Peripheral nerve regeneration through nerve guides seeded with adult Schwann cells

1997 ◽  
Vol 23 (5) ◽  
pp. 387-398 ◽  
Author(s):  
A.D. Ansselin ◽  
T. Fink ◽  
D.F. Davey
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Nerve Guides

scholarly journals Histopathological analysis of gangliosides use in peripheral nerve regeneration after axonotmesis in rats

2008 ◽  
Vol 23 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Camila Maria Beder Ribeiro ◽  
Belmiro Cavalcanti do Egito Vasconcelos ◽  
Joaquim Celestino da Silva Neto ◽  
Valdemiro Amaro da Silva Júnior ◽  
Nancy Gurgel Figueiredo
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Cell Activity ◽  
Control Group ◽  
Histopathological Analysis ◽  
Male Wistar Rats ◽  
The Right

PURPOSE: To analyze the action of gangliosides in peripheral nerve regeneration in the sciatic nerve of the rat. METHODS: The sample was composed of 96 male Wistar rats. The animals were anaesthetized and, after identification of the anaesthesic plane, an incision was made in the posterior region of the thigh, followed by skin and muscle divulsion. The right sciatic nerve was isolated and compressed for 2 minutes. Continuous suture of the skin was performed. The animals were randomly divided into two groups: the experimental group (EG), which received subcutaneous injection of gangliosides, and the control group (CG), which received saline solution (0.9%) to mimic the effects of drug administration. RESULTS: No differences were observed between the experimental and control groups evaluated on the eighth day of observation. At 15 and 30 days the EG showed an decrease in Schwann cell activity and an apparent improvement in fibre organization; at 60 days, there was a slight presence of Schwann cells in the endoneural space and the fibres were organized, indicating nerve regeneration. At 15 and 30 days, the level of cell reaction in the CG had diminished, but there were many cells with cytoplasm in activity and in mitosis; at 60 days, hyperplastic Schwann cells and mitotic activity were again observed, as well as nerve regeneration, but to a lesser extent than in the EG. CONCLUSION: The administration of exogenous gangliosides seems to improve nerve regeneration.

The Astrocyte Inhibition of Peripheral Nerve Regeneration Is Reversed by Schwann Cells

1994 ◽  
Vol 126 (1) ◽  
pp. 44-60 ◽  
Author(s):  
Véronique Guénard ◽  
Patrick Aebischer ◽  
Richard P. Bunge
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration

scholarly journals The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration

Neuron ◽  
2017 ◽  
Vol 96 (1) ◽  
pp. 98-114.e7 ◽  
Author(s):  
Melanie P. Clements ◽  
Elizabeth Byrne ◽  
Luis F. Camarillo Guerrero ◽  
Anne-Laure Cattin ◽  
Leila Zakka ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration

Producing Neurospheroids and Hydrogels to Create a Three-dimensional in Vitro Model for the Use of Conduits in Peripheral Nerve Regeneration

Neurosurgery ◽  
2019 ◽  
Vol 84 (5) ◽  
pp. E272-E272
Author(s):  
Devyani Shete ◽  
Aran Batth ◽  
Aditi Nijhawan ◽  
Jaffer Choudhary ◽  
Ian Thompson
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
3D Structure ◽  
Three Dimensional ◽  
Negative Control ◽  
Cellular Growth ◽  
Live Cells

Abstract INTRODUCTION Peripheral nerve regeneration is a complex challenge that requires suitable nerve guidance systems to bridge the severed ends of 2 nerves back together. Current polymeric conduits on the market provide good cellular growth but are limited by the length of gap defect they can repair, and complete functional recovery is rare. This project focused on creating a three-dimensional (3D) in Vitro spheroidal sprouting assay for peripheral nerve regeneration, as well as producing and testing different polymeric hydrogels as potential scaffold materials for the conduit. METHODS Different concentrations of chitosan, methylcellulose (MC) and sodium alginate were produced, as well as blends of these materials. These hydrogels were seeded with 3D neurospheroids, along with NG108-15 (neuronal) cells and Schwann cells to test their biocompatibility. RESULTS MTT assays showed the mean absorbance of chitosan gels with NG108-15 cells at 24 hr (P < .001) and 72 hr (P > .05) was similar/slightly higher than the negative control. Live-Dead data showed 93.4% of live cells at DIV7 on MC: Ch blends, compared to 72% with chitosan alone. CONCLUSION Overall, both chitosan and MC were nontoxic and biocompatible with NG108-15 and Schwann cells. Blending chitosan with MC improved its chemical and physical properties. The cells formed spheroids that well on a gel; this pseudo-3D structure is excellent for research purposes compared to 2D as it mimics the body's internal environment.

PERIPHERAL NERVE REGENERATION THROUGH NERVE GUIDES FILLED WITH BILOBALIDE AND SCHWANN CELLS

2006 ◽  
Vol 18 (01) ◽  
pp. 8-12
Author(s):  
MING-CHIN LU ◽  
YUNG-HISEN CHANG ◽  
LEIH-CHIH CHIANG ◽  
HAI-TING WANG ◽  
CHUN-YUAN CHENG ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Chinese Medicine ◽  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
New Approach ◽  
Nerve Guides ◽  
Growth Promoting

The present study provides in vivo trials of silicone rubber chambers filled with different concentrations of bilobalide (0, 50, 100, 200, 400 μM) and Schwann cells (1.5 × 105 cell/ml) in a 1:1 volumetric addition to bridge a 15 mm sciatic nerve defect in rats. At the conclusion of 8 weeks, histological technique was used to evaluate the functional recovery of the nerve. In the groups receiving the Schwann cells and bilobalide at 50, 100, 200 and 400 μM, 44% (4 of 9, one died during experiment), 50% (5 of 10), 30% (3 of 10), and 60% (6 of 10) of the animals exhibiting a regenerated nerve cable across the 15-mm gap, respectively. In comparison, 50% (5 of 10) of the animals in the group with Schwann cells only showed such regenerated nerve cables. Although the adding of bilobalide did not promote the nerve growth-promoting capability of Schwann cells in the nerve guides, the techniques we used in this study provided a new approach combining Chinese medicine and tissue engineering to nerve regeneration.

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