Eosinophil protein X and eosinophil cationic protein as indicators of intestinal inflammation in infants with atopic eczema and food allergy

To investigate whether eosinophils are stimulatedin vivoor have acquired an increased susceptibility to stimuli from the coagulation cascade, the release of eosinophil proteins was compared for three groups of donors with different levels of serum IgE. (1) with atopic dermatitis (s-IgE > 5000 IU/ml,n= 11); (2) with inhalant allergy (200 < s-IgE < 2 000 IU/ml,n= 10); and (3) non-allergic (s- IgE < 100 IU/ml,n= 10). The levels of eosinophil cationic protein and eosinophil protein X (ECP, EPX) were determined in serum (clotting time = 2.0 h) and plasma. Serum and plasma ECP in normal donors demonstrated large intra-personal variations (C.V. 50–80%), but serum-ECP (mean 8.1 ng/ml) was clearly distinguishable from plasma ECP (mean 1.0 ng/ml) by a factor of 8 (range: 5.6–11.6). The ECP released during clotting was markedly increased in the atopic dermatitis group (serum:plasma ratio 13.5,p<0.003) compared with the other groups (6.7 and 5.6). EPX, having a higher plasma level, demonstrated a less pronounced release (serum: plasma ratios 2.0, 1.7 and 1.4), with no statistical difference between donor groups. Considering all donors together the levels of ECP and EPX in plasma and in serum were correlated to the number of eosinophils (coefficients of correlation 0.54-0.58,p<0.002).

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Eosinophil cationic protein(ECP) and eosinophil protein X (EXP) concentrations in serum and bronchial lavage fluid in asthma. effect of prednisolone treatment.

Clinical & Experimental Allergy ◽

10.1111/j.1365-2222.1995.tb03259.x ◽

1995 ◽

Vol 25 (11) ◽

pp. 1118-1127 ◽

Cited By ~ 41

Author(s):

D. S. Robinson ◽

B. Assoufi ◽

S. R. Durham ◽

A. B. Kay

Keyword(s):

Eosinophil Cationic Protein ◽

Lavage Fluid ◽

Cationic Protein ◽

Bronchial Lavage ◽

Prednisolone Treatment ◽

Protein X

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Eosinophil cationic protein and eosinophil protein X: Human amniotic fluid concentrations and gestational tissue content at term

Placenta ◽

10.1016/s0143-4004(98)90007-8 ◽

1998 ◽

Vol 19 (2-3) ◽

pp. 181-185 ◽

Cited By ~ 1

Author(s):

G.E. Rice ◽

C.M. Reimert ◽

K. Bendtzen

Keyword(s):

Amniotic Fluid ◽

Eosinophil Cationic Protein ◽

Human Amniotic Fluid ◽

Cationic Protein ◽

Tissue Content ◽

Protein X

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Fecal Eosinophil Cationic Protein Is a Diagnostic and Predictive Biomarker in Young Adults with Inflammatory Bowel Disease

Journal of Clinical Medicine ◽

10.3390/jcm8122025 ◽

2019 ◽

Vol 8 (12) ◽

pp. 2025 ◽

Cited By ~ 3

Author(s):

Nada Abedin ◽

Teresa Seemann ◽

Sandra Kleinfeld ◽

Jessica Ruehrup ◽

Stefani Röseler ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Food Allergy ◽

Bowel Disease ◽

Eosinophil Cationic Protein ◽

Young Patients ◽

Area Under The Curve ◽

Predictive Biomarker ◽

Fecal Calprotectin ◽

Cationic Protein ◽

Inflammatory Bowel

Background and Aims: Fecal biomarkers are important non-invasive markers monitoring disease activity in inflammatory bowel disease (IBD). We compared the significance of fecal eosinophil cationic protein (fECP) and fecal calprotectin (fCal). Methods: fECP and fCal were measured in patients with Crohn’s disease (CD, n = 97), ulcerative colitis (UC, n = 53), Clostridioides difficile infection (CDI, n = 9), primary food allergy (PFA, n = 11), pollen-associated food allergy (n = 25) and non-inflammatory controls (n = 78). Results were correlated with clinical and endoscopic IBD activity scores. Results: fECP was significantly elevated in CD, UC, CDI and PFA compared to controls. fCal was significantly increased in CD, UC and CDI. fECP had lower diagnostic accuracy than fCal (area under the curve (AUC) = 0.88) in differentiating between endoscopically active and inactive patients with IBD (AUC = 0.77, ROC analysis). In contrast to fCal, fECP correlated negatively with age and levels were also elevated in clinically and endoscopically inactive patients with IBD <45 years (endoscopically inactive IBD vs controls; AUC for fECP = 0.86; AUC for fCal = 0.62). However, in those patients with low inflammatory activity (fCal <250 mg/kg), high fECP indicated the need for treatment modification or surgery (fECP <200 µg/kg = 22%; 200–600 µg/kg = 44%; >600 µg/kg = 82%) at month 48 of follow-up. Conclusions: fECP is a diagnostic and prognostic marker in young patients with IBD in remission.

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