The Bartonella henselae sucB gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease

2004 ◽  
Vol 53 (12) ◽  
pp. 1221-1227 ◽  
Author(s):  
Christine M Litwin ◽  
Joel M Johnson ◽  
Thomas B Martins
Keyword(s):  
Coxiella Burnetii ◽  
Brucella Melitensis ◽  
Bartonella Henselae ◽  
Immunoblot Analysis ◽  
Cross Reactivity ◽  
Cosmid Library ◽  
Cat Scratch Disease ◽  
Igg Antibodies ◽  
Bacillary Angiomatosis ◽  
Dihydrolipoamide Succinyltransferase

Bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. A recombinant clone expressing an immunoreactive antigen of B. henselae was isolated by screening a genomic DNA cosmid library by Western blotting with sera pooled from patients positive for B. henselae IgG antibodies by indirect immunofluorescence (IFA). The deduced amino acid sequence of the 43.7 kDa encoded protein was found to be 76.3 % identical to the dihydrolipoamide succinyltransferase enzyme (SucB) of Brucella melitensis. SucB has been shown to be an immunogenic protein during infections by Brucella melitensis, Coxiella burnetii and Bartonella vinsonii. The agreement between reactivity with a recombinant SucB fusion protein on immunoblot analysis and the results obtained by IFA was 55 % for IFA-positive sera and 88 % for IFA-negative sera. Cross-reactivity was observed with sera from patients with antibodies against Brucella melitensis, Mycoplasma pneumoniae, Francisella tularensis, Coxiella burnetii and Rickettsia typhi.

scholarly journals Development of an Immunoglobulin M Capture-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Acute Infections with Bartonella henselae

2008 ◽  
Vol 16 (2) ◽  
pp. 282-284 ◽  
Author(s):  
John G. Hoey ◽  
Fernando Valois-Cruz ◽  
Hannah Goldenberg ◽  
Yekaterina Voskoboynik ◽  
Jenna Pfiffner ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Bartonella Henselae ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cat Scratch Disease ◽  
Specific Enzyme ◽  
Negative Patient ◽  
Bacillary Angiomatosis ◽  
Acute Infections

ABSTRACT We describe the development of an immunoglobulin M-specific enzyme-linked immunosorbent assay for the detection of an early antibody response to Bartonella henselae, the causative agent of cat scratch disease, bacillary angiomatosis, and endocarditis. This assay discriminates between B. henselae-positive and -negative patient samples with sensitivity and specificity values of 100% and 97.1%, respectively.

scholarly journals Seroprevalence of Antibodies to Bartonella henselae in Patients with Cat Scratch Disease and in Healthy Controls: Evaluation and Comparison of Two Commercial Serological Tests

1998 ◽  
Vol 5 (4) ◽  
pp. 486-490 ◽  
Author(s):  
Anna Sander ◽  
Miriam Posselt ◽  
Karin Oberle ◽  
Wolfgang Bredt
Keyword(s):  
Sensitivity And Specificity ◽  
Close Contact ◽  
Bartonella Henselae ◽  
Vero Cells ◽  
Cross Reactivity ◽  
Sufficient Evidence ◽  
Healthy Controls ◽  
Cat Scratch Disease ◽  
Serological Tests ◽  
Bartonella Quintana

ABSTRACT Serologic testing for the presence of antibodies toBartonella henselae is a widely accepted diagnostic procedure for laboratory confirmation of the diagnosis of cat scratch disease (CSD). In this study a commercially available indirect immunofluorescence assay (IFA) based on B. henselae-infected human larynx carcinoma cells (test A) was evaluated. Sera from 42 patients with CSD (20 confirmed by PCR) and 270 sera from healthy controls (consisting of 63 cat owners, 65 individuals whose last close contact with cats was >6 months previously, and 142 persons who had never been exposed to cats) were investigated for antibodies to B. henselae. All patients with CSD had titers of immunoglobulin G (IgG) to B. henselae of 128 or higher (test A; sensitivity, 100%). Of the 270 controls 189 (70%) were seronegative (titer, <64), 38 (14.1%) had titers of 64, 30 (11.1%) had titers of 128, 9 (3.3%) had titers of 256, and 4 (1.5%) had high titers, 512 (test A; specificity, 70%). Of the cat owners and individuals who had never had close contact with cats, 71.4 and 71.12%, respectively, were seronegative, and titers of 64, 128, 256, and 512 were found in 14.3 and 16.2%, 1.6 and 10.5%, 9.5 and 0.7%, and 3.2 and 1.4%, respectively. The sera from the patients and from the first 100 healthy adults without a history of close contact with cats were additionally tested with a second commercially available IFA, based on Vero cells infected withB. henselae and Bartonella quintana (test B). The sensitivity and specificity of test B were 93 and 73%, respectively. For patients with CSD the cross-reactivity betweenB. henselae and B. quintana in this test was 95%. Both systems are highly sensitive but less specific for detection of IgG antibodies to B. henselae in samples from patients with clinically apparent CSD. For detection of IgM antibodies, test A seems to be more sensitive (88%) and more specific (95%) than test B (sensitivity and specificity of 64 and 86%, respectively). The data show that the seroprevalence of antibodies toB. henselae in German individuals is high (30%). Low antibody levels are not sufficient evidence of active or prior infection.

