A Direct Enzymic Assay for 7α-Hydroxy Bile Acids and Their Conjugates

1973 ◽  
Vol 44 (1) ◽  
pp. 95-98 ◽  
Author(s):  
G. A. D. Haslewood ◽  
G. M. Murphy ◽  
Judith M. Richardson
Keyword(s):  
Thin Layer ◽  
Bile Acids ◽  
Thin Layer Chromatography ◽  
Biological Fluids ◽  
Hydroxysteroid Dehydrogenase ◽  
Layer Chromatography ◽  
Enzymic Assay ◽  
Bile Acids And Salts ◽  
Secondary Bile Acids

1. A 7α-hydroxysteroid dehydrogenase preparation isolated from a strain of Escherichia coli provides a specific and rapid means for the determination of 7α-hydroxy bile acids and their conjugates. 2. When used in conjunction with 3α-hydroxysteroid dehydrogenase the method enables the concentrations of primary and secondary bile acids and salts in biological fluids to be determined directly without the use of thin-layer chromatography. 3. When used with thin-layer chromatography the method allows the specific quantitative determination of chenodeoxycholic acid or its conjugates in the presence of deoxycholic acid and its derivatives.

scholarly journals Determination of individual bile acids in biological fluids by thin-layer chromatography and fluorimetry

1970 ◽  
Vol 23 (4) ◽  
pp. 309-314 ◽  
Author(s):  
D. Panveliwalla ◽  
B. Lewis ◽  
I. D. P. Wootton ◽  
S. Tabaqchali
Keyword(s):  
Thin Layer ◽  
Bile Acids ◽  
Thin Layer Chromatography ◽  
Biological Fluids ◽  
Layer Chromatography

scholarly journals A Simplified Procedure for the Isolation of Bile Acids from Serum Based on a Batch Extraction with the Non-Ionic Resin—Amberlite XAD-7

1977 ◽  
Vol 14 (1-6) ◽  
pp. 235-239 ◽  
Author(s):  
S. Barnes ◽  
A. Chitranukroh
Keyword(s):  
Liquid Chromatography ◽  
Bile Acid ◽  
Thin Layer ◽  
Bile Acids ◽  
Thin Layer Chromatography ◽  
Hydroxysteroid Dehydrogenase ◽  
Gas Liquid Chromatography ◽  
Reproducible Method ◽  
Simplified Procedure ◽  
Layer Chromatography

A simple and reproducible method using the non-ionic resin, Amberlite XAD-7, for the isolation of bile acids from serum by a batch procedure is described. Recoveries were greater than 95% for the non-sulphated bile acids and greater than 70% for the sulphate esters of bile acids. By using 1 g of resin, recoveries were independent of the mass (0.1–5 μmol) of the bile acid present. Up to 35 samples a day can be extracted without requiring dedication of the operator. When serum extracts were analysed by the 3α-hydroxysteroid dehydrogenase procedure for estimation of bile acids, virtually all the background fluorescence was eliminated. These extracts were also suitable for gas liquid chromatography, thin layer chromatography, and radioimmunoassay.

Sign in / Sign up

Export Citation Format

Share Document