scholarly journals Pseudomonas associated with Bursaphelenchus xylophilus, its insect vector and the host tree: A role in pine wilt disease?

2019 ◽  
Vol 49 (6) ◽  
pp. e12564
Author(s):  
Marta Salgueiro Alves ◽  
Anabela Pereira ◽  
Cláudia Vicente ◽  
Manuel Mota ◽  
Isabel Henriques
Keyword(s):  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Host Tree ◽  
Insect Vector ◽  
Pine Wilt

scholarly journals Insights into the Role of Fungi in Pine Wilt Disease

2021 ◽  
Vol 7 (9) ◽  
pp. 780
Author(s):  
Cláudia S. L. Vicente ◽  
Miguel Soares ◽  
Jorge M. S. Faria ◽  
Ana P. Ramos ◽  
Maria L. Inácio
Keyword(s):  
Complex Disease ◽  
Pine Wilt Disease ◽  
Wilt Disease ◽  
Host Tree ◽  
Fungal Communities ◽  
Insect Vector ◽  
Pinewood Nematode ◽  
Management Actions ◽  
Pine Wilt ◽  
Pinus Spp

Pine wilt disease (PWD) is a complex disease that severely affects the biodiversity and economy of Eurasian coniferous forests. Three factors are described as the main elements of the disease: the pinewood nematode (PWN) Bursaphelenchus xylophilus, the insect-vector Monochamus spp., and the host tree, mainly Pinus spp. Nonetheless, other microbial interactors have also been considered. The study of mycoflora in PWD dates back the late seventies. Culturomic studies have revealed diverse fungal communities associated with all PWD key players, composed frequently of saprophytic fungi (i.e., Aspergillus, Fusarium, Trichoderma) but also of necrotrophic pathogens associated with bark beetles, such as ophiostomatoid or blue-stain fungi. In particular, the ophiostomatoid fungi often recovered from wilted pine trees or insect pupal chambers/tunnels, are considered crucial for nematode multiplication and distribution in the host tree. Naturally occurring mycoflora, reported as possible biocontrol agents of the nematode, are also discussed in this review. This review discloses the contrasting effects of fungal communities in PWD and highlights promising fungal species as sources of PWD biocontrol in the framework of sustainable pest management actions.

scholarly journals First Report of Pine Wilt Disease Caused by Bursaphelenchus xylophilus on Pinus thunbergii in the Inland City of Zibo, Shandong, China

Plant Disease ◽  
2013 ◽  
Vol 97 (8) ◽  
pp. 1126-1126 ◽  
Author(s):  
H. Y. Wu ◽  
Q. Q. Tan ◽  
S. X. Jiang
Keyword(s):  
Body Length ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Shandong Province ◽  
Jiangsu Province ◽  
Insect Vector ◽  
Pinewood Nematode ◽  
Pine Wilt ◽  
Stylet Length