scholarly journals Murine Model of Bartonella henselae Infection in the Immunocompetent Host

1998 ◽  
Vol 66 (11) ◽  
pp. 5534-5536 ◽  
Author(s):  
T. Regnath ◽  
M. E. A. Mielke ◽  
M. Arvand ◽  
H. Hahn
Keyword(s):  
Immune Response ◽  
Liver Tissue ◽  
Bartonella Henselae ◽  
Granulomatous Inflammation ◽  
Peliosis Hepatis ◽  
Cat Scratch Disease ◽  
Immunocompetent Host ◽  
Bacillary Angiomatosis ◽  
Model Following ◽  
Intraperitoneal Infection

ABSTRACT Bartonella henselae is an emerging pathogen causing cat scratch disease, bacillary angiomatosis, and peliosis hepatis. Progress in understanding the pathogenesis of and the immune response to these infections has been limited by the lack of an animal model. Following intraperitoneal infection of C57BL/6 mice withB. henselae, organs were cleared of cultivatable bacteria within 6 days. In contrast, B. henselae DNA could be detected in liver tissue for at least 3 months. Liver tissue showed granulomatous inflammation reaching its highest degree of intensity during the fourth week of infection and resolving within 12 weeks postinfection. This mouse model is applicable to the study of the pathogenesis of B. henselae and the immune response to this pathogen in the immunocompetent host.

Somatostatin receptor scintigraphy in patients with cat-scratch disease

2006 ◽  
Vol 45 (04) ◽  
pp. 160-162
Author(s):  
C. Piswanger-Soelkner ◽  
R. W. Lipp ◽  
F. Daxböck ◽  
W. J. Schnedl ◽  
S. Hoier ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Somatostatin Receptor ◽  
Somatostatin Receptor Scintigraphy ◽  
Cat Scratch Disease ◽  
Igg Antibodies ◽  
Antibody Testing ◽  
Specific Pcr ◽  
Receptor Scintigraphy ◽  
Antibody Tests ◽  
Immunofluorescence Antibody

SummaryAim: Somatostatin receptor scintigraphy images various neoplastic, granulomatous, and auto-immun diseases. Cat-scratch disease in an infectious granulomatous disease usually affecting the lymphnodes. It is not known whether cat-scratch disease provides positive somatostatin receptor scintigrams. Patients, methods: Twelve patients with lymphadenitis and suspected cat-scratch disease were investigated by immunofluorescence antibody testing and somatostatin receptor scintigraphy. Suppurated lymphnodes were extracted or drained and Bartonella henselae specific PCR was then performed. Results: Eleven of 12 patients showed IgG antibodies against B. henselea. SRS showed positive scintigraphic results in 6 of 11 patients with CSD. B. henselae DNA was detected in tissue of lymphnodes from 4 of 5 patients with lymphnode extraction or lymphnode drainage. SRS demonstrated positive scintigrams in all patients with a positive PCR. In one patient with suspected CSD SRS was negative as well as antibody testing. Conclusion: Somatostatin receptor scintigraphy correlated with positive Bartonella henselae specific PCR tests and positive Bartonella henselae specific antibody tests in patients with CSD.

scholarly journals Conservation of the 17-Kilodalton Antigen Gene within the Genus Bartonella

2000 ◽  
Vol 7 (2) ◽  
pp. 251-257 ◽  
Author(s):  
Debra Sweger ◽  
Sandra Resto-Ruiz ◽  
David P. Johnson ◽  
Michael Schmiederer ◽  
Noel Hawke ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Sequence Divergence ◽  
Pcr Amplification ◽  
Immunoblot Analysis ◽  
Cross Reactivity ◽  
Rabbit Serum ◽  
Human Pathogens ◽  
Chromosomal Dna ◽  
Serum Samples ◽  
Antigen Gene

