Population structure of pink ling (Genypterus blacodes) from south-eastern Australian waters, inferred from allozyme and microsatellite analyses

2001 ◽  
Vol 52 (7) ◽  
pp. 965 ◽  
Author(s):  
R. D. Ward ◽  
S. A. Appleyard ◽  
R. K. Daley ◽  
A. Reilly

Thirty eight allozyme loci were examined in orange and pink morphs of pink ling (Genypterus blacodes) and in rock ling (G. tigerinus). Six of the loci were species-diagnostic. No differences were observed between the orange and pink morphs, which appear to be the juvenile and adult forms respectively of pink ling. The pink ling forms an important component of Australia’s South-East Fishery and is managed as a single stock. Three polymorphic allozyme loci (average heterozygosity 0.324) and nine polymorphic microsatellite loci (average heterozygosity 0.823) were examined in collections of pink ling from five regions of the fishery. Estimates of the extent of population subdivision were effectively zero (F ST values per locus ranging from 0.0052 to –0.0065, P values non-significant), and the null hypothesis of a single pink ling stock in the South East Fishery could not be rejected.

1996 ◽  
Vol 44 (3) ◽  
pp. 331 ◽  
Author(s):  
M Byrne ◽  
MI Marquezgarcia ◽  
T Uren ◽  
DS Smith ◽  
GF Moran

Four microsatellite loci have been characterised in Eucalyptus nitens Maiden and in six other eucalypt species. The dinucleotide repeats were identified by screening a Sau3AI genomic DNA library from E. nitens with (CA)n and (GA)n oligonucleotide probes and sequencing the positive clones. Genetic analysis of 20 unrelated individuals from five populations of E. nitens showed all loci to be highly polymorphic with an average of 9.5 alleles per locus and an average heterozygosity of 0.575. Analysis of four individuals from each of six species from three subgenera showed complete conservation of microsatellite loci between species within the same subgenus, Symphyomyrtus, and conservation of 50% of loci across species between the two main subgenera, Symphyomyrtus and Monocalyptus. None of the primers amplified microsatellite loci in Eucalyptus maculata from the subgenus Corymbia. All microsatellite loci that were detected were polymorphic. Highly polymorphic microsatellite loci that are conserved across species will be useful for mapping quantitative traits, fingerprinting breeding lines, and for within-population studies requiring fine-scale analysis of genetic variation.


2015 ◽  
Vol 81 (3) ◽  
pp. 407-411
Author(s):  
Melissa K. Holborn ◽  
Madoka V. Krick ◽  
Stephanie Pedersen ◽  
David A. G. A. Hunt ◽  
Elizabeth G. Boulding

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