Utilization of de-oiled algal biomass for enhancing vehicular quality biodiesel production from Chlorella sp. in mixotrophic cultivation systems

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

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Mixotrophic cultivation of oleaginous Chlorella sp. KR-1 mediated by actual coal-fired flue gas for biodiesel production

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-014-1186-5 ◽

2014 ◽

Vol 37 (10) ◽

pp. 2083-2094 ◽

Cited By ~ 27

Author(s):

Ramasamy Praveenkumar ◽

Bohwa Kim ◽

Eunji Choi ◽

Kyubock Lee ◽

Sunja Cho ◽

...

Keyword(s):

Flue Gas ◽

Biodiesel Production ◽

Mixotrophic Cultivation ◽

Chlorella Sp

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Mixotrophic cultivation of Chlorella sp. BTA 9031 and Chlamydomonas sp. BTA 9032 isolated from coal field using various carbon sources for biodiesel production

Energy Conversion and Management ◽

10.1016/j.enconman.2016.07.033 ◽

2016 ◽

Vol 124 ◽

pp. 297-304 ◽

Cited By ~ 33

Author(s):

Madhumanti Mondal ◽

Ashmita Ghosh ◽

Aribam Subhalaxmi Sharma ◽

O.N. Tiwari ◽

K. Gayen ◽

...

Keyword(s):

Carbon Sources ◽

Biodiesel Production ◽

Mixotrophic Cultivation ◽

Coal Field ◽

Chlorella Sp ◽

Chlamydomonas Sp

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Algal-biomass Production from Chlorella sp. Using Hot and Cold Water Infusions of Poultry Droppings

Asian Journal of Biotechnology and Bioresource Technology ◽

10.9734/ajb2t/2018/v4i430048 ◽

2019 ◽

pp. 1-9

Author(s):

E. Effiong ◽

O. K. Agwa ◽

G. O. Abu

Keyword(s):

Algal Biomass ◽

Cold Water ◽

Algal Growth ◽

Dry Weight ◽

Hot Water ◽

Biodiesel Production ◽

Crude Fibre ◽

Physicochemical Composition ◽

Chlorella Sp ◽

Poultry Droppings

Background: Microbial biomass is a valuable resource to the development of sustainable energy. However, the challenge of having an effective media for energy production have adversely affected biotechnological development. This study was aimed at comparing algal biomass produced by Chlorella sp. using hot and cold water extracts of poultry droppings. Methodology: Fifteen grams (15 g) of poultry droppings was infused into 500 mL (cold and hot water) and allowed to stand for 48 h prior. Algal growth was monitored by cell dry weight and optical density readings taken at 620 nm using a spectrophotometer. Results: Physicochemical composition of the poultry droppings for cold water infusion revealed the following: pH, 6.7; conductivity, 3404.1 µs/cm; phosphate, 25.3 ppm; nitrate,1.88 ppm; phosphate, 25.3 ppm; Mg2+, 27.20 ppm; TOC, 38.03 ppm and COD, 53.8 ppm after 48 h. Whereas, the values obtained for hot extractions were: pH, 6.28; conductivity, 3.82 µs/cm; nitrate, 1.24 ppm; phosphate, 28.0 ppm; Mg2+, 19.85 ppm; TOC, 64.03 ppm and COD, 553.3 ppm. The proximate composition of extract revealed crude fibre, 17.69%, ash content, 24.16%; crude fibre, 22.7%; crude protein, 21.02%; crude fat, 3.19% and crude carbohydrate 23.02%. Microflora obtained from the biostability test revealed the presence of Proteus sp, Vibrio sp. and Salmonella sp. in cold extract while hot extract had Bacillus sp. and Serratia sp. The Chlorella sp. was identified using colonial and microscopic features. Biomass yield of 3.1 g/L and 2.8 g/l wet weight of Chlorella biomass was recorded for the cold and hot aqueous extracts of the poultry droppings respectively. Conclusion: This study revealed that hot poultry droppings extract (PDE) could offer a better feedstock for biodiesel production.

