Systematic toxicological analysis of Indian herbal ready-to-chew pouches by gas chromatography mass spectrometry

AbstractThe so-called systematic toxicological analysis (STA) aiming at simultaneous analysis of as many toxicologically relevant compounds in biosamples as possible is an important part of routine analysis in clinical and forensic toxicology. Gas chromatography-mass spectrometry and liquid chromatography with diode array detection have been the most widely used techniques for this purpose. However, in recent years STA methods based on liquid chromatography coupled to mass spectrometry (LC-MS) or tandem mass spectrometry (LC-MS/MS) have become increasingly important, although their widespread use is still hampered by the lack of a universal reference library of mass spectra that can be used on all major instrument platforms. In this review, LC-MS(/MS) methods for STA in urine and/or blood published in the past 6 years will be compared and discussed with regard to sample preparation, separation, instrument types used for mass spectrometric detection, and method validation. In addition, different approaches to achieving the goal of a universal reference library will be summarized.

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Determination of clozapine, and five antidepressants in human plasma, serum and whole blood by gas chromatography–mass spectrometry: A simple tool for clinical and postmortem toxicological analysis

Journal of Chromatography B ◽

10.1016/j.jchromb.2016.10.023 ◽

2016 ◽

Vol 1038 ◽

pp. 43-48 ◽

Cited By ~ 20

Author(s):

Vassiliki A. Boumba ◽

George Rallis ◽

Petros Petrikis ◽

Theodore Vougiouklakis ◽

Venetsanos Mavreas

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Human Plasma ◽

Whole Blood ◽

Gas Chromatography Mass Spectrometry ◽

Toxicological Analysis ◽

Simple Tool ◽

Chromatography Mass Spectrometry

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Severe isopropanolemia without acetonemia or clinical manifestations of isopropanol intoxication

Clinical Chemistry ◽

10.1093/clinchem/39.9.1922 ◽

1993 ◽

Vol 39 (9) ◽

pp. 1922-1925 ◽

Cited By ~ 13

Author(s):

K M Chan ◽

E T Wong ◽

W S Matthews

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Serum Concentration ◽

Half Life ◽

Clinical Manifestations ◽

Gas Chromatography Mass Spectrometry ◽

Toxicological Analysis ◽

Chromatography Mass Spectrometry ◽

Osmolal Gap

Abstract This is the first reported case of severe isopropanolemia in a patient who did not experience associated clinical manifestations and acetonemia. The patient was found lying face down in a hotel lobby but at admission was alert and oriented to place and person. Toxicological analysis of the patient's serum revealed the presence of isopropanol at a concentration of 72 mmol/L. An increased serum osmolal gap (81 mOsm/kg) was also observed. The serum concentration of isopropanol decreased to 9.5 mmol/L 15.5 h after admission with an estimated half-life of elimination of 5-7 h. No serum acetone was detected throughout the patient's hospitalization. The identity of isopropanol was confirmed by gas chromatography/mass spectrometry. The patient remained awake and alert while in the hospital and was discharged 5 days after admission. These unusual findings raise some fundamental questions about the role of isopropanol conversion to acetone in the manifestation of symptoms usually associated with isopropanol intoxication.

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