The Effect ofIn VivoThyroxine Treatment on Insulin Receptors, Glucose Transport and GLUT4 in Rat Adipocytes

1997 ◽  
Vol 29 (01) ◽  
pp. 16-19 ◽  
Author(s):  
M. Fickova ◽  
S. Zorad ◽  
L. Macho
Keyword(s):  
Glucose Transport ◽  
Insulin Receptors ◽  
Rat Adipocytes

scholarly journals Mechanisms of the ability of insulin to activate the glucose-transport system in rat adipocytes

1978 ◽  
Vol 172 (1) ◽  
pp. 137-145 ◽  
Author(s):  
J M Olefsky
Keyword(s):  
Plasma Membrane ◽  
Glucose Transport ◽  
Lateral Diffusion ◽  
Insulin Receptors ◽  
Activation Energies ◽  
Intrinsic Activity ◽  
Rat Adipocytes ◽  
Plasma Membrane Fluidity ◽  
Glucose Transport System ◽  
Km Value

Isolated rat adipocytes were used to assess the mechanisms of the ability of insulin to accelerate glucose transport. Glucose transport was determined by measuring the initial rates of 2-deoxyglucose uptake, and at 24 degrees C insulin increased the Vmax. of transport from 7.3 +/- 1 to 23.1 +/- 2 nmol/min per 10(6) cells, but the Km value remained unchanged (2.5, cf. 2.4 mM). When the Vmax. of basal and insulin-stimulated transport was measured as a function of temperature (15-37 degrees C), parallel Arrhenius plots were obtained yielding equal activation energies of approx. 59kJ/mol. Since both processes have equal activation energies the data indicate that insulin increases Vmax. by increasing the number of available carriers rather than enhancing intrinsic activity of already functioning carriers. Since the ability of insulin to activate glucose transport did not decrease with temperature (whereas plasma-membrane fluidity declines), it is suggested that lateral diffusion of insulin receptors within the plasma-membrane bilayer is not a rat-determining step in insulin action.

Degradation of insulin receptors in rat adipocytes

Diabetes ◽  
1983 ◽  
Vol 32 (11) ◽  
pp. 1001-1009 ◽  
Author(s):  
K. A. Heidenreich ◽  
P. Berhanu ◽  
D. Brandenburg ◽  
J. M. Olefsky
Keyword(s):  
Insulin Receptors ◽  
Rat Adipocytes

Degradation of Insulin Receptors in Rat Adipocytes

Diabetes ◽  
1983 ◽  
Vol 32 (11) ◽  
pp. 1001-1009 ◽  
Author(s):  
K. A. Heidenreich ◽  
P. Berhanu ◽  
D. Brandenburg ◽  
J. M. Olefsky
Keyword(s):  
Insulin Receptors ◽  
Rat Adipocytes

Effect of purified phospholipases on glucose transport, insulin binding, and insulin action in isolated rat adipocytes

1982 ◽  
Vol 4 (4) ◽  
pp. 261-271 ◽  
Author(s):  
Tj. Wieringa ◽  
G. Bruin ◽  
W. P. M. Meerwijk ◽  
H. M. J. Krans
Keyword(s):  
Glucose Transport ◽  
Insulin Action ◽  
Insulin Binding ◽  
Rat Adipocytes ◽  
Isolated Rat
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