Fibronectin-binding protein A of Staphylococcus aureus has multiple, substituting, binding regions that mediate adherence to fibronectin and invasion of endothelial cells

2001 ◽  
Vol 3 (12) ◽  
pp. 839-851 ◽  
Author(s):  
Ruth C. Massey ◽  
Maria N. Kantzanou ◽  
Trent Fowler ◽  
Nicholas P. J. Day ◽  
Karin Schofield ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Endothelial Cells ◽  
Binding Protein ◽  
Protein A ◽  
Binding Regions ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

Adherence of Staphylococcus aureus fibronectin binding protein A mutants: an investigation using optical tweezers

2004 ◽  
Vol 21 (3-5) ◽  
pp. 105-111 ◽  
Author(s):  
Kathryn H. Simpson ◽  
M. Gabriela Bowden ◽  
Sharon J. Peacock ◽  
Maneesh Arya ◽  
Magnus Höök ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Optical Tweezers ◽  
Binding Protein ◽  
Protein A ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

scholarly journals Fibrinogen and elastin bind to the same region within the A domain of fibronectin binding protein A, an MSCRAMM of Staphylococcus aureus

2007 ◽  
Vol 63 (3) ◽  
Author(s):  
Fiona M. Keane ◽  
Anthony Loughman ◽  
Viviana Valtulina ◽  
Marian Brennan ◽  
Pietro Speziale ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Protein A ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

scholarly journals Polymorphisms in fibronectin binding protein A of Staphylococcus aureus are associated with infection of cardiovascular devices

2011 ◽  
Vol 108 (45) ◽  
pp. 18372-18377 ◽  
Author(s):  
S. K. Lower ◽  
S. Lamlertthon ◽  
N. N. Casillas-Ituarte ◽  
R. D. Lins ◽  
R. Yongsunthon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Protein A ◽  
Cardiovascular Devices ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

Contribution of (sub)domains of Staphylococcus aureus fibronectin-binding protein to the proinflammatory and procoagulant response of human vascular endothelial cells

2009 ◽  
Vol 101 (03) ◽  
pp. 495-504 ◽  
Author(s):  
Ruth Heying ◽  
Joke van de Gevel ◽  
Yok-Ai Que ◽  
Lionel Piroth ◽  
Philippe Moreillon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Binding Protein ◽  
Protein A ◽  
Surface Expression ◽  
Vascular Endothelial ◽  
Non Invasive ◽  
Fibronectin Binding Protein

SummaryThe Staphylococcus aureus fibronectin (Fn) -binding protein A (FnBPA) is involved in bacterium-endothelium interactions which is one of the crucial events leading to infective endocarditis (IE). We previously showed that the sole expression of S. aureus FnBPA was sufficient to confer to non-invasive Lactococcus lactis bacteria the capacity to invade human endothelial cells (ECs) and to launch the typical endothelial proinflammatory and procoagulant responses that characterize IE. In the present study we further questioned whether these bacterium-EC interactions could be reproduced by single or combined FnBPA subdomains (A, B, C or D) using a large library of truncated FnBPA constructs expressed in L. lactis. Significant invasion of cultured ECs was found for L. lactis expressing the FnBPA subdomains CD (aa 604–877) or A4+16 (aa 432–559). Moreover, this correlates with the capacity of these fragments to elicit in vitro a marked increase in EC surface expression of both ICAM-1 and VCAM-1 and secretion of the CXCL8 chemokine and finally to induce a tissue factor-dependent endothelial coagulation response. We thus conclude that (sub)domains of the staphylococcal FnBPA molecule that express Fn-binding modules, alone or in combination, are sufficient to evoke an endothelial proinflammatory as well as a procoagulant response and thus account for IE severity.

scholarly journals Identification of the Immunodominant Regions of Staphylococcus aureus Fibronectin-Binding Protein A

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e95338 ◽  
Author(s):  
Qian-Fei Zuo ◽  
Chang-Zhi Cai ◽  
Hong-Lei Ding ◽  
Yi Wu ◽  
Liu-Yang Yang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Binding Protein ◽  
Protein A ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding
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