scholarly journals Detection of potential transgenic plant DNA recipients among soil bacteria

2007 ◽  
Vol 6 (1-2) ◽  
pp. 71-83 ◽  
Author(s):  
Jean-Michel Monier ◽  
Dominique Bernillon ◽  
Elizabeth Kay ◽  
Aurélie Faugier ◽  
Oleksandra Rybalka ◽  
...  
Keyword(s):  
Transgenic Plant ◽  
Soil Bacteria ◽  
Plant Dna

scholarly journals Antibiotic-resistant soil bacteria in transgenic plant fields

2008 ◽  
Vol 105 (10) ◽  
pp. 3957-3962 ◽  
Author(s):  
S. Demaneche ◽  
H. Sanguin ◽  
J. Pote ◽  
E. Navarro ◽  
D. Bernillon ◽  
...  
Keyword(s):  
Transgenic Plant ◽  
Soil Bacteria ◽  
Antibiotic Resistant

Assessing the survival of transgenic plant DNA in the human gastrointestinal tract

2004 ◽  
Vol 22 (2) ◽  
pp. 204-209 ◽  
Author(s):  
Trudy Netherwood ◽  
Susana M Martín-Orúe ◽  
Anthony G O'Donnell ◽  
Sally Gockling ◽  
Julia Graham ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Transgenic Plant ◽  
Human Gastrointestinal Tract ◽  
Plant Dna

scholarly journals Transformation of Acinetobacter sp. Strain BD413(pFG4ΔnptII) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

2000 ◽  
Vol 66 (3) ◽  
pp. 1237-1242 ◽  
Author(s):  
Kaare M. Nielsen ◽  
Jan D. van Elsas ◽  
Kornelia Smalla
Keyword(s):  
Transgenic Plant ◽  
Kanamycin Resistance ◽  
Soil Restoration ◽  
Dna Encoding ◽  
Sugar Beets ◽  
Soil Microcosms ◽  
Plant Dna ◽  
High Concentrations ◽  
Selection Of

ABSTRACT Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptIIgene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functionalnptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU ofAcinetobacter sp. cells from 109 CFU/g of soil to below detection. In contrast to a chromosomalnptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.

scholarly journals Transformation of Acinetobacter sp. Strain BD413 by Transgenic Sugar Beet DNA

1998 ◽  
Vol 64 (4) ◽  
pp. 1550-1554 ◽  
Author(s):  
Frank Gebhard ◽  
Kornelia Smalla
Keyword(s):  
Homologous Recombination ◽  
Sugar Beet ◽  
Transgenic Plant ◽  
Molecular Analysis ◽  
Plasmid Dna ◽  
Plant Dna ◽  
Laboratory Conditions ◽  
Acinetobacter Sp ◽  
Transgenic Sugar Beet

ABSTRACT The ability of Acinetobacter sp. strain BD413(pFG4ΔnptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.

scholarly journals Fate of transgenic plant DNA in the environment

2007 ◽  
Vol 6 (1-2) ◽  
pp. 15-35 ◽  
Author(s):  
Alessandra Pontiroli ◽  
Pascal Simonet ◽  
Asa Frostegard ◽  
Timothy M. Vogel ◽  
Jean-Michel Monier
Keyword(s):  
Transgenic Plant ◽  
Plant Dna

Quantitation of Transgenic Plant DNA in Leachate Water:  Real-Time Polymerase Chain Reaction Analysis

2005 ◽  
Vol 53 (15) ◽  
pp. 5858-5865 ◽  
Author(s):  
Robert H. Gulden ◽  
Sylvain Lerat ◽  
Miranda M. Hart ◽  
Jeff R. Powell ◽  
Jack T. Trevors ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Transgenic Plant ◽  
Real Time ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Plant Dna ◽  
Polymerase Chain ◽  
Leachate Water
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