Somatostatin receptor scintigraphy in patients with cat-scratch disease

2006 ◽  
Vol 45 (04) ◽  
pp. 160-162
Author(s):  
C. Piswanger-Soelkner ◽  
R. W. Lipp ◽  
F. Daxböck ◽  
W. J. Schnedl ◽  
S. Hoier ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Somatostatin Receptor ◽  
Somatostatin Receptor Scintigraphy ◽  
Cat Scratch Disease ◽  
Igg Antibodies ◽  
Antibody Testing ◽  
Specific Pcr ◽  
Receptor Scintigraphy ◽  
Antibody Tests ◽  
Immunofluorescence Antibody

SummaryAim: Somatostatin receptor scintigraphy images various neoplastic, granulomatous, and auto-immun diseases. Cat-scratch disease in an infectious granulomatous disease usually affecting the lymphnodes. It is not known whether cat-scratch disease provides positive somatostatin receptor scintigrams. Patients, methods: Twelve patients with lymphadenitis and suspected cat-scratch disease were investigated by immunofluorescence antibody testing and somatostatin receptor scintigraphy. Suppurated lymphnodes were extracted or drained and Bartonella henselae specific PCR was then performed. Results: Eleven of 12 patients showed IgG antibodies against B. henselea. SRS showed positive scintigraphic results in 6 of 11 patients with CSD. B. henselae DNA was detected in tissue of lymphnodes from 4 of 5 patients with lymphnode extraction or lymphnode drainage. SRS demonstrated positive scintigrams in all patients with a positive PCR. In one patient with suspected CSD SRS was negative as well as antibody testing. Conclusion: Somatostatin receptor scintigraphy correlated with positive Bartonella henselae specific PCR tests and positive Bartonella henselae specific antibody tests in patients with CSD.

scholarly journals Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M against Bartonella henselae and Bartonella quintana

2002 ◽  
Vol 9 (5) ◽  
pp. 1004-1009 ◽  
Author(s):  
M. Maurin ◽  
J. M. Rolain ◽  
D. Raoult
Keyword(s):  
Bartonella Henselae ◽  
Fluorescent Antibody ◽  
The Other ◽  
Cat Scratch Disease ◽  
Indirect Fluorescent Antibody ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Bartonella Quintana ◽  
Antibody Tests ◽  
Higher Sensitivity

ABSTRACT We compared the sensitivities and specificities of indirect fluorescent antibody tests developed in our laboratory and commercially available from Focus Technologies (FT; formerly MRL Diagnostic) for detection of serum antibodies to Bartonella spp. Serum samples tested were from patients with culture- or PCR-confirmed Bartonella quintana or B. henselae infections causing cat scratch disease (CSD), chronic bacteremia, or endocarditis. At a cutoff titer of 64, the FT test had higher sensitivity than our in-house test in detecting anti-B. henselae immunoglobulin G (IgG) antibodies in CSD patients (91.2 versus 52.9%; P < 0.001). The specificity in serum samples from 85 control patients was, however, lower with the FT test (87%) than with the in-house test (98.8%) (P = 0.002). A cutoff titer of 128 improves the specificity for the FT test but lowers the sensitivity to 85%. For patients infected with B. henselae, our in-house test, but not the FT test, enabled endocarditis to be detected more reliably. With the in-house test, titers of IgG against B. henselae of ≥1,024 were found only in endocarditis patients and not in CSD patients. With the FT test, 19.1% of CSD patients had titers of IgG against B. henselae of ≥1,024 (P < 0.001). Our in-house technique also improved detection of anti-B. quintana antibodies in homeless patients with endocarditis. IgG titers of ≥1,024 were present in 75% of serum samples, but only in 16.7% of serum samples with the FT test (P = 0.004). Since each test has advantages over the other, the serological diagnosis of Bartonella infections would benefit if both tests were used concurrently.

scholarly journals Somatostatin receptor scintigraphy in the initial staging of Hodgkin's disease

1996 ◽  
Vol 93 (1) ◽  
pp. 96-103 ◽  
Author(s):  
P. J. van den Anker-Lugtenburg ◽  
E. P. Krenning ◽  
H. Y. Oei ◽  
M. P. Van Hagen ◽  
C. J. H. Gerrits ◽  
...  
Keyword(s):  
Hodgkin's Disease ◽  
Hodgkin’S Disease ◽  
Somatostatin Receptor ◽  
Somatostatin Receptor Scintigraphy ◽  
Receptor Scintigraphy ◽  
Initial Staging

The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease

2004 ◽  
Vol 53 (12) ◽  
pp. 1221-1227 ◽  
Author(s):  
Christine M Litwin ◽  
Joel M Johnson ◽  
Thomas B Martins
Keyword(s):  
Coxiella Burnetii ◽  
Brucella Melitensis ◽  
Bartonella Henselae ◽  
Immunoblot Analysis ◽  
Cross Reactivity ◽  
Cosmid Library ◽  
Cat Scratch Disease ◽  
Igg Antibodies ◽  
Bacillary Angiomatosis ◽  
Dihydrolipoamide Succinyltransferase

Bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. A recombinant clone expressing an immunoreactive antigen of B. henselae was isolated by screening a genomic DNA cosmid library by Western blotting with sera pooled from patients positive for B. henselae IgG antibodies by indirect immunofluorescence (IFA). The deduced amino acid sequence of the 43.7 kDa encoded protein was found to be 76.3 % identical to the dihydrolipoamide succinyltransferase enzyme (SucB) of Brucella melitensis. SucB has been shown to be an immunogenic protein during infections by Brucella melitensis, Coxiella burnetii and Bartonella vinsonii. The agreement between reactivity with a recombinant SucB fusion protein on immunoblot analysis and the results obtained by IFA was 55 % for IFA-positive sera and 88 % for IFA-negative sera. Cross-reactivity was observed with sera from patients with antibodies against Brucella melitensis, Mycoplasma pneumoniae, Francisella tularensis, Coxiella burnetii and Rickettsia typhi.

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