Effect of prothrombin and its activation fragments on calcium oxalate crystal growth and aggregation in undiluted human urine in vitro: relationship between protein structure and inhibitory activity

2002 ◽  
Vol 102 (4) ◽  
pp. 425 ◽  
Author(s):  
Phulwinder K. GROVER ◽  
Rosemary L. RYALL
Keyword(s):  
Crystal Growth ◽  
Protein Structure ◽  
Calcium Oxalate ◽  
Human Urine ◽  
Inhibitory Activity ◽  
Calcium Oxalate Crystal

scholarly journals FKBP-12 Exhibits an Inhibitory Activity on Calcium Oxalate Crystal Growth in Vitro

2002 ◽  
Vol 17 (1) ◽  
pp. 41 ◽  
Author(s):  
In Sook Han ◽  
Yasushi Nakagawa ◽  
Jong Wook Park ◽  
Min Ho Suh ◽  
Sung IL Suh ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Inhibitory Activity ◽  
Calcium Oxalate Crystal

Mannan-binding lectin (MBL)-associated plasma protein present in human urine inhibits calcium oxalate crystal growth

FEBS Letters ◽  
1999 ◽  
Vol 462 (1-2) ◽  
pp. 89-93 ◽  
Author(s):  
Insug Kang ◽  
Jin-Il Kim ◽  
Sung-Goo Chang ◽  
Sun-Ju Lee ◽  
Sang-Lim Choi ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Plasma Protein ◽  
Human Urine ◽  
Calcium Oxalate Crystal ◽  
Mannan Binding Lectin ◽  
Binding Lectin

An unidentified macromolecular inhibitory constituent of calcium oxalate crystal growth in human urine

1990 ◽  
Vol 18 (6) ◽  
pp. 373-379 ◽  
Author(s):  
S. S�rensen ◽  
K. Hansen ◽  
S. Bak ◽  
S. J. Justesen
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Human Urine ◽  
Calcium Oxalate Crystal

scholarly journals ISOLATION OF URINARY HEPARAN SULFATE AND CHONDROITIN SULFATE AND THEIR INHIBITORY ACTIVITY OF CALCIUM OXALATE CRYSTAL GROWTH

1991 ◽  
Vol 82 (3) ◽  
pp. 405-411 ◽  
Author(s):  
Hidenobu Iwata ◽  
Takashi Terado ◽  
Masahiro Kin ◽  
Shunji Nishio ◽  
Masafumi Takeuchi ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Heparan Sulfate ◽  
Chondroitin Sulfate ◽  
Inhibitory Activity ◽  
Calcium Oxalate Crystal

Acidic peptide and polyribonucleotide crystal growth inhibitors in human urine

1977 ◽  
Vol 233 (5) ◽  
pp. F455-F463 ◽  
Author(s):  
H. Ito ◽  
F. L. Coe
Keyword(s):  
Crystal Growth ◽  
Calcium Oxalate ◽  
Human Urine ◽  
Gel Filtration ◽  
Subsequent Treatment ◽  
Calcium Oxalate Crystal ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Acidic Peptide ◽  
Gastric Pepsin

Urine contains nondialyzable inhibitors of calcium oxalate crystal growth. We have pursued the hypothesis that these inhibitors may, in part, be acidic peptides and polyribonucleotide fragments. Homopolyribonucleotides and RNA inhibit calcium oxalate crystal growth at 5 x 10(-6) M of constituent ribonucleotide, whereas the monomer nucleotides are inactive at 10(-4) M. Poly-L-aspartic or glutamic acid are also inhibitory at 5 X 10(-6) M of amino acid, whereas the monomeric amino acids are inert. Gastric pepsin, a naturally occurring acidic peptide, is inhibitory. Incubation with nonspecific protease reduced the inhibitory effectiveness of normal human urine consistently and significantly, a fact compatible with an important contribution of peptides. A variable additional reduction was produced by subsequent treatment with ribonuclease, suggesting only a small role for polyribonucleotide. Sequential ion exchange and gel filtration chromatography and preparative disc gel electrophoresis yielded inhibitory material enriched with peptides that were strongly acidic and high in proline. Peptides and ribonucleotides seem to contribute to urinary nondialyzable crystal growth inhibitory activity.

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