Statistical Approach of Nutrient Optimization for Microalgae Cultivation

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

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Cobalt Chloride Hexahydrate Assisted in Reducing Energy Loss in Perovskite Solar Cells with Record Open-Circuit Voltage of 1.20 V

ACS Energy Letters ◽

10.1021/acsenergylett.1c00443 ◽

2021 ◽

pp. 2121-2128

Author(s):

Pengyang Wang ◽

Bingbing Chen ◽

Renjie Li ◽

Sanlong Wang ◽

Ningyu Ren ◽

...

Keyword(s):

Solar Cells ◽

Energy Loss ◽

Cobalt Chloride ◽

Open Circuit Voltage ◽

Perovskite Solar Cells ◽

Open Circuit ◽

Chloride Hexahydrate ◽

Cobalt Chloride Hexahydrate ◽

Reducing Energy

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Microalgae Cultivation in Different pH, Temperature and Media for Lipid Production

International Journal of Life Sciences ◽

10.3126/ijls.v8i2.10227 ◽

2014 ◽

Vol 8 (2) ◽

pp. 13-17 ◽

Cited By ~ 1

Author(s):

Dakshayini Jayaramareddy ◽

Ravikumar Krishnappa ◽

Girisha Sirangala Thimmappa

Keyword(s):

Lipid Production ◽

Life Sciences ◽

Nutrient Supply ◽

Polar Lipids ◽

Microalgae Cultivation ◽

Microalgal Biomass ◽

Chlorella Sp ◽

Nonpolar Lipids

Lipids produced by microalgal biomass can be grouped into nonpolar lipids and polar lipids, which can be easily converted into biofuels. Microalgal samples were collected from three different ponds of Bangalore and cultured in the laboratory to find the effect of different pH, temperature and media on the production of biomass and lipids. Among these, pH-9, temperature -25°C and Beneck’s media was most suitable for production of biomass (35.80 g/L) and lipids from the isolated microalgae Chlorella sp. compare to Chladospora sp. (13.33 g/L). Chlorella sp. Showed 0.32 (OD) at pH-9, 0.43 (OD) at temperature-25°C and 2.94 (OD) in Beneck’s media. Our result revealed that nutrient supply along with measured variables affects the production of biomass and lipids in different microalgae.DOI: http://dx.doi.org/10.3126/ijls.v8i2.10227 International Journal of Life Sciences Vol.8(2): 2014; 13-17

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COMPARATIVE STUDIES OF CELL GROWTH OF FRESHWATER MICROALGA CHLORELLA SP. IN PHOTOAUTOTROPHIC, HETEROTROPHIC AND MIXOTROPHIC CULTURES

Jurnal Teknologi ◽

10.11113/jt.v78.4349 ◽

2016 ◽

Vol 78 (7) ◽

Cited By ~ 1

Author(s):

Costantine Joannes ◽

Rachel Fran Mansa ◽

Suhaimi Md. Yasir ◽

Jedol Dayou

Keyword(s):

Cell Growth ◽

Cell Concentration ◽

Alternative Pathway ◽

Biodiesel Production ◽

Microalgae Cultivation ◽

Heterotrophic Culture ◽

Mixotrophic Culture ◽

Chlorella Sp ◽

Carbon Compounds ◽

Culture Mode

Lately, research on biodiesel production as a renewable and sustainable energy has become increasingly apparent due to the fact that fossil fuel is decreasing and the concern of global warming issues. The third generation of biofuel, which is microalgae-based biodiesel had gained interest over the last decade. The ability of microalgae to grow in various conditions is one of its advantages as the potential and promising feedstock for biodiesel. Microalgae can be cultivated in three modes such as photoautotrophic, heterotrophic and mixotrophic culture mode. Unlike photoautotrophic mode where light is required, the heterotrophic mode mainly utilized carbon compounds to grow. On the other hand, the mixotrophic mode is the condition where light and carbon compounds are supplied for microalgae culturing. This paper investigates the cell growth of Chlorella sp. cultivated in photoautotrophic, heterotrophic and mixotrophic culture mode. It was found that Chlorella sp. was capable of producing the highest cell concentration of 6.67 ± 0.56 x 106 cell mL-1 in the photoautotrophic mode for 23 days of cultivation period. This was 1.3 times and 3.2 times greater than the cell concentration in mixotrophic (5.02 ± 0.49 x 106 cell mL-1) and heterotrophic (2.03 ± 0.29 x 106 cell mL-1) culture, respectively. On the contrary, the highest specific growth rate obtained in the study was from heterotrophic mode (0.32 ± 0.04 day-1) followed by photoautotrophic and mixotrophic mode with 0.26 ± 0.05 day-1 and 0.20 ± 0.04 day-1, respectively. Chlorella sp. cell grew well under the photoautotrophic and mixotrophic mode. However, the insufficient of glucose level had contributed to lower cells productivity in the heterotrophic culture. Therefore, the mixotrophic mode could also be an alternative pathway in microalgae cultivation for biodiesel production if the glucose supplied was adequate and at the suitable level.

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