scholarly journals Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M against Bartonella henselae and Bartonella quintana

2002 ◽  
Vol 9 (5) ◽  
pp. 1004-1009 ◽  
Author(s):  
M. Maurin ◽  
J. M. Rolain ◽  
D. Raoult
Keyword(s):  
Bartonella Henselae ◽  
Fluorescent Antibody ◽  
The Other ◽  
Cat Scratch Disease ◽  
Indirect Fluorescent Antibody ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Bartonella Quintana ◽  
Antibody Tests ◽  
Higher Sensitivity

ABSTRACT We compared the sensitivities and specificities of indirect fluorescent antibody tests developed in our laboratory and commercially available from Focus Technologies (FT; formerly MRL Diagnostic) for detection of serum antibodies to Bartonella spp. Serum samples tested were from patients with culture- or PCR-confirmed Bartonella quintana or B. henselae infections causing cat scratch disease (CSD), chronic bacteremia, or endocarditis. At a cutoff titer of 64, the FT test had higher sensitivity than our in-house test in detecting anti-B. henselae immunoglobulin G (IgG) antibodies in CSD patients (91.2 versus 52.9%; P < 0.001). The specificity in serum samples from 85 control patients was, however, lower with the FT test (87%) than with the in-house test (98.8%) (P = 0.002). A cutoff titer of 128 improves the specificity for the FT test but lowers the sensitivity to 85%. For patients infected with B. henselae, our in-house test, but not the FT test, enabled endocarditis to be detected more reliably. With the in-house test, titers of IgG against B. henselae of ≥1,024 were found only in endocarditis patients and not in CSD patients. With the FT test, 19.1% of CSD patients had titers of IgG against B. henselae of ≥1,024 (P < 0.001). Our in-house technique also improved detection of anti-B. quintana antibodies in homeless patients with endocarditis. IgG titers of ≥1,024 were present in 75% of serum samples, but only in 16.7% of serum samples with the FT test (P = 0.004). Since each test has advantages over the other, the serological diagnosis of Bartonella infections would benefit if both tests were used concurrently.

A CASE OF CAT SCRATCH DISEASE DIAGNOSED BY INDIRECT FLUORESCENT ANTIBODY ASSAY OF IGM SPECIFIC FOR A JAPANESE STRAIN OF Bartonella henselae

2019 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Sho-Hei Uchi ◽  
Ryoji Yanai ◽  
Hidehiro Tsuneoka ◽  
Ken-ichiro Otsuyama ◽  
Koh-Hei Sonoda ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Fluorescent Antibody ◽  
Cat Scratch Disease ◽  
Indirect Fluorescent Antibody ◽  
Antibody Assay ◽  
Japanese Strain

Somatostatin receptor scintigraphy in patients with cat-scratch disease

2006 ◽  
Vol 45 (04) ◽  
pp. 160-162
Author(s):  
C. Piswanger-Soelkner ◽  
R. W. Lipp ◽  
F. Daxböck ◽  
W. J. Schnedl ◽  
S. Hoier ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Somatostatin Receptor ◽  
Somatostatin Receptor Scintigraphy ◽  
Cat Scratch Disease ◽  
Igg Antibodies ◽  
Antibody Testing ◽  
Specific Pcr ◽  
Receptor Scintigraphy ◽  
Antibody Tests ◽  
Immunofluorescence Antibody

SummaryAim: Somatostatin receptor scintigraphy images various neoplastic, granulomatous, and auto-immun diseases. Cat-scratch disease in an infectious granulomatous disease usually affecting the lymphnodes. It is not known whether cat-scratch disease provides positive somatostatin receptor scintigrams. Patients, methods: Twelve patients with lymphadenitis and suspected cat-scratch disease were investigated by immunofluorescence antibody testing and somatostatin receptor scintigraphy. Suppurated lymphnodes were extracted or drained and Bartonella henselae specific PCR was then performed. Results: Eleven of 12 patients showed IgG antibodies against B. henselea. SRS showed positive scintigraphic results in 6 of 11 patients with CSD. B. henselae DNA was detected in tissue of lymphnodes from 4 of 5 patients with lymphnode extraction or lymphnode drainage. SRS demonstrated positive scintigrams in all patients with a positive PCR. In one patient with suspected CSD SRS was negative as well as antibody testing. Conclusion: Somatostatin receptor scintigraphy correlated with positive Bartonella henselae specific PCR tests and positive Bartonella henselae specific antibody tests in patients with CSD.

The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease

2004 ◽  
Vol 53 (12) ◽  
pp. 1221-1227 ◽  
Author(s):  
Christine M Litwin ◽  
Joel M Johnson ◽  
Thomas B Martins
Keyword(s):  
Coxiella Burnetii ◽  
Brucella Melitensis ◽  
Bartonella Henselae ◽  
Immunoblot Analysis ◽  
Cross Reactivity ◽  
Cosmid Library ◽  
Cat Scratch Disease ◽  
Igg Antibodies ◽  
Bacillary Angiomatosis ◽  
Dihydrolipoamide Succinyltransferase

Bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. A recombinant clone expressing an immunoreactive antigen of B. henselae was isolated by screening a genomic DNA cosmid library by Western blotting with sera pooled from patients positive for B. henselae IgG antibodies by indirect immunofluorescence (IFA). The deduced amino acid sequence of the 43.7 kDa encoded protein was found to be 76.3 % identical to the dihydrolipoamide succinyltransferase enzyme (SucB) of Brucella melitensis. SucB has been shown to be an immunogenic protein during infections by Brucella melitensis, Coxiella burnetii and Bartonella vinsonii. The agreement between reactivity with a recombinant SucB fusion protein on immunoblot analysis and the results obtained by IFA was 55 % for IFA-positive sera and 88 % for IFA-negative sera. Cross-reactivity was observed with sera from patients with antibodies against Brucella melitensis, Mycoplasma pneumoniae, Francisella tularensis, Coxiella burnetii and Rickettsia typhi.

scholarly journals Seroprevalence of Toxoplasma gondii infection in swine matrices in Nova Mutum and Diamantino, Mato Grosso, Brazil

2010 ◽  
Vol 19 (4) ◽  
pp. 254-255 ◽  
Author(s):  
Lívia Saab Muraro ◽  
João Garcia Caramori Júnior ◽  
Maria Regina Reis Amendoeira ◽  
Joyce Alves Pereira ◽  
João Xavier de Oliveira Filho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
The State ◽  
Indirect Fluorescent Antibody ◽  
Risk Of Infection ◽  
Serum Samples ◽  
Mato Grosso ◽  
Toxoplasma Gondii Infection

This report aimed to assess the seroprevalence of Toxoplasma gondii infection in 708 swine matrices in Nova Mutum and Diamantino in the state of Mato Grosso, Central-West Brazil. Serum samples were examined by indirect fluorescent antibody test (IFAT). It was found a seroprevalence of 12.8%, considering titers >64. Therefore, the data reinforce the need for appropriate management of swine raising to minimize the risk of infection of pigs with T. gondii.

scholarly journals Cat-Scratch disease in Crete: an update

2011 ◽  
Vol 3 (2) ◽  
pp. 15 ◽  
Author(s):  
Georgios Minadakis ◽  
Emmanouil Angelakis ◽  
Dimosthenis Chochlakis ◽  
Yannis Tselentis ◽  
Anna Psaroulaki
Keyword(s):  
Bartonella Henselae ◽  
Immunofluorescence Assay ◽  
Positive Lymph Node ◽  
Cat Scratch Disease ◽  
Outdoor Activity ◽  
Pcr Assay ◽  
Serum Samples ◽  
Molecular Assays ◽  
Number Of Patients ◽  
Whole Blood Sample

There are few epidemiological and clinical studies about the presence of cat scratch disease (CSD) on the island of Crete. The objective of this study was to analyze a large number of patients with suspected CSD to define the frequency of <em>Bartonella</em> infections in Crete. From January 2005 to October 2008, we studied patients with suspected CSD from hospitals in Crete. Sera of the referred patients were tested by immunofluorescence assay (IFA). For some patients, we also received lymph nodes and blood samples that we tested for the presence of <em>Bartonella henselae</em> by molecular assays. Overall, we tested 507 serum samples and we found 56 (11%) cases of CSD. PCR assay was positive for 2 patients; one had a <em>B. henselae</em> positive lymph node and the other a positive whole blood sample. Significantly more CSD cases (62.5%, 35 of 56) were reported in children than in infants and adults (P&lt;0.05). Moreover, we identified that most cases of CSD occurred between May and September (P=0.002) and December and January. CSD is prevalent in Crete and is mostly associated with an increase in outdoor activity.

scholarly journals Variations in the detection of anti-PEDV antibodies in serum samples using three diagnostic tests – short communication

2018 ◽  
Vol 66 (2) ◽  
pp. 337-342 ◽  
Author(s):  
Jan Plut ◽  
Ivan Toplak ◽  
Marina Štukelj
Keyword(s):  
Clinical Signs ◽  
Elisa Test ◽  
Diagnostic Methods ◽  
P Value ◽  
Serum Samples ◽  
Blocking Elisa ◽  
Test Kit ◽  
Two Samples ◽  
Antibody Tests ◽  
Higher Sensitivity

Over the last few years several porcine epidemic diarrhoea (PED) outbreaks have been discovered in Europe including the first PED case in Slovenia in January 2015. The aim of this study was to determine when PED virus (PEDV) infection started in Slovenia. Serum samples collected between 2012 and 2016 were tested. Three hundred and seventy-five serum samples were collected from 132 Slovenian small, one-site pig farms. Samples were tested for PEDV antibodies utilising three different serological methods: commercially-available indirect ELISA, in-house blocking ELISA test and Immunoperoxidase Monolayer Assay (IPMA) test. One hundred and seventy (45.33%) tested samples were found positive by the commercially-available ELISA test kit, and 10 (5.68%) of these 170 samples found positive were positive by the in-house blocking ELISA. Only these 10 samples were collected from a farm where clinical signs of PED infection had been observed and PEDV was confirmed by RT-PCR methodology; the other 160 samples were collected randomly. Thirty-two samples with the highest S/P value obtained with the commercial ELISA were all negative with IPMA. Reasons for the high variance in the results obtained remain unclear; more research is required to ensure higher sensitivity and specificity in terms of PEDV antibody tests and other PED diagnostic methods.

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