scholarly journals Structures of diphospho-myo-inositol pentakisphosphate and bisdiphospho-myo-inositol tetrakisphosphate from Dictyostelium resolved by NMR analysis

1996 ◽  
Vol 315 (3) ◽  
pp. 715-720 ◽  
Author(s):  
Tim LAUSSMANN ◽  
Reint EUJEN ◽  
C. Michael WEISSHUHN ◽  
Ulrich THIEL ◽  
Günter VOGEL
Keyword(s):  
31P Nmr ◽  
Inositol Phosphates ◽  
Phosphate Group ◽  
Two Dimensional ◽  
Nmr Analysis ◽  
Group Transfer ◽  
Inositol Tetrakisphosphate ◽  
Positional Isomerism ◽  
Transfer Potential ◽  
High Phosphate

Diphospho-myo-inositol phosphates (PP-InsP5 and bis-PP-InsP4) were isolated from Dictyostelium in order to clarify the precise positional isomerism by two-dimensional 1H/31P-NMR analysis. The diphosphorylated inositol phosphates are 4-PP-Ins(1,2,3,5,6)P5 and 4,5-bis-PP-Ins(1,2,3,6)P4 or their corresponding enantiomers. The vicinal arrangement of the diphospho groups with its steric and electrostatic constraints possibly qualifies bis-PP-InsP4 as a metabolite with high phosphate-group-transfer potential in phosphotransferase reactions.

scholarly journals Diphospho-myo-inositol phosphates from Dictyostelium identified as d-6-diphospho-myo-inositol pentakisphosphate and d-5,6-bisdiphospho-myo-inositol tetrakisphosphate

1997 ◽  
Vol 322 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Tim LAUSSMANN ◽  
Komandla Malla REDDY ◽  
K. Kishta REDDY ◽  
J. R. FALCK ◽  
Günter VOGEL
Keyword(s):  
31P Nmr ◽  
Inositol Phosphates ◽  
Two Dimensional ◽  
Nmr Analysis ◽  
Naturally Occurring ◽  
Inositol Tetrakisphosphate

Two diphospho-myo-inositol phosphates from Dictyostelium were recently investigated by two-dimensional 1H/31P NMR analysis and assigned to be either d-4-diphospho-myo-inositol pentakisphosphate (d-4-PP-InsP5) and d-4,5-bisdiphospho-myo-inositol tetrakisphosphate (d-4,5-bis-PP-InsP4) or their corresponding enantiomers d-6-PP-InsP5 and d-5,6-bis-PP-InsP4. In the present study the naturally occurring enantiomers were identified by using defined synthetic PP-InsP5 isomers as substrates for a partially purified PP-InsP5 5-kinase from Dictyostelium. This enzyme specifically phosphorylates the naturally occurring PP-InsP5 and the synthetic d-6-PP-InsP5, leading to d-5,6-bis-PP-InsP4. In contrast, neither d-4-PP-InsP5 nor d-1-PP-InsP5 or d-3-PP-InsP5 are converted by the enzyme.

scholarly journals 31P-NMR analysis of Entamoeba histolytica. Occurrence of high amounts of two inositol phosphates

1993 ◽  
Vol 214 (3) ◽  
pp. 711-718 ◽  
Author(s):  
Jean-Baptiste MARTIN ◽  
Tilly BAKKER-GRUNWALD ◽  
Gerard KLEIN
Keyword(s):  
Entamoeba Histolytica ◽  
31P Nmr ◽  
Inositol Phosphates ◽  
Nmr Analysis

TLC and 31P-NMR Analysis of Low Polarity Phospholipids

Lipids ◽  
2008 ◽  
Vol 44 (4) ◽  
pp. 381-389 ◽  
Author(s):  
Mikhail Vyssotski ◽  
Andrew MacKenzie ◽  
Dawn Scott
Keyword(s):  
31P Nmr ◽  
Nmr Analysis

Mineralisation of soil orthophosphate monoesters under pine seedlings and ryegrass

Soil Research ◽  
2004 ◽  
Vol 42 (2) ◽  
pp. 189 ◽  
Author(s):  
C. R. Chen ◽  
L. M. Condron ◽  
B. L. Turner ◽  
N. Mahieu ◽  
M. R. Davis ◽  
...  
Keyword(s):  
31P Nmr ◽  
Inositol Phosphates ◽  
Radiata Pine ◽  
31P Nmr Spectroscopy ◽  
Organic P ◽  
Soil P ◽  
Predominant Species ◽  
Total Soil ◽  
Pine Seedlings ◽  
Solution 31P Nmr Spectroscopy

The effects of radiata pine (Pinus radiata D. Don) seedlings and ryegrass (Lolium perenne L.) on the mineralisation of orthophosphate monoesters in 7 grassland soils were assessed in a 10-month pot trial using NaOH–EDTA extraction and solution 31P NMR spectroscopy. Extraction with NaOH–EDTA recovered 46–86% of the total soil P, and NaOH–EDTA-extractable organic P determined by molybdate colourimetry ranged between 194 and 715 mg/kg soil, representing 34–85% of the total soil organic P. Orthophosphate monoesters were the predominant species of the extracted organic P in all soils, with much smaller concentrations of orthophosphate diesters, and traces of phosphonates. Concentrations of orthophosphate monoesters were consistently lower in soils under pine (103–480 mg P/kg soil) compared with the initial soils (142–598 mg P/kg soil) and most soils under grass (122–679 mg/kg soil). Mineralisation of myo-inositol hexakisphosphate accounted for 18–100% of the total mineralisation of orthophosphate monoesters in most soils under radiata pine. This suggests that supposedly recalcitrant inositol phosphates are available for uptake by radiata pine, although the extent of this varies among soils.

Overestimation of orthophosphate monoesters in lake sediment by solution 31P-NMR analysis

2017 ◽  
Vol 24 (32) ◽  
pp. 25469-25474 ◽  
Author(s):  
Wenqiang Zhang ◽  
Xin Jin ◽  
Wenzhong Tang ◽  
Baoqing Shan
Keyword(s):  
Lake Sediment ◽  
31P Nmr ◽  
Nmr Analysis
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