Sampling using horizontal directional drilling allows the collection of soil samples from suspected zones located beneath structures and other surface-subsurface obstacles. However, current horizontal sampling techniques are time consuming and thus expensive. This paper describes the design and testing of a new multiple-port sampler for collection of soil samples from directionally drilled boreholes. The device uses a new methodology for horizontal sampling to improve cost-effectiveness and sample quality.Key words: horizontal directional drilling, horizontal wells, design, remediation, site characterization, sampling.