Ratio-imaging of Ca2+i in the self-incompatibility response in pollen tubes of Papaver rhoeas

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

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Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

The Plant Cell ◽

10.1105/tpc.3.3.271 ◽

1991 ◽

pp. 271-283 ◽

Cited By ~ 66

Author(s):

J. E. Gray ◽

B. A. McClure ◽

I. Bonig ◽

M. A. Anderson ◽

A. E. Clarke

Keyword(s):

Pollen Tubes ◽

The Self ◽

Nicotiana Alata ◽

Self Incompatibility

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The population genetics of the self-incompatibility polymorphism in Papaver rhoeas. II. The number and frequency of S-alleles in a natural population (R106)

Heredity ◽

10.1038/hdy.1981.8 ◽

1981 ◽

Vol 46 (1) ◽

pp. 81-90 ◽

Cited By ~ 41

Author(s):

J M Campbell ◽

M J Lawrence

Keyword(s):

Population Genetics ◽

Natural Population ◽

The Self ◽

Self Incompatibility ◽

Papaver Rhoeas ◽

S Alleles

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Incompatibility Alleles in Portuguese Hazelnut Landraces

ISRN Agronomy ◽

10.5402/2012/154723 ◽

2012 ◽

Vol 2012 ◽

pp. 1-6

Author(s):

Sandra Martins ◽

Mercè Rovira ◽

Ana Paula Silva ◽

Valdemar Carnide

Keyword(s):

Higher Plants ◽

Pollen Tubes ◽

The Self ◽

Single Locus ◽

Self Incompatibility ◽

Multiple Alleles ◽

Breeding Programmes ◽

Incompatibility Alleles ◽

Related Individuals ◽

New Cultivars

In many higher plants, selffertilization and genetically related individuals are prevented by pollen-stigma incompatibility. In the genus Corylus, incompatibility is of the sporophytic type and controlled by a single locus with multiple alleles. The objective of this study is to identify the S-alleles present in a collection of Portuguese landraces in order to select the most appropriate landraces for establishment of future orchards and for breeding programmes. Ten major Portuguese hazelnut landraces were submitted to controlled pollinations in the field, with 18 genotypes whose S-alleles are known. The pollen tubes were observed at 100X under a florescence microscope to evaluate their development. Three landraces were revealed to have S2 allele, two have S5, and four have one of the S3, S5, S10, and S18 alleles. One landrace was compatible with the 18 S-alleles tested and for two landraces, it was possible to identify both alleles. The information of the self-incompatibility relationship between these old cultivars is obviously useful for selecting the most suitable pollinators for planning new orchards and for new cultivars development.

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