Open-Chain Crown-Ether-Derived Two-Photon Fluorescence Probe for Real-Time Dynamic Biopsy of Mercury Ions

2017 ◽  
Vol 70 (6) ◽  
pp. 705
Author(s):  
Chibao Huang ◽  
Daohai Zhang ◽  
Junle Qu ◽  
Xiaonan Liu ◽  
Guanglian Zhao ◽  
...  
Keyword(s):  
Water Solubility ◽  
Fluorescence Probe ◽  
Stokes Shift ◽  
Cell Permeability ◽  
Photon Absorption ◽  
Live Cells ◽  
Open Chain ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A novel two-photon fluorescence probe for Hg2+ derived from bis(styryl)terephthalonitrile, as a two-photon fluorophore, and bis[2-(2-hydroxyethyl sulfanyl) ethyl]amino group (ionophore), as a novel Hg2+ ligand, was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1067 GM) in H2O, non-cytotoxic effect, long wavelength emission at 588 nm, large Stokes shift (121 nm), excellent photostability, high water solubility, good cell permeability, and pH insensitivity in the biologically relevant range. The probe can selectively detect Hg2+ ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant is 8.73 × 105 M–1.

A two-photon off-on fluorescence probe for imaging thiols in live cells and tissues

2016 ◽  
Vol 15 (3) ◽  
pp. 412-419 ◽  
Author(s):  
Xinyue Zhu ◽  
Yan Li ◽  
Wenyan Zan ◽  
Jianjian Zhang ◽  
Zhenjie Chen ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Probe ◽  
Live Cells ◽  
Imaging Probe ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

An effective off-on two-photon fluorescence imaging probe for thiols was developed and successfully applied to image thiols both in live cells and tissues.

Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

2019 ◽  
Vol 91 (22) ◽  
pp. 14691-14696 ◽  
Author(s):  
Liyan Chen ◽  
Myoung Ki Cho ◽  
Di Wu ◽  
Hwan Myung Kim ◽  
Juyoung Yoon
Keyword(s):  
Fluorescence Probe ◽  
Live Cells ◽  
Two Photon ◽  
Selective Monitoring ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Interactions of picosecond laser pulses with organic molecules I. Two-photon fluorescence quenching and singlet states excitation in Rhodamine dyes

1972 ◽  
Vol 328 (1572) ◽  
pp. 97-121 ◽  
Keyword(s):  
Rhodamine 6G ◽  
Second Harmonic ◽  
Random Phase ◽  
Stimulated Emission ◽  
Photon Absorption ◽  
Picosecond Pulses ◽  
Two Photon ◽  
Singlet States ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Intensity dependent quenching and reversal of the two-photon fluorescence patterns in Rhodamine 6G and DPA, of picosecond pulses from a mode-locked ruby laser have been investigated by measurements of the two-photon fluorescence efficiencies of these dyes. While for Rhodamine 6G there was a marked departure from the square law dependence at high laser intensities, the experimental curve for DPA showed no evidence of quenching. When excited by a mode-locked neodymium: glass laser Rhodamine 6G fluorescence was not quenched at fluxes as high as 5 x 10 30 photons cm -2 s -1 but in Rhodamine B quenching appeared at a laser flux of 3 x 10 27 photons cm -2 s -1 . These quenching results and measurements of the absorption of pulses by Rhodamine 6G, previously excited by second harmonic pulses, are explained by the effects of single photon absorption and stimulated emission from the S 1 and S 2 excited singlet states. A square pulse approximation has been employed to solve the general rate equations and the fitting of the calculated curves to the experimental results gave values for the stimulated emission and absorption cross-sections of the S 1 and S 2 states of Rhodamine 6G and for the relaxation time (~ 2 ps) between the vibrational manifolds of these excited states. Taking into account random phase and amplitude fluctuations of the picosecond pulses, time and space averaged two-photon fluorescence profiles, using these values of the dye parameters, showed quenching and reversal of the patterns for the laser pulse intensities at which these effects were experimentally observed. The possibilities of frequency tunable pulses, of transform-limited durations, from mode-locked dye lasers employed with an electro-optical streak camera of time-resolution equal to that of the pulse durations (~ 2 ps) for time-resolved excited state molecular spectroscopy are briefly considered.

Imaging of formaldehyde fluxes in epileptic brains with a two-photon fluorescence probe

2020 ◽  
Vol 56 (27) ◽  
pp. 3871-3874
Author(s):  
Jian Chen ◽  
Chenwen Shao ◽  
Xueao Wang ◽  
Jin Gu ◽  
Hai-Liang Zhu ◽  
...  
Keyword(s):  
Animal Models ◽  
Fluorescence Probe ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Epigenetic Processes

A two-photon (TP) fluorescence probe has been developed for imaging endogenous FA fluxes during metabolic and epigenetic processes in animal models, especially in live brains.

A Red Emissive Two‐Photon Fluorescence Probe Based on Carbon Dots for Intracellular pH Detection

Small ◽  
2019 ◽  
Vol 15 (48) ◽  
pp. 1901673 ◽  
Author(s):  
Xiaoxue Ye ◽  
Yunhui Xiang ◽  
Qirong Wang ◽  
Zhen Li ◽  
Zhihong Liu
Keyword(s):  
Intracellular Ph ◽  
Carbon Dots ◽  
Fluorescence Probe ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Ph Detection
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