Acetylcholine Stimulates Secretion of Chloride and Bicarbonate through Cystic Fibrosis Transmembrane Conductance Regulator in An Airway Epithelial Cell Line

2003 ◽  
Vol 104 (s49) ◽  
pp. 59P-60P
Author(s):  
Lisa Finlay ◽  
S. K. Inglis ◽  
S.M. Wilson ◽  
R.E. Olver
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cell ◽  
Cell Line ◽  
Airway Epithelial Cell ◽  
Epithelial Cell Line ◽  
Transmembrane Conductance Regulator ◽  
Airway Epithelial ◽  
Transmembrane Conductance ◽  
Cystic Fibrosis Transmembrane

scholarly journals Stimulation of chloride secretion by P1 purinoceptor agonists in cystic fibrosis phenotype airway epithelial cell line CFPEo-

1994 ◽  
Vol 112 (1) ◽  
pp. 169-175 ◽  
Author(s):  
Anthony C. Chao ◽  
Jonathan B. Zifferblatt ◽  
John A. Wagner ◽  
Y.-J. Dong ◽  
Dieter C. Gruenert ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cell ◽  
Cell Line ◽  
Airway Epithelial Cell ◽  
Chloride Secretion ◽  
Epithelial Cell Line ◽  
Airway Epithelial ◽  
Stimulation Of

Proinflammatory cytokine responses toP. aeruginosainfection in human airway epithelial cell lines

2001 ◽  
Vol 280 (3) ◽  
pp. L493-L502 ◽  
Author(s):  
Dianne Kube ◽  
Ute Sontich ◽  
David Fletcher ◽  
Pamela B. Davis
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Proinflammatory Cytokine ◽  
Airway Epithelial Cell ◽  
Cytokine Response ◽  
Transmembrane Conductance Regulator ◽  
Airway Epithelial ◽  
Transmembrane Conductance ◽  
Epithelial Cell Lines

A tendency toward excessive inflammation in cystic fibrosis (CF) patients often accompanies lung infections with Pseudomonas aeruginosa. We tested the cytokine response to P. aeruginosa in two pairs of human airway epithelial cell lines matched except for CF transmembrane conductance regulator activity. The 9/HTEo−CF-phenotypic cell line produced significantly more interleukin (IL)-8, IL-6, and granulocyte-macrophage colony-stimulating factor but not regulated on activation normal T cell expressed and secreted (RANTES) in response to Pseudomonas than the 9/HTEo−control line, and the differences widened over time. Similarly, a 16HBE cell line lacking transmembrane conductance regulator activity showed enhanced IL-8 and IL-6 responses compared with the control cell line. The pharmacology of the cytokine response also differed because dexamethasone reduced cytokine production to similar levels in the matched cell lines. The protracted proinflammatory cytokine response of the CF-phenotypic cell lines suggests that the limiting mechanisms of normal cells are absent or attenuated. These results are consistent with in vivo observations in patients with CF and suggest that our novel cell lines may be useful for further investigation of the proinflammatory responses in CF airways.

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