scholarly journals Effects of Human Type 1α Metabotropic Glutamate Receptor Expression Level on Phosphoinositide and Ca2+ Signalling in an Inducible Cell Expression System

2002 ◽  
Vol 70 (4) ◽  
pp. 1772-1775 ◽  
Author(s):  
E. Hermans ◽  
K. W. Young ◽  
R. A. J. Challiss ◽  
S. R. Nahorski
Keyword(s):  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Expression System ◽  
Receptor Expression ◽  
Expression Level ◽  
Metabotropic Glutamate ◽  
Human Type ◽  
Cell Expression ◽  
Cell Expression System

TRPC1 and metabotropic glutamate receptor expression in rat auditory midbrain neurons

2015 ◽  
Vol 93 (6) ◽  
pp. 964-972 ◽  
Author(s):  
Maria Ll. Valero ◽  
Elena Caminos ◽  
Jose M. Juiz ◽  
Juan R. Martinez-Galan
Keyword(s):  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Receptor Expression ◽  
Auditory Midbrain ◽  
Metabotropic Glutamate ◽  
Midbrain Neurons

Ecdysone-Based System for Controlled Inducible Expression of Metabotropic Glutamate Receptor Subtypes 2, 5, and 8

2005 ◽  
Vol 10 (8) ◽  
pp. 841-848 ◽  
Author(s):  
Patrick. M. Downey ◽  
Gianluca Lozza ◽  
Roberta Petrò ◽  
Enrica Diodato ◽  
Chiara Foglia ◽  
...  
Keyword(s):  
G Proteins ◽  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Inducible Expression ◽  
Receptor Expression ◽  
Hek293 Cells ◽  
Receptor Subtypes ◽  
Metabotropic Glutamate ◽  
Recombinant Receptors ◽  
Combined Use

Stable and inducible expression of human metabotropic glutamate receptor types 2, 5, and 8 was achieved in HEK293 cells using the ecdysone inducible system. Treatment of the respective cell lines with ponasterone A resulted in time and concentration-dependent induction of receptor expression. In all cases, the functional activation of receptorswas determined bymeasuring increases in intracellular calcium. The physiologically G• i-coupled receptors mGluR2 andm GluR8 were successfully coupled to phospholipase Cactivation using the chimeric Gprotein G• q/o. The pharmacological properties of recombinant receptors were characterized and proved to be similar to native receptors. Our data suggest that the ecdysone system has a number of characteristics thatmake itwell suited for expressing mGluRs and that the combined use of this systemand chimeric G proteins allows receptors to be characterized using a rapid and straightforward Ca 2+assay.

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