Increased Leukocyte Thromboplastin Activity by Sensitized Lymphocytes: A Link Between Immunology and Blood Coagulation

1979 ◽  
Author(s):  
W.G. van Aken ◽  
W.P. Zeylemaker ◽  
E.A.M. Stricker ◽  
J.I.H. Oh ◽  
C.J.W. van Ginkel
Keyword(s):  
Cell Suspension ◽  
Immune Reaction ◽  
Specific Antigen ◽  
Mononuclear Leukocytes ◽  
Immune Stimulation ◽  
Mixed Leukocyte Reaction ◽  
Fibrin Deposition ◽  
Low Responders ◽  
High Responders ◽  
Stimulation Of

Fibrin deposition and infiltration of mononuclear leukocytes (MN) frequently occurs concomitantly at the site of an Immune reaction. As human MN can produce thromboplastin (TP) we have investigated whether immune stimulation of MN can trigger the production of TP by these cells. Both specific antigen (tuberculin PPD) and allogeneic lymphocytes were used for stimulation. MN from l4 donors with a high response to PPD and from 11 donors with a low response to PPD (based on the incorporation of 3H-thymidine) were cultured for 7 days in the presence or absence of PPD and the TP activity of the whole cell suspension was determined. When PPD was absent, TP levels in MN from low and high responders were not different (113±47 and 197±111 u/ml resp. p>0.10). With PPD, however, MN from high responders produced 1470±413 u TP/ml compared to 126±49 u TP/ml produced by MN from low responders (p<0.005). Similar results were obtained when MN were kept in culture for either 4 or 11 days. However, after 24 hours in culture» TP synthesis was not different in the MN of the 2 groups. When MN were cultured in a mixed leukocyte reaction, allogeneic MN produced more TP (405±151 u/ml) than control cultures (95±21 u/ml) (p<0.025;n=22). These data indicate that stimulated lymphocytes can enhance TP actiyity of leukocytes. It is postulated that fibrin formation In immune reactions such as delayed hypersensitivity and rejection of kidney allografts Is mediated by immunologically stimulated MN.

Increased Leukocyte Thromboplastin Activity by Sensitized Lymphocytes: A Link Between Immunology and Blood Coagulation

1979 ◽  
Author(s):  
W van Aken ◽  
W Zeylemaker ◽  
E Stricker ◽  
J Oh ◽  
C van Ginkel
Keyword(s):  
Cell Suspension ◽  
Immune Reaction ◽  
Specific Antigen ◽  
Mononuclear Leukocytes ◽  
Immune Stimulation ◽  
Mixed Leukocyte Reaction ◽  
Fibrin Deposition ◽  
Low Responders ◽  
High Responders ◽  
Stimulation Of

Fibrin deposition and infiltration of mononuclear leukocytes (MN) frequently occurs concomitantly at the site of an immune reaction. As human MN can produce thromboplastin (TP) we have investigated whether immune stimulation of MN can trigger the production of TP by these cells. Both specific antigen (tuberculin PPD) and allogeneic lymphocytes were used for stimulation. MN from 14 donors with a high response to PPD and from 11 donors with a low response to PPD (based on the incorporation of 3H-thymidine) were cultured for 7 days in the presence or absence of PPD and the TP activity of the whole cell suspension was determined. When PPD was absent, TP levels in MN from low and high responders were not different (113±47 and 197±111 u/ml resp. p ˃ 0.10). With PPD, however, MN from high responders produced 1470±413 u TP/ml compared to 126±49 u TP/ml produced by MN from low responders (p < 0.005). Similar results were obtained when MN were kept in culture for either 4 or 11 days. However, after 24 hours in culture, TP synthesis was not different in the MN of the 2 groups. When MN were cultured in a mixed leukocyte reaction, allogeneic MN produced more TP (405±151 u/ml) than control cultures (95±21 u/ml) (p<0.025;n=22). These data indicate that stimulated lymphocytes can enhance TP activity of leukocytes. It is postulated that fibrin formation in immune reactions such as delayed hypersensitivity and rejection of kidney allografts is mediated by immunologically stimulated MN.

