Quantitative Determination of Bile Acids and Their Conjugates Using Thin-layer Chromatography and a Purified 3α-Hydroxysteroid Dehydrogenase

1974 ◽  
Vol 9 (3) ◽  
pp. 249-254 ◽  
Author(s):  
O. Fausa ◽  
B. A. Skålhegg
Keyword(s):  
Thin Layer ◽  
Quantitative Determination ◽  
Bile Acids ◽  
Thin Layer Chromatography ◽  
Hydroxysteroid Dehydrogenase ◽  
Layer Chromatography

A Direct Enzymic Assay for 7α-Hydroxy Bile Acids and Their Conjugates

1973 ◽  
Vol 44 (1) ◽  
pp. 95-98 ◽  
Author(s):  
G. A. D. Haslewood ◽  
G. M. Murphy ◽  
Judith M. Richardson
Keyword(s):  
Thin Layer ◽  
Bile Acids ◽  
Thin Layer Chromatography ◽  
Biological Fluids ◽  
Hydroxysteroid Dehydrogenase ◽  
Layer Chromatography ◽  
Enzymic Assay ◽  
Bile Acids And Salts ◽  
Secondary Bile Acids

1. A 7α-hydroxysteroid dehydrogenase preparation isolated from a strain of Escherichia coli provides a specific and rapid means for the determination of 7α-hydroxy bile acids and their conjugates. 2. When used in conjunction with 3α-hydroxysteroid dehydrogenase the method enables the concentrations of primary and secondary bile acids and salts in biological fluids to be determined directly without the use of thin-layer chromatography. 3. When used with thin-layer chromatography the method allows the specific quantitative determination of chenodeoxycholic acid or its conjugates in the presence of deoxycholic acid and its derivatives.

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