Alterations in the Actin Cytoskeleton of Pollen Tubes Are Induced by the Self-Incompatibility Reaction in Papaver rhoeas

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

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Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes.

The Plant Cell ◽

10.1105/tpc.3.3.271 ◽

1991 ◽

pp. 271-283 ◽

Cited By ~ 66

Author(s):

J. E. Gray ◽

B. A. McClure ◽

I. Bonig ◽

M. A. Anderson ◽

A. E. Clarke

Keyword(s):

Pollen Tubes ◽

The Self ◽

Nicotiana Alata ◽

Self Incompatibility

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The population genetics of the self-incompatibility polymorphism in Papaver rhoeas. II. The number and frequency of S-alleles in a natural population (R106)

Heredity ◽

10.1038/hdy.1981.8 ◽

1981 ◽

Vol 46 (1) ◽

pp. 81-90 ◽

Cited By ~ 41

Author(s):

J M Campbell ◽

M J Lawrence

Keyword(s):

Population Genetics ◽

Natural Population ◽

The Self ◽

Self Incompatibility ◽

Papaver Rhoeas ◽

S Alleles

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Unilateral incompatibility and the effects of interspecific pollination for Erythronium americanum and Erythronium albidum (Liliaceae)

Canadian Journal of Botany ◽

10.1139/b93-038 ◽

1993 ◽

Vol 71 (2) ◽

pp. 353-358 ◽

Cited By ~ 41

Author(s):

Lawrence D. Harder ◽

Mitchell B. Cruzan ◽

James D. Thomson

Keyword(s):

Seed Production ◽

Pollen Tubes ◽

The Self ◽

Pollen Transfer ◽

Interspecific Incompatibility ◽

Unilateral Incompatibility ◽

Heterospecific Pollen ◽

Interspecific Pollen Transfer ◽

Erythronium Americanum ◽

Incompatibility Reaction

To determine whether interspecific pollen transfer could reduce seed production by two sympatric lilies, Erythronium albidum and Erythronium americanum, we hand-pollinated flowers with mixtures of conspecific and heterospecific pollen. These species exhibited typical unilateral interspecific incompatibility, i.e., pollen tubes from the self-infertile species (E. americanum) grew apparently unimpeded in styles of the self-fertile species (E. albidum), whereas the reverse cross resulted in an incompatibility reaction. Because of this asymmetrical relation and faster growth by heterospecific pollen tubes in E. albidum stigmas than by conspecific tubes, pollination with abundant heterospecific pollen reduced fruit and seed production by E. albidum, but not by E. americanum, as long as the stigma also received some conspecific pollen. Unilateral incompatibility could benefit self-infertile species in reproductive interactions with closely related self-fertile species; however, this benefit remains to be demonstrated for naturally pollinated plants. Key words: Erythronium albidum, Erythronium americanum, interspecific pollen transfer, pollination, unilateral incompatibility.

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