Management of Haemophilia A with Antibodies - The Effect of Combined Treatment with Factor VIII, Hydrocortisone and Cyclophosphamide

1985 ◽  
Vol 54 (04) ◽  
pp. 776-779 ◽  
Author(s):  
U Hedner ◽  
L Tengborn
Keyword(s):  
Factor Viii ◽  
Combined Treatment ◽  
Factor Ix ◽  
Treatment Schedule ◽  
High Antibody ◽  
Anamnestic Response ◽  
Short Intervals ◽  
Low Responders ◽  
Antibody Levels ◽  
High Responders

SummaryImmune tolerance has by several methods been induced in haemophiliacs with antibodies. A conversion of “high responders” into “low responders” was previously reported after repeated moderate factor IX doses over periods of 7-10 days in combination with cyclophosphamide and steroids in two patients with haemophilia B and inhibitors. This paper reports similar results in a heamophilia A patient by giving factor VIII, cyclophosphamide, and steroids during relatively short periods of time (7-8 days). The anamnestic response markedly decreased already following the first treatment and never exceeded a level of 1 u/ml (˜ 3 BU/ml) even when boosted with ordinary factor VIII doses for only 3 days. It is concluded that the markedly decreased secondary antibody response is most probably the result of factor VIII given at short intervals (twice a day) for periods of up to about one week when given in combination with cyclophosphamide and steroids. The same effect may be achieved by other methods. The treatment schedule suggested in the present paper is, however, simple and avoids long periods of high antibody levels. Furthermore, the total factor VIII dose used is lower than suggested in most other treatment schedules, which makes the treatment substantially less expensive.

scholarly journals Specific and Nonspecific Immune Stimulation of MHC-II-Deficient Mice Results in Chronic HSV-1 Infection of the Trigeminal Ganglia Following Ocular Challenge

Virology ◽  
1999 ◽  
Vol 261 (2) ◽  
pp. 367
Author(s):  
Homayon Ghiasi ◽  
Guey-Chuen Perng ◽  
Florence M. Hofman ◽  
Steve Cai ◽  
Anthony B. Nesburn ◽  
...  
Keyword(s):  
Trigeminal Ganglia ◽  
Immune Stimulation ◽  
Mhc Ii ◽  
Deficient Mice ◽  
Stimulation Of ◽  
Hsv 1

scholarly journals Perspectives approach to the assessment of the quality of the vaccine plague live in terms of immunogenicity. Epidemiology and Vaccinal Prevention

2019 ◽  
Vol 18 (1) ◽  
pp. 50-54
Author(s):  
S. E. Gostischeva ◽  
N. V. Abzaeva ◽  
E. L. Rakitina ◽  
D. G. Ponomarenko ◽  
M. V. Kostuchenko ◽  
...  
Keyword(s):  
Specific Antigen ◽  
Water Soluble ◽  
Control Group ◽  
In Vitro Tests ◽  
Laboratory Mice ◽  
Early Activation ◽  
High Degree ◽  
Stimulation Of

Research objective–studying of a possibility of application antigen – stimulated cellular in vitro tests and technology of the cytometric analysis for control of immunogene activity of batches of vaccine plague live.Materials and methods.As biomodels used white laboratory mice, immunized commercial medicine of vaccine of the plague NIIEG line, live from a strain of Yersinia pestis EV, in doses – 8 х 102, 4 х 103, 2 х 104 and 1 х 105 of living microbic cells. Blood for a research was taken from intact mice and on 7, 14 and 21 days after immunization. The intensity of an antigenreaktivnost of lymphocytes was defined in cellular in vitro tests, analyzing a marker of early activation (CD45+CD3+CD25+) of lymphocytes with use of the monoclonal antibodies conjugated from fluorokhroma. As specific antigen used a complex of water-soluble antigens of a plague microbe.Results.As a result of a research it is shown that at the animals vaccinated by doses 4 х 103 – 1 х 105 living microbic cells, the highest level of an expression activation marker lymphocytes at anti-gene stimulation of in vitro is registered on 14 days after immunization, at the same time the quantity of CD25 – positive lymphocytes are on average 6.8 times higher, than in control group. High degree of direct link (coefficient of correlation of r = 1,000) quantities of the survived animals with increase in level of lymphocytes, expressiruyushchy markers of early activation – CD25 is established.Conclusions.The offered technique can be used as the additional test when studying degree of immunogenicity of new (kandidatny) vaccines against plague.

scholarly journals Specific and Nonspecific Immune Stimulation of MHC-II-Deficient Mice Results in Chronic HSV-1 Infection of the Trigeminal Ganglia Following Ocular Challenge

Virology ◽  
1999 ◽  
Vol 258 (2) ◽  
pp. 208-216 ◽  
Author(s):  
Homayon Ghiasi ◽  
Guey-Chuen Pemg ◽  
Florence M. Hofman ◽  
Steve Cai ◽  
Anthony B. Nesburn ◽  
...  
Keyword(s):  
Trigeminal Ganglia ◽  
Immune Stimulation ◽  
Mhc Ii ◽  
Deficient Mice ◽  
Stimulation Of ◽  
Hsv 1
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