ABSTRACT The 17-kDa antigen of Bartonella henselae has previously been shown to elicit a strong humoral immune response in patients with cat scratch disease (CSD) and to be useful in screening human serum samples for CSD. In this study, PCR amplification of genes homologous to the 17-kDa antigen gene of B. henselae was performed using genomic DNAs from several species ofBartonella, including the currently recognized human pathogens. Amplicons of similar size were demonstrated using the following chromosomal DNA templates: B. henselae (two strains), B. quintana (two strains), B. elizabethae, B. clarridgeiae, B. vinsoniisubsp. vinsonii, and B. vinsonii subsp.berkhoffii. No evidence of a B. bacilliformishomolog of the 17-kDa antigen gene was obtained using multiple primer pairs. DNA sequencing revealed open reading frames capable of coding for proteins with sizes similar to that of the 17-kDa antigen ofB. henselae in all of the amplicons; however, extensive sequence divergence across the genus was noted. Cloning of the amplified products into pUC19 resulted in recombinants that directed synthesis of homologs of the 17-kDa protein. Immunoblot analysis using human sera from CSD cases demonstrated very little cross-reactivity among different species for this protein. In contrast, immunoblots using rabbit serum raised to the recombinant B. henselaeantigen showed extensive cross-reactivity with the proteins of otherBartonella species. The data suggest that the use of the 17-kDa antigen as a serologic reagent may allow the development of more specific diagnostic assays. Furthermore, the nucleotide sequences from the various versions of the 17-kDa antigen gene should be useful for rapid identification of Bartonella at the species level.

scholarly journals Analysis of Bartonella Adhesin A Expression Reveals Differences between Various B. henselae Strains

2006 ◽  
Vol 75 (1) ◽  
pp. 35-43 ◽  
Author(s):  
Tanja Riess ◽  
Günter Raddatz ◽  
Dirk Linke ◽  
Andrea Schäfer ◽  
Volkhard A. J. Kempf
Keyword(s):  
Bartonella Henselae ◽  
Matrix Proteins ◽  
Peliosis Hepatis ◽  
Cat Scratch Disease ◽  
Head Region ◽  
Membrane Anchor ◽  
Bacillary Angiomatosis ◽  
Frameshift Deletion ◽  
Genetic Modifications ◽  
Head Neck

ABSTRACT Bartonella henselae causes cat scratch disease and the vasculoproliferative disorders bacillary angiomatosis and peliosis hepatis in humans. One of the best known pathogenicity factors of B. henselae is Bartonella adhesin A (BadA), which is modularly constructed, consisting of head, neck/stalk, and membrane anchor domains. BadA is important for the adhesion of B. henselae to extracellular-matrix proteins and endothelial cells (ECs). In this study, we analyzed different B. henselae strains for BadA expression, autoagglutination, fibronectin (Fn) binding, and adhesion to ECs. We found that the B. henselae strains Marseille, ATCC 49882, Freiburg 96BK3 (FR96BK3), FR96BK38, and G-5436 express BadA. Remarkably, BadA expression was lacking in a B. henselae ATCC 49882 variant, in strains ATCC 49793 and Berlin-1, and in the majority of bacteria of strain Berlin-2. Adherence of B. henselae to ECs and Fn reliably correlated with BadA expression. badA was present in all tested strains, although the length of the gene varied significantly due to length variations of the stalk region. Sequencing of the promoter, head, and membrane anchor regions revealed only minor differences that did not correlate with BadA expression, apart from strain Berlin-1, in which a 1-bp deletion led to a frameshift in the head region of BadA. Our data suggest that, apart from the identified genetic modifications (frameshift deletion and recombination), other so-far-unknown regulatory mechanisms influence BadA expression. Because of variations between and within different B. henselae isolates, BadA expression should be analyzed before performing infection experiments with B. henselae.

Other bacterial diseasesCat-scratch disease

2011 ◽  
Author(s):  
Michel Drancourt
Keyword(s):  
Lymph Nodes ◽  
Bartonella Henselae ◽  
Severe Disease ◽  
Formal Description ◽  
Cat Scratch Disease ◽  
Bacillary Angiomatosis ◽  
Children And Young Adults ◽  
Eye Infection ◽  
Rough Play ◽  
Spleen Enlargement

Cat-scratch disease (CSD) is a worldwide zoonoses caused by infection with the bacterium, Bartonella henselae. The formal description of the disease by Debré in 1950 (Debré et al. 1950) corresponds to the most frequently diagnosed form of the disease. Cats are the main reservoir for B. henselae and transmission is via Ctniocephalides felis. Humans usually become infected after being scratched or bitten by a cat and is most frequently seen in children and young adults.CSD is a self-limiting illness which often begins with a small papule developing at the site of cat scratch or bite within 3-14 days of the infection. Nearby lymph nodes, usually neck, axillary or groin, become swollen and can persist for several months. It may take up to 7 weeks for the enlarged lymph nodes to appear and individuals may not recall any cat scratch or bite. In healthy cases antibiotics are not indicated.About 5-10% of patients may develop other forms of CSD including eye infection characterised by conjunctivitis and swollen lymph nodes, rash, liver and spleen enlargement, and more rarely encephalitis. Immunosuppresed patients may develop more severe disease, such as bacillary angiomatosis.General advice for preventing CSD includes avoiding rough play with cats, particularly kittens. Cat scratches and bites should be washed immediately with water and soap and cats should not be allowed to lick open wounds.