The pinewood nematode (PWN) Bursaphelenchus xylophilus (Steiner & Buhrer 1934) Nickle 1970 is the causal agent of pine wilt disease. It is especially damaging in East Asian countries, including Japan, China, and Korea. In China, the nematode has been found in Anhui, Guangdoung, Guizhou, Chongqing, and Zhejiang Provinces since its discovery in Jiangsu Province in 1982 (1). China is confronted with an enormous threat to its pine forests. B. xylophilus is transmitted by the insect vector pine sawyer beetle (Monochamus alternatus). The main host trees are Pinus massoniana, P. thunbergii, and P. densiflora, which are the most common pine trees in China. Shandong Province, located north of Jiangsu Province, is a high-risk area because it was thought to be the northernmost suitable area for the pine wood nematode. P. tabulaeformis, P. densiflora, and P. thunbergii are the principal hosts. In 2010, a pine tree with suspected wilt disease was found in Lushang Forest (36°16′31.11″ N, 118°03′59.79″ E) of P. thunbergii located in Zibo city of Shandong Province. Symptoms were systemic, with almost all leaves brown or yellowish; the tree was nearly dead. Wood samples were collected and nematodes were extracted using a modified Baermann's funnel method. After 12 h, the nematodes were collected from the wood chips, and their morphology was observed with an inverted light microscope (Nikon 90i, Japan). Nematodes had a typical Aphelenchoid-type esophagus and female vulva flap. Females had subcylindrical tails, usually with broadly rounded terminus, some with a short mucro, and flat vulva, whereas males had large paired arcuate spicules with a sharply pointed prominent rostrum, and typical disc-like expansions on distal ends. Standard measurements of these nematodes were as follows: 25 females: body length = 960.9 ± 117.4 (791.5 to 1,265.2) μm, a = 32.1 ± 5.1 (23.7 to 44.5), b = 13.6 ± 1.4 (11.4 to 16.1), c = 28.3 ± 4.6 (21.7 to 42.2), V = 77.8 ± 2.0 (74.2 to 83.9), stylet length = 13.7 ± 1.6 (11.4 to 17.6) μm; 21 males: body length = 785.6 ± 103.2 (609.6 to 1,004.5) μm, a = 33.3 ± 4.4 (26.0 to 40.8), b = 11.9 ± 1.3 (9.0 to 14.6), c = 31.0 ± 2.7 (25.5 to 37.1), stylet length = 13.5 ± 1.9 (11.0 to 17.5) μm, spicule length = 18.8 ± 2.5 (14.9 to 23.9) μm. The morphometrics of this population, apart from body length and “a” value, which are shorter than the Portugal isolate measured by Mota et al. (3), are very much in the same range reported for B. xylophilus. For a more accurate identification, DNA was extracted from individual nematodes using a liquid nitrogen method. The internal transcribed spacers (ITS-1, ITS-2, 5.8S) were amplified by using PCR (2). Nucleotide sequences were compared with the sequences of B. xylophilus in GenBank, accession nos. JN684828 (Portugal), JN684829 (Portugal), JF826219 (Madeira Island) and JQ288086 (Japan). The ITS DNA sequences of the nematode from P. thunbergii were 99% identical to those of B. xylophilus in GenBank. A sequence of this nematode was submitted to the GenBank database and assigned the number KC460340. We have thus confirmed that B. xylophilus is now present north of Changjiang River in Zibo city, Shandong Province. This range expansion, perhaps the result of global warming, will affect both domestic and international quarantine efforts to control the further spread of pinewood nematode. References: (1) X. Y. Cheng et al. Heredity 100:356, 2008. (2) K. Metge and W. Burgermeister. J. Plant Dis. Protect. 113:275, 2006. (3) M. Mota et al. Nematology 1:727, 1999.

scholarly journals Effects of Several Chemicals on the Migration Behavior of Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle

Forests ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 771
Author(s):  
Xiu-Feng Shi ◽  
Xiao-Lei Ding ◽  
Li-Na Shi ◽  
Qing-Tong Wang ◽  
Jian-Ren Ye
Keyword(s):  
Pine Wilt Disease ◽  
Propagation Rate ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Movement Behavior ◽  
Insect Vector ◽  
Migration Behavior ◽  
Pine Wood Nematode ◽  
Pine Wood ◽  
Pine Wilt

The pine wood nematode (PWN), Bursaphelenchus xylophilus, that causes pine wilt disease (PWD), has severely damaged forests and ecosystems in countries of East Asia and Europe. Since the high propagation rate of PWD is the main cause of the devastating disease, it is very important to interfere with the spread of PWN mediated by its insect vector (Monochamus alternatus). In order to explore new methods and approaches for the prevention and control of pine wood nematode disease, chemicals with attraction or repellency to pine wood nematode and stable diffusion or volatilization in infected wood were screened out to interfere and hinder the pupa chamber effect, the aggregation behavior of PWN to pupa chamber in M. alternatus. In the present study, 22 chemicals were inoculated on water agar medium and infected wood to verify the tropism of PWN toward the chemicals from the aspects of attraction and repellency. The results indicate that linoleic acid, oleic acid, diacetyl and β-myrcene have attractive effects on PWN, while kerosene and β-humulene have repellent effects on PWN and are able to stably permeate and volatilize within pine trees. This research shows that some chemicals can affect the movement behavior of PWN and interfere with its migration to the pupal chamber in M. alternatus, thus reducing the dispersal efficiency of PWNs carried by insect vectors. This chemically based strategy would provide novel approaches for effective controlling of pine wilt disease.

Community and functional diversity of bacteria associated with propagative and dispersal forms of Bursaphelenchus xylophilus

Nematology ◽  
2016 ◽  
Vol 18 (10) ◽  
pp. 1185-1198 ◽  
Author(s):  
Xiao-Qin Wu ◽  
Qi Xue ◽  
Yang Xiang ◽  
Xiao-Lei Ding ◽  
Xiao-Lu Xu ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Total Carbon ◽  
Insect Vector ◽  
Pine Wood Nematode ◽  
Pine Wood ◽  
Pine Wilt

