Light intensity control as a strategy to improve lipid productivity in Chlorella sp. HS2 for biodiesel production

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

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Correction: Light Intensity Improves Growth, Lipid Productivity, and Fatty Acid Profile of Chlorococcum oleofaciens (Chlorophyceae) for Biodiesel Production

BioEnergy Research ◽

10.1007/s12155-020-10186-9 ◽

2020 ◽

Vol 13 (4) ◽

pp. 1246-1246

Author(s):

Marzie Rayati ◽

Houman Rajabi Islami ◽

Mehdi Shamsaie Mehrgan

Keyword(s):

Fatty Acid ◽

Light Intensity ◽

Fatty Acid Profile ◽

Lipid Productivity ◽

Biodiesel Production

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UV Mutagenesis as a Strategy to Enhance Growth and Lipid Productivity of Chlorella sp. 042

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.56862 ◽

2020 ◽

Vol 5 (3) ◽

pp. 218

Author(s):

Rike Rachmayati ◽

Eva Agustriana ◽

Delicia Yunita Rahman

Keyword(s):

Nile Red ◽

Lipid Productivity ◽

Biodiesel Production ◽

Wild Type ◽

Uv Mutagenesis ◽

Chlorella Sp ◽

Uv Mutation ◽

High Lipid ◽

Nile Red Fluorescence ◽

Alternative Feedstock

Microalgae appeared to be an alternative feedstock for renewable biodiesel production due to their capability to accumulate considerable amounts of lipids. In this study, mutagenesis using UVC light with different periods was applied to Chlorella sp. 042 to produce a microalgae strain with high lipid productivity of 45, 60, and 75 min. The Nile red fluorescence method was conducted to select a Chlorella sp. mutant with high neutral lipid and generated one mutant from every UV mutation period, M45-06, M60-02, and M75-21. All of the mutants have higher growth rates than the wild type. Chlorella sp. 042 M60-02 achieved the highest lipid productivity, with 34 mg L-1 day-1. Furthermore, as other major biochemical components, carbohydrate and protein contents were determined. Our results showed that all the mutants enhance their carbohydrate and protein contents compared to the wild type. However, mutations for more than 60 min do not intensely change the protein content of mutant microalgae. Gas chromatography-mass spectrophotometry analysis revealed that M60-02 mutant has similar FAME profiles with the wild type, which contain palmitic acid (C16:0), stearic acid (C 18:0), oleic acid (C18:1), and linoleic acid (C18:2). These results demonstrate that the UV mutation of Chlorella sp. 042 for 60 min is suitable as a source of biodiesel production.

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Characterization and identification of freshwater microalgal strains toward biofuel production

BioResources ◽

10.15376/biores.7.1.686-695 ◽

2011 ◽

Vol 7 (1) ◽

pp. 686-695

Author(s):

Xun Yang ◽

Pinghuai Liu ◽

Zongdi Hao ◽

Jie Shi ◽

Sen Zhang

Keyword(s):

Oleic Acid ◽

Lipid Content ◽

Biomass Concentration ◽

Lipid Productivity ◽

Biodiesel Production ◽

Biofuel Production ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Chlorella Sp ◽

High Lipid

Fifty-three algal cultures were isolated from freshwater lakes in Hainan, China. Four microalgal isolates were selected because they could be successfully cultivated at high density and demostrated a strong fluorescence after being stained with nile red. These cultures were identified as strains of Chlorella sp. C11, Chlamydomonas reinhardtii C22, Monoraphidium dybowskii C29, and Chlorella sp. HK12 through microscopic and 18S rDNA analysis. Under similar conditions, the lipid productivity of Chlorella sp. C11, Chla. reinhardtii C22, M. dybowskii C29 , and Chlorella sp. HK12 were 1.88, 2.79, 2.00, and 3.25 g L-1, respectively. Chla. reinhardtii C22 yielded a higher lipid content (51%), with a lower biomass concentration (5.47 g dwt L-1). Chlorella sp. HK12 reached a growth rate of 0.88 day-1 at OD540nm and yielded a biomass concentration of 7.56 g dwt L-1, with a high lipid content of 43%. Gas chromatography/ mass spectrometry analysis indicated that lipid fraction mainly comprises hydrocarbons including palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acids. Our results suggest that Chlorella sp. HK12 is a promising species for biodiesel production, because of its high lipid productivity and a relatively high content of oleic acid.

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Effect of Light Intensity, CO2 Gas Concentration, Culturing Period and Walne Nutrient Concentrations on Biomass and Lipid Productivity of Chlorella vulgaris in Sea Water Media

MATEC Web of Conferences ◽

10.1051/matecconf/201815603024 ◽

2018 ◽

Vol 156 ◽

pp. 03024

Author(s):

Timotius Candra Kusuma ◽

Anggun Rindang Pratiwi ◽

Septiandre ◽

Siti Zulaikah

Keyword(s):

Light Intensity ◽

Chlorella Vulgaris ◽

Nutrient Concentration ◽

Sea Water ◽

Lipid Productivity ◽

Biodiesel Production ◽

Nutrient Concentrations ◽

Gas Concentration ◽

Water Media ◽

Effect Of Light

The biomass and lipid productivity of Chlorella vulgaris cultured in sea water media were conducted in this study. The effect of light intensity (5000 and 10000 lux), CO2 gas concentration (0.03%, 1% and 2%), culturing period (7 and 17 days) and walne nutrient concentrations (0%, 0.05%, 0.1% and 0.3%) on biomass and lipid productivity of C. vulgaris cultured in photobioreactor were studied systematically. The biomass and lipid productivity were increased with increasing light intensity and CO2 gas concentration. Longer culturing period, C. vulgaris produced more biomass and lipid content. However, biomass and lipid productivity at shorter cultured period were higher than longer cultured period. The highest biomass productivity of 139 mg/L/d was obtained under the following condition: light intensity = 10000 lux, CO2 gas concentration = 2%, culturing period = 7 days, and walne nutrient concentration = 0.3%. The highest lipid productivity of 40.68 mg/L/d was obtained under the following condition: light intensity = 10000 lux, CO2 gas concentration = 2%, culturing period = 7 days, and walne nutrient concentration = 0.005%. This study shows that a microalga C. vulgaris was a potential candidate as a source of biodiesel production.

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