Morphological and biochemical analyses of the salivary glands of the malaria vector, Anopheles darlingi

1999 ◽  
Vol 31 (3) ◽  
pp. 264-273 ◽  
Author(s):  
C.K. Moreira-Ferro ◽  
O. Marinotti ◽  
A.T. Bijovsky
1998 ◽  
Vol 7 (3) ◽  
pp. 257-264 ◽  
Author(s):  
C. K. Moreira‐Ferro ◽  
S. Daffre ◽  
A. A. James ◽  
O. Marinotti

2004 ◽  
Vol 13 (1) ◽  
pp. 73-88 ◽  
Author(s):  
E. Calvo ◽  
J. Andersen ◽  
I. M. Francischetti ◽  
M. deL. Capurro ◽  
A. G. deBianchi ◽  
...  

Author(s):  
Narissara Jariyapan ◽  
Wej Choochote ◽  
Atchariya Jitpakdi ◽  
Thasaneeya Harnnoi ◽  
Padet Siriyasatein ◽  
...  

Salivary gland proteins of the human malaria vector, Anopheles dirus B were determined and analyzed. The amount of salivary gland proteins in mosquitoes aged between 3 - 10 days was approximately 1.08 ± 0.04 µg/female and 0.1 ± 0.05 µg/male. The salivary glands of both sexes displayed the same morphological organization as that of other anopheline mosquitoes. In females, apyrase accumulated in the distal regions, whereas alpha-glucosidase was found in the proximal region of the lateral lobes. This differential distribution of the analyzed enzymes reflects specialization of different regions for sugar and blood feeding. SDS-PAGE analysis revealed that at least seven major proteins were found in the female salivary glands, of which each morphological region contained different major proteins. Similar electrophoretic protein profiles were detected comparing unfed and blood-fed mosquitoes, suggesting that there is no specific protein induced by blood. Two-dimensional polyacrylamide gel analysis showed the most abundant salivary gland protein, with a molecular mass of approximately 35 kilodaltons and an isoelectric point of approximately 4.0. These results provide basic information that would lead to further study on the role of salivary proteins of An. dirus B in disease transmission and hematophagy.


2019 ◽  
Vol 57 (3) ◽  
pp. 942-946 ◽  
Author(s):  
Pablo S Fontoura ◽  
Anderson S da Costa ◽  
Francismar S Ribeiro ◽  
Marcílio S Ferreira ◽  
Marcia C Castro ◽  
...  

Abstract Despite historical and contemporary evidence of its effectiveness, larval source management with insecticides remains little used by most malaria control programs worldwide. Here we show that environmentally safe biological larvicides under field conditions can significantly reduce anopheline larval density in fish farming ponds that have became major larval habitats across the Amazon Basin. Importantly, the primary local malaria vector, Anopheles darlingi Root (Diptera: Culicidae), feeds and rests predominantly outdoors, being little affected by interventions such as long-lasting insecticidal bed net distribution and indoor residual spraying. We found >95% reduction in late-instar density up to 7 d after the first application of VectoMax FG or VectoLex CG (both from Valent BioSciences), and up to 21 d after larvicide reapplication in fish ponds (n = 20) situated in the main residual malaria pocket of Brazil, irrespective of the formulation or dosage (10 or 20 kg/ha) used. These results are consistent with a substantial residual effect upon retreatment and support the use of biological larvicides to reduce the density of anopheline larvae in this and similar settings across the Amazon where larval habitats are readily identified and accessible.


BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 57 ◽  
Author(s):  
Eric Calvo ◽  
Van M Pham ◽  
Osvaldo Marinotti ◽  
John F Andersen ◽  
Jose MC Ribeiro

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