The pine wood nematode (PWN),Bursaphelenchus xylophilus, the pathogen of pine wilt disease, has propagative and dispersal forms in its life cycle, which is associated with the insect vectorMonochamus alternatus. Pine wood nematode-associated bacteria could play important roles in pine wilt disease. In this study, the diversity and population dynamics of bacteria in propagative and dispersal stages of PWN were analysed using cultural and Biolog Eco™ methods coupled with high-throughput sequencing. The results revealed that culturableStenotrophomonasin PWN from diseased pine was not found fromM. alternatus. Bacteria in dispersal PWN carried byM. alternatusshowed the highest total carbon utilisation. The high-throughput analysis showed thatStenotrophomonas,AchromobacterandSphingobacteriumwere more abundant in propagative PWN than in dispersal PWN, whereasCitrobacter,Vibrioand Enterobacteriaceae were more abundant in dispersal PWN. The results showed that bacteria of PWN in different developmental forms differed. This change of bacteria might help PWN to adapt to the changing environment.

scholarly journals Effects of cavitation on the development of pine wilt disease caused by Bursaphelenchus xylophilus.

1988 ◽  
Vol 54 (5) ◽  
pp. 606-615 ◽  
Author(s):  
Keiko KURODA ◽  
Toshihiro YAMADA ◽  
Kazuhiko MINEO ◽  
Hirotada TAMURA
Keyword(s):  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Pine Wilt

Volatile compounds in pine stands suffering from pine wilt disease: qualitative and quantitative evaluation

Nematology ◽  
2006 ◽  
Vol 8 (6) ◽  
pp. 869-879 ◽  
Author(s):  
Kazuyoshi Futai ◽  
Natsumi Kanzaki ◽  
Yuko Takeuchi
Keyword(s):  
Volatile Compounds ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Economic Damage ◽  
Qualitative And Quantitative ◽  
Pine Wilt ◽  
Pine Trees ◽  
Quantitative Properties ◽  
Pine Stands

AbstractPine wilt disease causes ecological and economic damage in Japanese pine forests in spite of intensive effort to protect them from the pine wood nematode, Bursaphelenchus xylophilus. Pine trees infected with B. xylophilus emit a characteristic bouquet of volatile compounds bioactive to the vector beetle of the nematode, Monochamus alternatus, and potentially affecting symptom development inside the trees. To investigate the qualitative and quantitative properties of volatile compounds in the field, we profiled the volatile emissions in two Japanese black pine stands, one naturally suffering from pine wilt disease and the other artificially inoculated with B. xylophilus. In both pine stands, the emission of some terpenoids from the infected trees such as (−)-α-pinene, began to increase in summer, overlapping the oviposition season of the vector beetle, but peaked in the summer and autumn. These data suggest that the beetles may not necessarily depend on the tremendous quantity of volatiles alone when they search for suitable trees on which to oviposit.

Cloning arginine kinase gene and its RNAi in Bursaphelenchus xylophilus causing pine wilt disease

2012 ◽  
Vol 134 (3) ◽  
pp. 521-532 ◽  
Author(s):  
Xin-rong Wang ◽  
Xi Cheng ◽  
Ya-dong Li ◽  
Jin-ai Zhang ◽  
Zhi-fen Zhang ◽  
...  
Keyword(s):  
Arginine Kinase ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Kinase Gene ◽  
Pine Wilt

Inhibition response of Pinus densiflora clones to Bursaphelenchus xylophilus systemic dispersal and their resistance to pine wilt disease

Nematology ◽  
2011 ◽  
Vol 13 (6) ◽  
pp. 653-659 ◽  
Author(s):  
Katsumi Togashi ◽  
Hiroko Maezono ◽  
Koji Matsunaga ◽  
Satoshi Tamaki
Keyword(s):  
Pinus Densiflora ◽  
Resistance Mechanism ◽  
Pine Wilt Disease ◽  
Bursaphelenchus Xylophilus ◽  
Wilt Disease ◽  
Pine Wilt ◽  
Inhibition Response ◽  
The Mean ◽  
The Relationship ◽  
Clone Group

AbstractTo determine the relationship between resistance to pine wilt disease and the inhibition of nematode systemic dispersal in Pinus densiflora, a suspension of 200 Bursaphelenchus xylophilus was placed on the upper cut end of 5-cm-long, living or boiled branch sections of 17 clones of pine that had different resistance levels. Significantly more nematodes passed through boiled sections than living sections during 24 h. Living branches of the resistant P. densiflora clone group significantly suppressed the dispersal of B. xylophilus compared with those of the susceptible group, suggesting that the inhibition of nematode systemic dispersal was involved in the resistance mechanism of selected disease-resistant pine clones. However, there was no significant correlation between the resistance class and the mean number of nematodes passing through live branch sections within the resistant clone group. The reason for the lack of correlation is discussed in relation with the resistance mechanism.

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