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Effects of Environmental Factors on Chlorella sp. microalgae for Biodiesel Production Purpose: Enhanced Lipid and Biomass Productivity

Recent Innovations in Chemical Engineering (Formerly Recent Patents on Chemical Engineering) ◽

10.2174/2405520410666171023144030 ◽

2018 ◽

Vol 10 (2) ◽

pp. 119-126

Author(s):

Saeed Dalirynezhad ◽

Ahmad Hallajisani ◽

Hamoon Nouri ◽

Abooali Golzary

Keyword(s):

Environmental Factors ◽

Biomass Productivity ◽

Biodiesel Production ◽

Chlorella Sp

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Biodiesel production from microalgae Dunaliella tertiolecta: a study on economic feasibility on large-scale cultivation systems

Biomass Conversion and Biorefinery ◽

10.1007/s13399-020-01191-1 ◽

2021 ◽

Author(s):

Akash Patel ◽

Bharat Gami ◽

Pankaj Patel ◽

Beena Patel

Keyword(s):

Large Scale ◽

Dunaliella Tertiolecta ◽

Economic Feasibility ◽

Biodiesel Production ◽

Cultivation Systems ◽

Large Scale Cultivation

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Comparison of Autotrophic and Mixotrophic Cultivation of Green Microalgal for Biodiesel Production

Energy Procedia ◽

10.1016/j.egypro.2014.07.088 ◽

2014 ◽

Vol 52 ◽

pp. 371-376 ◽

Cited By ~ 31

Author(s):

Yu-Ru Li ◽

Wen-Tien Tsai ◽

Yi-Chyun Hsu ◽

Meng-Zhi Xie ◽

Jen-Jeng Chen

Keyword(s):

Biodiesel Production ◽

Mixotrophic Cultivation

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COMPARATIVE STUDIES OF CELL GROWTH OF FRESHWATER MICROALGA CHLORELLA SP. IN PHOTOAUTOTROPHIC, HETEROTROPHIC AND MIXOTROPHIC CULTURES

Jurnal Teknologi ◽

10.11113/jt.v78.4349 ◽

2016 ◽

Vol 78 (7) ◽

Cited By ~ 1

Author(s):

Costantine Joannes ◽

Rachel Fran Mansa ◽

Suhaimi Md. Yasir ◽

Jedol Dayou

Keyword(s):

Cell Growth ◽

Cell Concentration ◽

Alternative Pathway ◽

Biodiesel Production ◽

Microalgae Cultivation ◽

Heterotrophic Culture ◽

Mixotrophic Culture ◽

Chlorella Sp ◽

Carbon Compounds ◽

Culture Mode

Lately, research on biodiesel production as a renewable and sustainable energy has become increasingly apparent due to the fact that fossil fuel is decreasing and the concern of global warming issues. The third generation of biofuel, which is microalgae-based biodiesel had gained interest over the last decade. The ability of microalgae to grow in various conditions is one of its advantages as the potential and promising feedstock for biodiesel. Microalgae can be cultivated in three modes such as photoautotrophic, heterotrophic and mixotrophic culture mode. Unlike photoautotrophic mode where light is required, the heterotrophic mode mainly utilized carbon compounds to grow. On the other hand, the mixotrophic mode is the condition where light and carbon compounds are supplied for microalgae culturing. This paper investigates the cell growth of Chlorella sp. cultivated in photoautotrophic, heterotrophic and mixotrophic culture mode. It was found that Chlorella sp. was capable of producing the highest cell concentration of 6.67 ± 0.56 x 106 cell mL-1 in the photoautotrophic mode for 23 days of cultivation period. This was 1.3 times and 3.2 times greater than the cell concentration in mixotrophic (5.02 ± 0.49 x 106 cell mL-1) and heterotrophic (2.03 ± 0.29 x 106 cell mL-1) culture, respectively. On the contrary, the highest specific growth rate obtained in the study was from heterotrophic mode (0.32 ± 0.04 day-1) followed by photoautotrophic and mixotrophic mode with 0.26 ± 0.05 day-1 and 0.20 ± 0.04 day-1, respectively. Chlorella sp. cell grew well under the photoautotrophic and mixotrophic mode. However, the insufficient of glucose level had contributed to lower cells productivity in the heterotrophic culture. Therefore, the mixotrophic mode could also be an alternative pathway in microalgae cultivation for biodiesel production if the glucose supplied was adequate and at the suitable level.

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