Mixotrophic cultivation of Chlorella sp. BTA 9031 and Chlamydomonas sp. BTA 9032 isolated from coal field using various carbon sources for biodiesel production

2016 ◽  
Vol 124 ◽  
pp. 297-304 ◽  
Author(s):  
Madhumanti Mondal ◽  
Ashmita Ghosh ◽  
Aribam Subhalaxmi Sharma ◽  
O.N. Tiwari ◽  
K. Gayen ◽  
...  
Keyword(s):  
Carbon Sources ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Coal Field ◽  
Chlorella Sp ◽  
Chlamydomonas Sp

Utilization of de-oiled algal biomass for enhancing vehicular quality biodiesel production from Chlorella sp. in mixotrophic cultivation systems

2018 ◽  
Vol 122 ◽  
pp. 80-88 ◽  
Author(s):  
Richa Katiyar ◽  
Randhir K. Bharti ◽  
B.R. Gurjar ◽  
Amit Kumar ◽  
Shalini Biswas ◽  
...  
Keyword(s):  
Algal Biomass ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Cultivation Systems ◽  
Chlorella Sp

scholarly journals Statistical Approach of Nutrient Optimization for Microalgae Cultivation

2020 ◽  
Vol 141 ◽  
pp. 03009
Author(s):  
Pichayatorn Bunkaew ◽  
Sasithorn Kongruang
Keyword(s):  
Light Intensity ◽  
Cobalt Chloride ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Microalgae Cultivation ◽  
Cultivation Time ◽  
Chlorella Sp ◽  
Chloride Hexahydrate ◽  
Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

Mixotrophic cultivation of oleaginous Chlorella sp. KR-1 mediated by actual coal-fired flue gas for biodiesel production

2014 ◽  
Vol 37 (10) ◽  
pp. 2083-2094 ◽  
Author(s):  
Ramasamy Praveenkumar ◽  
Bohwa Kim ◽  
Eunji Choi ◽  
Kyubock Lee ◽  
Sunja Cho ◽  
...  
Keyword(s):  
Flue Gas ◽  
Biodiesel Production ◽  
Mixotrophic Cultivation ◽  
Chlorella Sp

scholarly journals Comparison of Autotrophic and Mixotrophic Cultivation of Green Microalgal for Biodiesel Production

2014 ◽  
Vol 52 ◽  
pp. 371-376 ◽  
Author(s):  
Yu-Ru Li ◽  
Wen-Tien Tsai ◽  
Yi-Chyun Hsu ◽  
Meng-Zhi Xie ◽  
Jen-Jeng Chen
Keyword(s):  
Biodiesel Production ◽  
Mixotrophic Cultivation

scholarly journals COMPARATIVE STUDIES OF CELL GROWTH OF FRESHWATER MICROALGA CHLORELLA SP. IN PHOTOAUTOTROPHIC, HETEROTROPHIC AND MIXOTROPHIC CULTURES

2016 ◽  
Vol 78 (7) ◽  
Author(s):  
Costantine Joannes ◽  
Rachel Fran Mansa ◽  
Suhaimi Md. Yasir ◽  
Jedol Dayou
Keyword(s):  
Cell Growth ◽  
Cell Concentration ◽  
Alternative Pathway ◽  
Biodiesel Production ◽  
Microalgae Cultivation ◽  
Heterotrophic Culture ◽  
Mixotrophic Culture ◽  
Chlorella Sp ◽  
Carbon Compounds ◽  
Culture Mode

Lately, research on biodiesel production as a renewable and sustainable energy has become increasingly apparent due to the fact that fossil fuel is decreasing and the concern of global warming issues. The third generation of biofuel, which is microalgae-based biodiesel had gained interest over the last decade. The ability of microalgae to grow in various conditions is one of its advantages as the potential and promising feedstock for biodiesel. Microalgae can be cultivated in three modes such as photoautotrophic, heterotrophic and mixotrophic culture mode. Unlike photoautotrophic mode where light is required, the heterotrophic mode mainly utilized carbon compounds to grow. On the other hand, the mixotrophic mode is the condition where light and carbon compounds are supplied for microalgae culturing. This paper investigates the cell growth of Chlorella sp. cultivated in photoautotrophic, heterotrophic and mixotrophic culture mode. It was found that Chlorella sp. was capable of producing the highest cell concentration of 6.67 ± 0.56 x 106 cell mL-1 in the photoautotrophic mode for 23 days of cultivation period. This was 1.3 times and 3.2 times greater than the cell concentration in mixotrophic (5.02 ± 0.49 x 106 cell mL-1) and heterotrophic (2.03 ± 0.29 x 106 cell mL-1) culture, respectively. On the contrary, the highest specific growth rate obtained in the study was from heterotrophic mode (0.32 ± 0.04 day-1) followed by photoautotrophic and mixotrophic mode with 0.26 ± 0.05 day-1 and 0.20 ± 0.04 day-1, respectively. Chlorella sp. cell grew well under the photoautotrophic and mixotrophic mode. However, the insufficient of glucose level had contributed to lower cells productivity in the heterotrophic culture. Therefore, the mixotrophic mode could also be an alternative pathway in microalgae cultivation for biodiesel production if the glucose supplied was adequate and at the suitable level.  

scholarly journals Renewable Biodiesel Production from Oleaginous Yeast Biomass Using Industrial Wastes

2020 ◽  
Vol 141 ◽  
pp. 03010
Author(s):  
Sasithorn Kongruang ◽  
Sittiruk Roytrakul ◽  
Malinee Sriariyanun
Keyword(s):  
Fatty Acid ◽  
Second Generation ◽  
Carbon Sources ◽  
Morphological Characteristics ◽  
Rhodotorula Glutinis ◽  
Industrial Wastes ◽  
Biodiesel Production ◽  
Yeast Strains ◽  
Fatty Acid Profiling ◽  
Carbon Substrates

The accumulation lipid from oleaginous microorganisms is recognized as a second generation fuel. Biooil is known to as intracellular product of oily yeast utilizing various carbon substrates and converting different quantities of lipids in the form of triacylglycerols. This second generation fuel can be used to make biodiesel via a transesterification process. This study investigated the morphological characteristics of eight strains of Thai oleaginous yeasts via microscopy and analyzed the fatty acid profiling of yeasts cultured in three carbon sources: glucose, sugar cane molasses and crude glycerol in order to estimate biodiesel properties. To approach this goal, batch fermentations were used to culture eight yeast strains, Rhodosporidium toruloides TISTR 5123, TISTR 5154, TISTR 5149, Yarrowia lipolytica TISTR 5054, TISTR 5151, TISTR 5621, Rhodotorula glutinis TISTR 5159 and Rhodotorula graminis TISTR 5124 for 96 h under 30°C at 250 rpm. Result revealed that eight yeast strains contained significant amounts of fatty acids and lipids and accumulated mainly palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C 18:1) and linoleic acid (C18:2), and they are suitable for the production of biodiesel. Fatty acid productions and profiles indicated that these yeast strains can be potentially used as the triacylglycerols producers for biodiesel production.

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