Global analysis of ion dependence unveils hidden steps in DNA binding and bending by integration host factor

2013 ◽  
Vol 139 (12) ◽  
pp. 121927 ◽  
Author(s):  
Paula Vivas ◽  
Yogambigai Velmurugu ◽  
Serguei V. Kuznetsov ◽  
Phoebe A. Rice ◽  
Anjum Ansari
Keyword(s):  
Dna Binding ◽  
Global Analysis ◽  
Integration Host Factor ◽  
Host Factor ◽  
Dna Binding And Bending

scholarly journals Participation of the Flank Regions of the Integration Host Factor Protein in the Specificity and Stability of DNA Binding

1995 ◽  
Vol 270 (30) ◽  
pp. 17902-17907 ◽  
Author(s):  
Laurence Zulianello ◽  
Peter van Ulsen ◽  
Pieter van de Putte ◽  
Nora Goosen
Keyword(s):  
Dna Binding ◽  
Integration Host Factor ◽  
Host Factor

scholarly journals Two-step interrogation then recognition of DNA binding site by Integration Host Factor: an architectural DNA-bending protein

2017 ◽  
Vol 46 (4) ◽  
pp. 1741-1755 ◽  
Author(s):  
Yogambigai Velmurugu ◽  
Paula Vivas ◽  
Mitchell Connolly ◽  
Serguei V Kuznetsov ◽  
Phoebe A Rice ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Site ◽  
Dna Bending ◽  
Integration Host Factor ◽  
Host Factor ◽  
Dna Binding Site

scholarly journals Binding of Escherichia coli integration host factor (IHF) to the origin segment of p15A plasmid.

1995 ◽  
Vol 42 (1) ◽  
pp. 103-108
Author(s):  
E Hiszczyńska-Sawicka ◽  
J Kur
Keyword(s):  
Escherichia Coli ◽  
Consensus Sequence ◽  
Integration Host Factor ◽  
Host Factor ◽  
Mobility Shift ◽  
Consensus Sequences ◽  
Report Data ◽  
Functional Dna ◽  
Gel Mobility Shift ◽  
Dna Binding And Bending

The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data concerning the binding of IHF protein to the plasmid orip15A region. IHF binds to the single site of the DNA fragment containing the orip15A, as shown by the gel mobility shift assays and footprinting experiment. On the basis of the ihf consensus sequences published, we have been able to identify one sequence of putative ihf site into the orip15A sequence with two mismatches in relation to the consensus sequence of Kur et al., 1989, Gene 81, 1-15. One ihf binding site was also found in the oriColE1 region sequence with three mismatches in relation to this consensus sequence.

Mutants of Escherichia coli integration host factor: DNA-binding and recombination properties

Biochimie ◽  
1994 ◽  
Vol 76 (10-11) ◽  
pp. 1030-1040 ◽  
Author(s):  
L.M. Hales ◽  
R.I. Gumport ◽  
J.F. Gardner
Keyword(s):  
Escherichia Coli ◽  
Dna Binding ◽  
Integration Host Factor ◽  
Host Factor
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