This research aim to validation of a simple, rapid and accurate HPLC-UV method for the quantification of andrographolide isolated from Andrographis paniculata Ness in Self Nano Emulsifying Drug Delivery System (SNEDDS) formulation
during the dissolution test. The assay was performed using a XTerra® MS C18 column (150 mm X 4.6 mm, five μm) with
a mobile phase of methanol and water (70: 30), at 0.8 mL/min flow rate and UV detection of 229 nm. Simulation gastric
fluid (SGF) and intestinal fluid (SIF) were prepared as dissolution medium. The validation parameter was conducted
including the test on linearity, precision, accuracy, LOD, and LOQ. The result showed an excellent linearity with r = 0.999
and good selectivity for both medium dissolution. The method showed sufficient precision, with a relative standard
deviation (RSD) smaller than % Horwitz. The accuracy reported as % recovery was found to be 102.61 and 101.17 % in
each SGF and SIF dissolution medium. LOD and LOQ were found 0.46 and 1.40 in SGF medium, 0.87 and 2.64 in SIF
medium. In conclusion, the HPLC method developed showed specificity and selectivity with linearity in the working range,
good precision and accuracy and suitable for quantification andrographolide in SNEDDS formulation.
