for the nucleated embryonic red blood cells.


1985 ◽  
Vol 58 (4) ◽  
pp. 1215-1224 ◽  
Author(s):  
K. Yamaguchi ◽  
D. Nguyen-Phu ◽  
P. Scheid ◽  
J. Piiper

The kinetics of O2 uptake into and release from human erythrocytes was investigated at 37 degrees C by a stopped-flow technique. From the time course of O2 saturation (SO2) change a specific transfer conductance of erythrocytes for O2 (GO2) was calculated. The following results were obtained: 1) GO2 decreased in the course of O2 uptake, but initial GO2 was nearly independent of SO2 at which uptake started; 2) addition of albumin to the medium reduced GO2; 3) increasing dithionite concentration in the medium in O2-release experiments progressively enhanced GO2, which became virtually constant for nearly the entire course of release; and 4) O2 uptake and O2 release (without dithoite) in the same SO2 range yielded very similar GO2. These results suggested that O2 uptake and release were importantly limited by diffusion through the external medium and that in the SO2 range between 0.3 and 0.8, chemical reaction exerted little limiting effect. Since O2 release at the highest dithionite concentration (40 mmol/l) appeared to be virtually unlimited by external diffusion, GO2 measured under these conditions, averaging 8.7 ml X min-1 X Torr-1 X ml erythrocytes-1, was considered to mainly reflect intracellular diffusion limitation. The corresponding specific transfer conductance for O2 transfer in whole blood (hematocrit, 0.45) is 3.9 ml X min-1 X Torr-1 X ml blood-1.


Flow maldistributions and corresponding hot spots may cause adverse effects in the operation of down-flow packed-bed reactors. To avoid these phenomena it is necessary to know when and how they occur. In this work, a three-dimensional model that considers the effects of fluid flow, mass transfer by diffusion, heat transfer by conduction and reactant consumption by an exothermic irreversible first-order reaction is used to analyse an adiabatic packed-bed reactor with down-flow. It is shown that the one-dimensional uniform down-flow can become unstable to three-dimensional stationary and time-dependent perturbations, giving rise to non-uniform flow fields that lead to fixed as well as moving hot spots. The boundary of the region of the operating conditions at which these instabilities occur is determined as a function of the various physicochemical parameters that characterize the packed-bed reactor.


1978 ◽  
Vol 18 (05) ◽  
pp. 369-383 ◽  
Author(s):  
K.H. Coats

Abstract This paper describes a three-dimensional, highly implicit numerical model for simulating steamflooding with distillation or solution gas. The model uses direct solution to solve simultaneously three and four equations for the dead oil and two-component oil cases, respectively. The model is compared in stability and computing time with a model reported earlier. The paper includes comparative discussion of alternate steamflood model formulations, one of which we have adopted as a highly stable, isothermal, black-oil model formulation. Introduction A brief review of published descriptions of steamflood models is given in an earlier paper. That paper described a partially compositional, three-dimensional model that solves first a single-variable pressure equation, then two simultaneous saturation equations. In our experience with dead-oil steamflood problems, that model exhibits adequate stability in most cases and marginal stability in some cases. In some compositional problems, the formulation of that model leads to problems, the formulation of that model leads to deteriorating material balances for light hydrocarbon components. The model described here was developed to gain improved stability for all types of steamflood problems and to eliminate the material balance problems and to eliminate the material balance shortcoming of the earlier model formulation in compositional problems. This highly implicit, three-dimensional model treats oil as a two-component mixture to accommodate problems involving solution or inert gas or distillation. The model simultaneously solves three equations for the dead-oil case and four equations for the compositional case. Transmissibilities, capillary pressures, and production terms are treated pressures, and production terms are treated implicitly in saturations and composition; they also are treated implicitly in temperature in grid blocks where no free gas is present. The term "implicit" refers to evaluation of interblock flow terms and production rates at the new time level, n + 1. We have found insensitivity to explicit or implicit dating of molar densities and viscosities in these terms and therefore simply evaluate them explicitly. We evaluate relative permeabilities at time level n + 1 by the first-order permeabilities at time level n + 1 by the first-order approximation, which ignores second- and higher-order Taylor series terms . Temperature dependence of relative permeability, if present, is treated explicitly. We present the model equations, and describe the highly implicit formulation and method for solution. This model is compared with the earlier steamflood model in stability and efficiency through discussion and example field problems. MODEL DESCRIPTION BASIC EQUATIONS The model consists of five equations expressing conservation of energy, conservation of mass, and phase equilibrium. The mass conservation equations phase equilibrium. The mass conservation equations apply to water and to two hydrocarbon components. In finite-difference form, these equations are Energy Balance (1) Mass Balance on H2O (2) SPEJ P. 369


2011 ◽  
Vol 7 (S283) ◽  
pp. 184-187
Author(s):  
Sze-Ning Chong ◽  
Sun Kwok ◽  
Hiroshi Imai ◽  
Daniel Tafoya

AbstractWe present a general three-dimensional model of multipolar planetary nebulae (PNe). By rotating to different viewing angles and adjusting the angles between the multiple lobes, we demonstrate that the model is able to reproduce HST Hα images of 20 multipolar young PNe. Though this model only considers the geometrical projection effects, it significantly unifies the selected PNe and can be considered as a first-order fundamental model of the “multipolar” morphological class. This kind of model reduces complexity and is essential to pursuing of the shaping mechanism. In addition, we illustrate that under some special conditions, i.e. in certain viewing angles, or with low sensitivity, it will be hard to imagine that the projected image originates from a multipolar-lobed model.


Skull Base ◽  
2008 ◽  
Vol 18 (S 01) ◽  
Author(s):  
Akio Morita ◽  
Toshikazu Kimura ◽  
Shigeo Sora ◽  
Kengo Nishimura ◽  
Hisayuki Sugiyama ◽  
...  

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