Effect of temperature on seed set and in vitro pollen germination in French beans (Phaseolus vulgaris)

1979 ◽  
Vol 19 (101) ◽  
pp. 725 ◽  
Author(s):  
PJ Farlow ◽  
DE Byth ◽  
NS Kruger
Keyword(s):  
Pollen Germination ◽  
Seed Set ◽  
Germination Percentage ◽  
French Bean ◽  
Hot Water ◽  
Effect Of Temperature ◽  
In Vitro Pollen Germination ◽  
French Beans ◽  
Different Temperatures

A technique for in vitro germination of French bean pollen was developed and the effect of temperature on gamete development, pollen germination and seed set investigated. Temperature had a profound effect on in vitro pollen germination percentage, pollen tube growth and bursting percentage. These were maximal at 7.2�C, 16.7�C and 38.3�C, respectively. In this study, pollen development was not affected at day/night temperatures of 16.1�/12.8�C, and ovule abortion was the cause of seed set failure at these temperatures. Hot water treatment (48�-44�C) of flowers caused failure of seed set due to pollen inviability. Consequently this technique may allow hybridization without emasculation in beans. Treatment of buds with hot water of different temperatures and in vitro pollen germination at high temperatures may have application as screening techniques for heat tolerance in French beans.

scholarly journals The effect of temperature on pollen germination and pollen tube growth of pear cultivars

2016 ◽  
Vol 61 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Aleksandar Radovic ◽  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Dejan Djurovic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Different Temperatures ◽  
Pollen Tube Length

The effect of three different temperatures (8, 16 and 24?C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars (?Butirra Precoce Morettini?, ?Williams?, ?Conference? and ?Abate Fetel?). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24?C (53.25%), somewhat lower at 16?C (44.72%) and the lowest at 8?C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24?C compared to 8?C. The temperature of 8?C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24?C were optimal for pollen germination and pollen tube growth.

scholarly journals Effects of Ca2+ on in vitro pollen germination of three Acacia species

2016 ◽  
Vol 65 (2) ◽  
pp. 11-16 ◽  
Author(s):  
Ni Zhan ◽  
Liejian Huang
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Acacia Mangium ◽  
Germination Percentage ◽  
Tube Growth ◽  
Acacia Auriculiformis ◽  
Tube Length ◽  
In Vitro Pollen Germination

Abstract We investigated the effects of Ca2+ on in vitro pollen germination of Acacia auriculiformis, Acacia mangium, and Acacia crassicarpa under different concentrations of Ca2+ (50 mg.L−1, 100 mg.L−1, 150 mg.L−1, 200 mg.L−1, 250 mg.L−1, 300 mg.L−1, 350 mg.L−1 and 400 mg.L−1) in the present study. Our results revealed that Ca2+ could stimulate the pollen germination percentage and the pollen tube growth of A. auficuliformis and A. crassicarpa under a certain concentration. In vitro pollen germination of A. mangium did not require exogenous Ca2+. With high Ca2+ concentration, the pollen germination of A. auriculiformis, A. mangium, and A. crassicarpa were obviously inhibited. With the rise of Ca2+ concentration, the percentage of pollen germination, the pollen tube length, the pollen tube growth, and the number of pollen tube were increased. However, the pollen germination percentage decreased with high Ca2+ concentration.

scholarly journals Irradiation and Heat Affect Peach Pollen Germination and Fertility

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 290-291 ◽  
Author(s):  
M.R. Pooler ◽  
R. Scorza
Keyword(s):  
Pollen Germination ◽  
Interspecific Hybrids ◽  
Prunus Persica ◽  
Seed Set ◽  
Germination Capacity ◽  
Male Sterile ◽  
In Vitro Pollen Germination ◽  
Low Levels ◽  
Parthenogenetic Egg

Pollen from the doubled haploid peach [Prunus persica (L.) Batsch] `Hall-D' was irradiated with 0, 290, 530, 820, 1000, 5000, or 9000 Gray (Gy) of gamma radiation, 113 μW·cm-2 of ultraviolet (UV) radiation, or exposed to 100 °C for 2 h. In vitro pollen germination percentages were recorded and pollen was used to pollinate more than 10,000 emasculated or male-sterile peach flowers. Although pollen germination in vitro was stimulated by <1000 Gy of gamma irradiation, seed set following pollination was greatly reduced in all treatments. These results suggest that low levels of irradiation are sufficient to render pollen infertile while still maintaining germination capacity. Such results may be useful for pollination-induced parthenogenetic egg division for the production of maternally derived haploids and for the production of interspecific hybrids.

scholarly journals The effect of temperature on pollen germination and pollen tube growth of sour cherry cultivars

2014 ◽  
Vol 59 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Dragan Milatovic ◽  
Dragan Nikolic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Different Temperatures ◽  
Influence Of Temperature On

The study was carried out to determine the effect of three different temperatures (5, 15 and 25?C) on in vitro pollen germination and pollen tube growth of five sour cherry cultivars: ?Heimanns Konservenweichsel?, ?Kelleriis 14?, ?Oblacinska?, ?Rexelle? and ?Sumadinka?. Pollen germination a % agar % Temperature significantly affected pollen germination. High germination rates (50-70%) were obtained at both 15?C and 25?C. Satisfactory germination rates (42-51%) were also obtained at 5?C in some cultivars (?Rexelle?, ?Sumadinka? and ?Heimanns Konservenweichsel?). The influence of temperature on the pollen tube growth was more prominent. The length of pollen tubes was three to six times higher at 15?C and 25?C in comparison with 5?C. This has led to the conclusion that the temperature of 5?C, although it could be adequate for pollen germination, is not high enough for optimal pollen tube growth. was determined by germinating pollen grains in culture medium containing 0.7agar-and 15sucrose.

scholarly journals Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aiyan Guan ◽  
Inge Van Damme ◽  
Frank Devlieghere ◽  
Sarah Gabriël
Keyword(s):  
Enzymatic Digestion ◽  
Infected Fish ◽  
Effect Of Temperature ◽  
Video Recordings ◽  
Different Temperatures ◽  
Semi Solid ◽  
Zoonotic Parasites ◽  
The Impact ◽  
Phosphate Buffered Saline

AbstractAnisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2 and O2) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO2 and O2 with N2 as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for Anisakis spp. larvae and 37 ℃ for Pseudoterranova spp. larvae. During the first 10 min, the median migration of Anisakis spp. larvae was 10 cm at 22 ℃, and the median migration of Pseudoterranova spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO2 or O2 conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for Anisakis spp. and Pseudoterranova spp., while CO2 and O2 did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.

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