Studies on the Biochemistry and Molecular Biology of Peroxidase Isoenzymes in Plants and Cell Suspension Cultures ofLupinus polyphyllus

Planta Medica ◽  
1990 ◽  
Vol 56 (06) ◽  
pp. 607-607
Author(s):  
R. Perrey ◽  
M. Wink
Keyword(s):  
Molecular Biology ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes

The effect of 2,4-D and kinetin on peroxidase activity and isoenzyme pattern in cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1976 ◽  
Vol 54 (16) ◽  
pp. 1857-1867 ◽  
Author(s):  
Paul G. Arnison ◽  
W. G. Boll
Keyword(s):  
Phaseolus Vulgaris ◽  
Cell Suspension ◽  
Growth Regulators ◽  
Peroxidase Activity ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Cotyledon Cell ◽  
Cell Growth And Differentiation ◽  
Dichlorophenoxy Acetic Acid

Cotyledon cell suspension cultures of Phaseolus vulgaris were grown in the presence and absence of the growth regulators (2,4-dichlorophenoxy)acetic acid (2,4-D) and kinetin. Peroxidase (EC 1.11.1.7) activity was at a minimum during the phase of cell division and at a maximum during the phase of cell expansion. Both the pattern and activity of peroxidase isoenzymes changed during the culture cycle.Cells cultured without growth regulators showed increased peroxidase activity and changed isoenzyme patterns. Certain peroxidase isoenzymes were only present or prominent during specific phases of the culture cycle.The electrophoretic mobilities of peroxidase isoenzymes detected in the medium were not the same as those of the cytoplasmic isoenzymes. Cell cultures grown with and without growth regulators showed different patterns of medium peroxidase activity.Results are discussed in relation to the correlation of peroxidase activity with cell wall expansion and the possible role of peroxidase in cell growth and differentiation.

Cellular and Subcellular Localization of Peroxidase Isoenzymes in Plants and Cell Suspension Cultures from Lupinus polyphyllus

1989 ◽  
Vol 44 (11-12) ◽  
pp. 931-936 ◽  
Author(s):  
Ralf Perrey ◽  
Marie-Theres Hauser ◽  
Michael Wink
Keyword(s):  
Subcellular Localization ◽  
Cell Suspension ◽  
Intercellular Space ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Peroxidase Isoenzymes ◽  
Lupinus Polyphyllus ◽  
Minor Degree ◽  
A Minor

Abstract , leaf protoplasts and cell suspension cultures of Lupinus polyphyllus and isolated vacuoles were studied for cellular and subcellular localization of peroxidase isoenzymes. Isoelectric focusing revealed 16 peroxidase isoenzymes. The basic peroxidase isoenzymes are predominantly localized in the vacuole and, to a minor degree, unbound in the intercellular space. The acidic isoenzymes are cell wall-bound in plants and not detectable in suspension-cultured cells. Large amounts (up to 11.0 U/ml) of a single basic isoenzyme are detectable in the spent medium of cell suspension cultures.

Quantitative analysis of hinokiol from cell suspension cultures of Cephalotaxus fortunei

2011 ◽  
Vol 29 (6) ◽  
pp. 567-570
Author(s):  
Xiaohui XU ◽  
Wei ZHANG ◽  
Changhong YAO ◽  
Xupeng CAO ◽  
Song XUE
Keyword(s):  
Quantitative Analysis ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures
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