scholarly journals Comparison of In-House and Commercial Slides for Detection by Immunofluorescence of Immunoglobulins G and M against Bartonella henselae and Bartonella quintana

2002 ◽  
Vol 9 (5) ◽  
pp. 1004-1009 ◽  
Author(s):  
M. Maurin ◽  
J. M. Rolain ◽  
D. Raoult
Keyword(s):  
Bartonella Henselae ◽  
Fluorescent Antibody ◽  
The Other ◽  
Cat Scratch Disease ◽  
Indirect Fluorescent Antibody ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Bartonella Quintana ◽  
Antibody Tests ◽  
Higher Sensitivity

ABSTRACT We compared the sensitivities and specificities of indirect fluorescent antibody tests developed in our laboratory and commercially available from Focus Technologies (FT; formerly MRL Diagnostic) for detection of serum antibodies to Bartonella spp. Serum samples tested were from patients with culture- or PCR-confirmed Bartonella quintana or B. henselae infections causing cat scratch disease (CSD), chronic bacteremia, or endocarditis. At a cutoff titer of 64, the FT test had higher sensitivity than our in-house test in detecting anti-B. henselae immunoglobulin G (IgG) antibodies in CSD patients (91.2 versus 52.9%; P < 0.001). The specificity in serum samples from 85 control patients was, however, lower with the FT test (87%) than with the in-house test (98.8%) (P = 0.002). A cutoff titer of 128 improves the specificity for the FT test but lowers the sensitivity to 85%. For patients infected with B. henselae, our in-house test, but not the FT test, enabled endocarditis to be detected more reliably. With the in-house test, titers of IgG against B. henselae of ≥1,024 were found only in endocarditis patients and not in CSD patients. With the FT test, 19.1% of CSD patients had titers of IgG against B. henselae of ≥1,024 (P < 0.001). Our in-house technique also improved detection of anti-B. quintana antibodies in homeless patients with endocarditis. IgG titers of ≥1,024 were present in 75% of serum samples, but only in 16.7% of serum samples with the FT test (P = 0.004). Since each test has advantages over the other, the serological diagnosis of Bartonella infections would benefit if both tests were used concurrently.

scholarly journals Bartonella henselae Persistence within Mesenchymal Stromal Cells Enhances Endothelial Cells Activation and Infectability Amplifying the Angiogenic Process.

2021 ◽  
Author(s):  
Scutera Sara ◽  
Mitola Stefania ◽  
Sparti Rosaria ◽  
Salvi Valentina ◽  
Grillo Elisabetta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Cytokine Production ◽  
Bartonella Henselae ◽  
Antimicrobial Properties ◽  
Cat Scratch Disease ◽  
Angiogenic Potential ◽  
Bacillary Angiomatosis

Some bacterial pathogens can manipulate the angiogenic response, suppressing or inducing it for their own ends. In humans, B. henselae is associated with cat-scratch disease and vasculoproliferative disorders such as bacillary angiomatosis and bacillary peliosis. Although endothelial cells (ECs) support the pathogenesis of Bartonella , the mechanisms by which Bartonella induces EC activation are not completely clear, as well as the possible contribution of other cells recruited at the site of infection. Mesenchymal stromal cells (MSCs) are endowed with angiogenic potential and play a dual role in infections exerting antimicrobial properties but also acting as a shelter for pathogens. Here we delved into the role of MSCs as reservoir of Bartonella and modulator of EC functions. B. henselae readily infected MSCs and survived in perinuclear bound vacuoles for up to 8 days. Infection enhanced MSC proliferation and the expression of EGFR, TLR2 and NOD1, proteins that are involved in bacterial internalization and cytokine production. Secretome analysis revealed that infected MSCs secreted higher levels of the proangiogenic factors VEGF, FGF-7, MMP-9, PIGF, serpin E1, TSP-1, uPA, IL-6, PDGF-D, CCL5 and CXCL8. Supernatants from B. henselae -infected MSCs increased the susceptibility of ECs to B. henselae infection and enhanced EC proliferation, invasion and reorganization in tube-like structures. Altogether, these results candidate MSCs as a still underestimated niche for B. henselae persistent infection and reveal a MSC-EC crosstalk that may contribute to exacerbate bacterial-induced angiogenesis and granuloma formation.

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