Antiviral Activity of Natural Sulphated Galactans on Herpes Virus Multiplication in Cell Culture

Planta Medica ◽  
1997 ◽  
Vol 63 (05) ◽  
pp. 429-432 ◽  
Author(s):  
María Carlucci ◽  
Luis Scolaro ◽  
María Errea ◽  
María Matulewicz ◽  
Elsa Damonte
1970 ◽  
Vol 7 (5) ◽  
pp. 448-454 ◽  
Author(s):  
Alfred G. Karlson ◽  
Herman R. Seibold ◽  
Robert H. Wolf

Mycobacterium abscessus was isolated from the lungs of an owl monkey which died 27 days after intraperitoneal injection of herpes virus-infected Vero cells. The lungs and liver had multiple microscopic granulomas with acid-fast microorganisms. The mycobacteria also were isolated from a Vero-cell culture inoculated with a suspension of lung and liver. The same microorganism was eventually isolated from Vero cells of the same source as that used to propagate the herpes virus for the original attempt to infect the monkey.


Biodiscovery ◽  
2017 ◽  
Vol 20 ◽  
pp. e15022
Author(s):  
Petya Angelova ◽  
Venelin Tsvetkov ◽  
Anton Hinkov ◽  
Daniel Todorov ◽  
Kalina Shishkova ◽  
...  

2014 ◽  
Vol 9 (1) ◽  
pp. 41
Author(s):  
Lila Gardenia ◽  
Isti Koesharyani

Primary cell culture from tail epidermal tissue of koi carp (Cyprinus carpio koi) was developed. Cells were grown in Leibovits-15 medium supplemented with 20% fetal bovine serum and antibiotics (Penicillin/Streptomycin and Kanamycin). Cell growth was observed in a range of incubation temperature (17oC±2oC, 22oC±2oC, 27oC±2oC, and 32oC±2oC) in order to determine the optimum temperature. The cells were able to grow at a range of temperature between 17oC to 32oC with optimal growth at 22oC. Primary cells infected with koi herpes virus produced typical cytopathic effects characterized by severe vacuolation and deformation of nuclei, which is consistent with those of previous reports. Artificial injection experiment by using supernatant koi herpes virus SKBM-1 isolate revealed that it could cause 90% mortality in infected fish within two weeks. PCR test with Sph I-5 specific primers carried out with DNA template from supernatant virus, pellet cell, and gills of infected fish showed positive results in all samples (molecular weight of DNA target 290 bp). The cells were found to be susceptible to koi herpes virus and can be used for virus propagation.


1995 ◽  
Vol 26 (3) ◽  
pp. A326 ◽  
Author(s):  
D. Reymen ◽  
L. Naesens ◽  
J. Balzarini ◽  
A. Holy ◽  
E. De Clercq

1999 ◽  
Vol 54 (1-2) ◽  
pp. 75-83 ◽  
Author(s):  
Ivanka G. Stankova ◽  
Mario F. Simeonov ◽  
Vera Maximova ◽  
Angel S. Galabov ◽  
Evgeny V. Golovinsky

New 3’-, 5’-, 5-bromo-2’-deoxyuridine (3 a-g) and 3’-, 5’-thymidine (4 a-i) analogues with amino acid and peptide residues were synthesized and evaluated for antiviral activity. The influence of long peptide chains, essential amino acids and the effect of this structural modification on the antiviral activity has been also reported. Three 5-bromo-2’-deoxyuridine derivatives containing glycyl-, glycyl-glycyl-and glycyl-gly-cyl-glycyl-residues (3a, 3b, 3c) showed a strong activity against the herpes virus PsRV and a moderate one vs. HSV-1. The corresponding thymidine analogues were considerably less effective, and only compounds 4d and 4h showed a borderline effect against PsRV


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