Der p 1 and Der p 2 induce less severe late asthmatic responses than native Dermatophagoides pteronyssinus extract after a similar early asthmatic response

2001 ◽  
Vol 31 (5) ◽  
pp. 705-714 ◽  
Author(s):  
M. J. Van Der Veen ◽  
H. M. Jansen ◽  
R. C. Aalberse ◽  
J. S. Van Der Zee
Keyword(s):  
Dermatophagoides Pteronyssinus ◽  
Asthmatic Response ◽  
Der P 1 ◽  
Der P 2 ◽  
Early Asthmatic Response

IgE responses to Dermatophagoides pteronyssinus native major allergens Der p 1 and Der p 2 during long-term specific immunotherapy

Allergy ◽  
1997 ◽  
Vol 52 (11) ◽  
pp. 1115-1119 ◽  
Author(s):  
F. Mastrandrea ◽  
G. Serio ◽  
A. Minardi ◽  
G. Coradduzza ◽  
N. Rossi ◽  
...  
Keyword(s):  
Specific Immunotherapy ◽  
Dermatophagoides Pteronyssinus ◽  
Der P 1 ◽  
Major Allergens ◽  
Der P 2

Defection of der p 1 and der p 2 in enzymatically inactive extracts of dermatophagoides pteronyssinus

2003 ◽  
Vol 111 (2) ◽  
pp. S89
Author(s):  
J. Carnés ◽  
M. Gallego ◽  
F. Marañón ◽  
V. Iraola ◽  
E. Fernandez-Caldas
Keyword(s):  
Dermatophagoides Pteronyssinus ◽  
Der P 1 ◽  
Der P 2

Induction of Rabbit Specific IgG against Der p 1 and Der p 2 after Immunization with an Allergoid of Dermatophagoides Pteronyssinus

2006 ◽  
Vol 117 (2) ◽  
pp. S278
Author(s):  
E. Fernández-Caldas ◽  
M. Gallego ◽  
J. Carnés ◽  
M. Casanovas ◽  
V. Iraola
Keyword(s):  
Dermatophagoides Pteronyssinus ◽  
Specific Igg ◽  
Der P 1 ◽  
Der P 2

Serum levels of specific immunoglobulin E to Dermatophagoides pteronyssinus allergen components in patients with allergic rhinitis or/and asthma

2021 ◽  
Vol 42 (1) ◽  
pp. e40-e46 ◽  
Author(s):  
Xiangwei Zou ◽  
Haisheng Hu ◽  
Zhifeng Huang ◽  
Chenxi Liao ◽  
Liuqiao Huang ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Southern China ◽  
Serum Levels ◽  
Dermatophagoides Pteronyssinus ◽  
Allergen Components ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Positive Rate ◽  
Der P 1 ◽  
Der P 2

Background: House-dust mites (HDM) allergen is one of the most important allergens in southern China; however, studies on the Dermatophagoides pteronyssinus components are relatively lacking. Objective: This study analyzed the molecular components of D. pteronyssinus in patients with allergic asthma (AS) and/or allergic rhinitis (AR) sensitized to D. pteronyssinus, and aimed to improve HDM immunotherapy in southern China. Methods: Allergen component-resolved diagnosis detection technology was used to detect the serum levels of specific immunoglobulin E (sIgE) to D. pteronyssinus allergen components (Der p 1, 2, 3, 5, 7, 10, and 23) in patients who were sensitized to D. pteronyssinus and with AR (n = 106), AS (n = 144), or AR combined with AS (n = 134). Results: The highest positive rates of D. pteronyssinus components were Der p 1 (94.8%), followed by Der p 2 (77.6%), Der p 23 (62.5%), Der p 7 (34.6%), Der p 5 (17.7%), Der p 10 (12.2%), and Der p 3 (2.6%). Patients with AR+AS had the highest positive rates to Der p 2 (85.8%), Der p 23 (62.7%), Der p 7 (40.3%), Der p 5 (25.0%), and Der p 10 (16.4%). Der p 1 had the highest positive rate in patients with AR (95.3%). The Der p 3 positive rate in patients with AS (6.0%) was higher than that in patients with AR (0.0%, χ2 = 6.872, p < 0.05) and patients with AR+AS (0.7%, χ2 = 6.063, p < 0.05) Among the patients with AR+AS, 19.1% were co-sensitized to Der p 1, Der p 2, Der p 23, and Der p 7. Interestingly, only one patient with AR was exclusively sensitized to Der p 23. An optimal scale analysis showed that Der p 5, Der p 23, and Der p 7 had strong connection (Cronbach α = 93.7%). Conclusion: Der p 1 and Der p 2 were the main sensitization components of D. pteronyssinus, and patients with AS+AR had the highest positive rate for five of seven D. pteronyssinus allergen components. This research can provide suggestions for personalized HDM-specific immunotherapy in southern China.

