scholarly journals The transcriptome of adult female Anopheles darlingi salivary glands

2004 ◽  
Vol 13 (1) ◽  
pp. 73-88 ◽  
Author(s):  
E. Calvo ◽  
J. Andersen ◽  
I. M. Francischetti ◽  
M. deL. Capurro ◽  
A. G. deBianchi ◽  
...  
1998 ◽  
Vol 7 (3) ◽  
pp. 257-264 ◽  
Author(s):  
C. K. Moreira‐Ferro ◽  
S. Daffre ◽  
A. A. James ◽  
O. Marinotti

1993 ◽  
Vol 179 (1) ◽  
pp. 273-287
Author(s):  
J. M. Ribeiro ◽  
R. H. Nussenzveig

Salivary gland homogenates from adult female Anopheles albimanus mosquitoes relaxed aortic rings preconstricted with noradrenaline (NA). This relaxation is slow and is due to destruction of NA. Incubation of NA with the homogenate yielded a product with a spectrum consistent with the corresponding adrenochrome. Oxidation of NA was enhanced by a superoxide generation system and inhibited by the combined action of superoxide dismutase and catalase. Additionally, peroxidase activity on both synthetic (o-dianisidine) and biologically active (serotonin) substrates was also present in the salivary gland homogenates, this latter activity requiring hydrogen peroxide. Noradrenaline oxidation, serotonin and o-dianisidine peroxidation and vasodilation all co-elute with a heme protein of relative molecular mass 50,000, as determined by molecular sieving chromatography. Peroxidase activity was localized in the posterior (female-specific) lobes of salivary glands and was also detected in nitrocellulose membranes probed by hungry mosquitoes. Protein and peroxidase activities were significantly lower in salivary glands of mosquitoes after probing and feeding on blood. It is suggested that adult female Anopheles albimanus mosquitoes contain a salivary heme peroxidase that functions during blood finding and blood feeding by destroying hemostatically active biogenic amines released by the vertebrate host during tissue destruction.


1995 ◽  
Vol 81 (3) ◽  
pp. 239-248 ◽  
Author(s):  
C.T. Smartt ◽  
A.P. Kim ◽  
G.L. Grossman ◽  
A.A. James

1999 ◽  
Vol 31 (3) ◽  
pp. 264-273 ◽  
Author(s):  
C.K. Moreira-Ferro ◽  
O. Marinotti ◽  
A.T. Bijovsky

1995 ◽  
Vol 198 (1) ◽  
pp. 167-174 ◽  
Author(s):  
M G Novak ◽  
J M Ribeiro ◽  
J G Hildebrand

A dense plexus of axons, immunoreactive to antisera against 5-hydroxytryptamine (5-HT, serotonin) and surrounding the proximal medial lobe of the salivary gland of adult female Aedes aegypti mosquitoes, was demonstrated by means of whole-mount fluorescence immunocytochemistry. This innervation originates in the stomatogastric nervous system. 5-HT-immunoreactive innervation is absent in male salivary glands, suggesting that 5-HT is involved in blood-feeding. Furthermore, female mosquitoes treated with the 5-HT-depleting agent alpha-methyltryptophan (AMTP) and then allowed to feed on a rat exhibited a significantly longer mean probing period and a lower blood-feeding success rate than did control mosquitoes. When female mosquitoes were experimentally induced to salivate into mineral oil, AMTP-treated individuals secreted significantly less saliva than did control mosquitoes. These samples of saliva also contained significantly lower concentrations of apyrase, an enzyme important in blood-feeding. Injection of 5-HT into both AMTP-treated and control mosquitoes elicited significant increases in the volume of secreted saliva and/or its apyrase content. We conclude that 5-HT plays an important role in the control of salivation in adult female A. aegypti.


1969 ◽  
Vol 47 (6) ◽  
pp. 1341-1344 ◽  
Author(s):  
J. Gosbee ◽  
J. R. Allen ◽  
A. S. West

Salivary glands of adult female blackflies of known ages were examined by histological methods. These studies showed that each of the paired salivary glands consists of two parts: a reservoir composed of relatively small cells, and a U-shaped lobe composed of larger secretory cells. The secretory cells progressively accumulate a PAS-positive secretory substance during the first 48 h of adult life. The PAS-positive material is lost from the secretory cells when the blackfly takes a blood meal. During the first 24 h after a blood meal the secretory cells again synthesize some PAS-positive material, but in some individuals the resynthesis is still not complete after 72 h. Limited examination of the salivary glands of males was carried out.


1997 ◽  
Vol 84 (1) ◽  
pp. 69-74 ◽  
Author(s):  
A. M. Guimarães ◽  
J. D. Lima ◽  
M. F. B. Ribeiro ◽  
E. R. S. Camargos ◽  
I. A. Bozzi

Author(s):  
P.J. Dailey

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.


Sign in / Sign up

Export Citation Format

Share Document