The effects of long-term exposure to simulated acid rain on the development of pine wilt disease caused by Bursaphelenchus xylophilus

Pine wilt disease, caused by the pinewood nematode Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle, is originating severe infections in pine trees. The disease is detected when external symptoms appear (e.g. needle chlorosis), but trees could remain asymptomatic for long periods and serve as a long-term host. The primary goal of this study was to assess the effect of inoculation with an avirulent isolate of B. xylophilus (C14-5) on different Pinus spp. seedlings (P. sylvestris, P. nigra, P. pinea and P. pinaster). At the same time, seedlings were also inoculated with a virulent strain, HF, in order to compare the phenotypic and genomic results of the two types of inoculations. The effect of inoculation was determined in terms of expression of various Pinus genes potentially involved in the response to the disease.The results suggest that P. pinea and P. nigra are more resistant to infection by the nematode than P. sylvestris and P. pinaster. The phenotypic and genetic differences were more marked among P. pinea and P. pinaster.

Download Full-text

Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease?

Forest Pathology ◽

10.1111/efp.12564 ◽

2019 ◽

Vol 49 (6) ◽

pp. e12564

Author(s):

Marta Salgueiro Alves ◽

Anabela Pereira ◽

Cláudia Vicente ◽

Manuel Mota ◽

Isabel Henriques

Keyword(s):

Pine Wilt Disease ◽

Bursaphelenchus Xylophilus ◽

Wilt Disease ◽

Host Tree ◽

Insect Vector ◽

Pine Wilt

Download Full-text

Volatile compounds in pine stands suffering from pine wilt disease: qualitative and quantitative evaluation

Nematology ◽

10.1163/156854106779799295 ◽

2006 ◽

Vol 8 (6) ◽

pp. 869-879 ◽

Cited By ~ 10

Author(s):

Kazuyoshi Futai ◽

Natsumi Kanzaki ◽

Yuko Takeuchi

Keyword(s):

Volatile Compounds ◽

Pine Wilt Disease ◽

Bursaphelenchus Xylophilus ◽

Wilt Disease ◽

Economic Damage ◽

Qualitative And Quantitative ◽

Pine Wilt ◽

Pine Trees ◽

Quantitative Properties ◽

Pine Stands

AbstractPine wilt disease causes ecological and economic damage in Japanese pine forests in spite of intensive effort to protect them from the pine wood nematode, Bursaphelenchus xylophilus. Pine trees infected with B. xylophilus emit a characteristic bouquet of volatile compounds bioactive to the vector beetle of the nematode, Monochamus alternatus, and potentially affecting symptom development inside the trees. To investigate the qualitative and quantitative properties of volatile compounds in the field, we profiled the volatile emissions in two Japanese black pine stands, one naturally suffering from pine wilt disease and the other artificially inoculated with B. xylophilus. In both pine stands, the emission of some terpenoids from the infected trees such as (−)-α-pinene, began to increase in summer, overlapping the oviposition season of the vector beetle, but peaked in the summer and autumn. These data suggest that the beetles may not necessarily depend on the tremendous quantity of volatiles alone when they search for suitable trees on which to oviposit.

Download Full-text

Cloning arginine kinase gene and its RNAi in Bursaphelenchus xylophilus causing pine wilt disease

European Journal of Plant Pathology ◽

10.1007/s10658-012-0035-0 ◽

2012 ◽

Vol 134 (3) ◽

pp. 521-532 ◽

Cited By ~ 6

Author(s):

Xin-rong Wang ◽

Xi Cheng ◽

Ya-dong Li ◽

Jin-ai Zhang ◽

Zhi-fen Zhang ◽

...

Keyword(s):

Arginine Kinase ◽

Pine Wilt Disease ◽

Bursaphelenchus Xylophilus ◽

Wilt Disease ◽

Kinase Gene ◽

Pine Wilt

Download Full-text

Monochamus galloprovincialis and Bursaphelenchus xylophilus life history in an area severely affected by pine wilt disease: Implications for forest management

Forest Ecology and Management ◽

10.1016/j.foreco.2016.12.027 ◽

2017 ◽

Vol 389 ◽

pp. 105-115 ◽

Cited By ~ 6

Author(s):

Paulo N. Firmino ◽

Teresa Calvão ◽

Matthew P. Ayres ◽

Carla S. Pimentel

Keyword(s):

Life History ◽

Forest Management ◽

Pine Wilt Disease ◽

Bursaphelenchus Xylophilus ◽

Wilt Disease ◽

Monochamus Galloprovincialis ◽

Pine Wilt

Download Full-text

Inhibition response of Pinus densiflora clones to Bursaphelenchus xylophilus systemic dispersal and their resistance to pine wilt disease

Nematology ◽

10.1163/138855410x535732 ◽

2011 ◽

Vol 13 (6) ◽

pp. 653-659 ◽

Cited By ~ 6

Author(s):

Katsumi Togashi ◽

Hiroko Maezono ◽

Koji Matsunaga ◽

Satoshi Tamaki

Keyword(s):

Pinus Densiflora ◽

Resistance Mechanism ◽

Pine Wilt Disease ◽

Bursaphelenchus Xylophilus ◽

Wilt Disease ◽

Pine Wilt ◽

Inhibition Response ◽

The Mean ◽

The Relationship ◽

Clone Group

AbstractTo determine the relationship between resistance to pine wilt disease and the inhibition of nematode systemic dispersal in Pinus densiflora, a suspension of 200 Bursaphelenchus xylophilus was placed on the upper cut end of 5-cm-long, living or boiled branch sections of 17 clones of pine that had different resistance levels. Significantly more nematodes passed through boiled sections than living sections during 24 h. Living branches of the resistant P. densiflora clone group significantly suppressed the dispersal of B. xylophilus compared with those of the susceptible group, suggesting that the inhibition of nematode systemic dispersal was involved in the resistance mechanism of selected disease-resistant pine clones. However, there was no significant correlation between the resistance class and the mean number of nematodes passing through live branch sections within the resistant clone group. The reason for the lack of correlation is discussed in relation with the resistance mechanism.

Download Full-text