scholarly journals An Engineered Hybrid Protein from Dermatophagoides pteronyssinus Allergens Shows Hypoallergenicity

2019 ◽  
Vol 20 (12) ◽  
pp. 3025 ◽  
Author(s):  
Dalgys Martínez ◽  
Marlon Munera ◽  
Jose Fernando Cantillo ◽  
Judith Wortmann ◽  
Josefina Zakzuk ◽  
...  
Keyword(s):  
Cysteine Protease ◽  
Protease Activity ◽  
Dermatophagoides Pteronyssinus ◽  
Basophil Activation ◽  
Allergen Extract ◽  
Hybrid Protein ◽  
Dot Blot ◽  
Cysteine Protease Activity ◽  
Der P 1 ◽  
Der P 2

The house dust mite (HDM) Dermatophagoides pteronyssinus is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that is based on recombinant proteins has been proposed for the safer and more efficient treatment of allergic diseases. The aim of this study was to design and obtain a hybrid protein (DPx4) containing antigenic regions of allergens Der p 1, Der p 2, Der p 7, and Der p 10 from this mite. DPx4 was produced in Escherichia coli and its folding was determined by circular dichroism. Non-denaturing dot-blot, ELISA, basophil activation test, dot blot with monoclonal antibodies, ELISA inhibition, and cysteine protease activity assays were performed. Mice that were immunized with DPx4 were also analyzed. We found that DPx4 had no cysteine protease activity and it showed significantly lower IgE reactivity than Der p 1, Der p 2, and D. pteronyssinus extract. DPx4 induced lower basophil activation than Der p 2 and the allergen extract. Immunized mice produced IgG antibodies that inhibited the binding of allergic patient’s IgE to the allergen extract and induced comparatively higher levels of IL-10 than the extract in peripheral blood mononuclear cells (PBMC) culture. These results suggest that DPx4 has immunological properties that are useful for the development of a mite allergy vaccine.

Recombinant Fusion Proteins Assembling Der p 1 and Der p 2 Allergens from Dermatophagoides pteronyssinus

2010 ◽  
Vol 153 (2) ◽  
pp. 141-151 ◽  
Author(s):  
Laetitia Bussières ◽  
Véronique Bordas-Le Floch ◽  
Ingrid Bulder ◽  
Henri Chabre ◽  
Emmanuel Nony ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Dermatophagoides Pteronyssinus ◽  
Recombinant Fusion Proteins ◽  
Der P 1 ◽  
Der P 2

scholarly journals The IgE Blocking Activity Induced by Dermatophagoides pteronyssinus Subcutaneous Immunotherapy Does Not Correlate with Specific IgA but with IgG4 in both Serum and Saliva

2021 ◽  
pp. 1-14
Author(s):  
Yong He ◽  
Jinling Liu ◽  
Deyu Zhao ◽  
Suqin Zhang ◽  
Guodong Hao ◽  
...  
Keyword(s):  
Treatment Time ◽  
Symptomatic Treatment ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
Subcutaneous Immunotherapy ◽  
Control Group ◽  
Serum Igg4 ◽  
Specific Igg4 ◽  
Blocking Activity ◽  
Der P 2

<b><i>Background:</i></b> The role of salivary-specific IgG4 and IgA in subcutaneous immunotherapy (SCIT) is not well defined. We aimed to investigate the change of IgG4 and IgA in both serum and saliva and their correlations with IgE-blocking-factor (IgE-BF) during SCIT. <b><i>Method:</i></b> 307 <i>Dermatophagoides pteronyssinus</i> (DP) allergic rhinitis and/or asthma patients were recruited for this study. 286 patients received DP-SCIT for 1 year. Twenty-one patients received only symptomatic treatment. DP-, Der p 1-, and Der p 2-specific IgE in serum, specific-IgG4 and Der p 2-specific IgA1 and IgA2 in both serum and saliva were measured at timepoints 0, 4, and 12 months during DP-SCIT. Correlation between salivary and serological IgG4, IgA, and their correlation with DP-specific IgE-BF measured in serum was evaluated. <b><i>Results:</i></b> During DP-SCIT, the allergen-specific IgG4 in both saliva and serum increased and correlated significantly, the correlation becomes stronger over the treatment time. DP-specific IgE-BF significantly correlated with DP-specific IgG4 in serum (<i>p</i> &#x3c; 0.0001) at different timepoints and in saliva at 12 months of SCIT (<i>p</i> &#x3c; 0.01). No change in Der p 2-specific IgA during DP-SCIT was observed, and the IgA in serum did not correlate with IgA in saliva. There was no correlation between DP IgE-BF and Der p 2-specific IgA in serum or saliva. The control group did not exhibit significant changes in any antibody level measured. <b><i>Conclusion:</i></b> The IgE blocking activity induced by DP-SCIT treatment correlated with specific IgG4 and not IgA. The IgG4 in saliva correlates with serum IgG4 and can be an alternative immunological marker beyond 1 year of SCIT treatment.

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