Steroids affect gene expression, ciliary activity, glucose uptake, progesterone receptor expression and immunoreactive steroidogenic protein expression in equine oviduct explants in vitro

The oviduct undergoes dramatic functional and morphological changes throughout the oestrous cycle of the mare. To unravel the effects of steroids on the morphology, functionality and gene expression of the equine oviduct, an in vitro oviduct explant culture system was stimulated with physiological concentrations of progesterone and 17β-oestradiol. Four conditions were compared: unsupplemented preovulatory explants, preovulatory explants that were stimulated with postovulatory hormone concentrations, unsupplemented postovulatory explants and postovulatory explants that were stimulated with preovulatory hormone concentrations. The modulating effects of both steroids on oviduct explants were investigated and the following parameters examined: (1) ciliary activity, (2) glucose consumption and lactate production pattern, (3) ultrastructure, (4) mRNA expression of embryotrophic genes, (5) steroidogenic capacities of oviductal explants and (6) progesterone receptor expression. The present paper shows that the equine oviduct is an organ with potential steroidogenic capacities, which is highly responsive to local changes in progesterone and 17β-oestradiol concentrations at the level of morphology, functionality and gene expression of the oviduct. These data provide a basis to study the importance of endocrine and paracrine signalling during early embryonic development in the horse.

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Antagonism between estrogens and androgens on gcdfp-15 gene expression in ZR-75-1 cells and correlation between GCDFP-15 and estrogen as well as progesterone receptor expression in human breast cancer

Journal of Steroid Biochemistry ◽

10.1016/0022-4731(89)90115-5 ◽

1989 ◽

Vol 34 (1-6) ◽

pp. 397-402 ◽

Cited By ~ 15

Author(s):

Martine Dumont ◽

Sophie Dauvois ◽

Jacques Simard ◽

Teresa Garcia ◽

Beth Schachter ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Progesterone Receptor ◽

Human Breast Cancer ◽

Receptor Expression ◽

Progesterone Receptor Expression ◽

Human Breast

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In-vitro study of gonadotrophin signaling pathways in human granulosa cells in relation to progesterone receptor expression

Reproductive BioMedicine Online ◽

10.1016/j.rbmo.2017.06.011 ◽

2017 ◽

Vol 35 (4) ◽

pp. 363-371 ◽

Cited By ~ 12

Author(s):

Soledad Henríquez ◽

Paulina Kohen ◽

Alex Muñoz ◽

Ana Godoy ◽

Felipe Orge ◽

...

Keyword(s):

Progesterone Receptor ◽

Signaling Pathways ◽

Granulosa Cells ◽

In Vitro Study ◽

Receptor Expression ◽

Vitro Study ◽

Progesterone Receptor Expression ◽

Human Granulosa Cells

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Progesterone in vitro increases growth, motility and progesterone receptor expression in third stage larvae of Toxocara canis

Experimental Parasitology ◽

10.1016/j.exppara.2019.01.001 ◽

2019 ◽

Vol 198 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

L.E. Chávez-Güitrón ◽

J. Morales-Montor ◽

K.E. Nava-Castro ◽

H. Ramírez-Álvarez ◽

N.A. Moreno-Mendoza ◽

...

Keyword(s):

Progesterone Receptor ◽

Toxocara Canis ◽

Receptor Expression ◽

Progesterone Receptor Expression ◽

Third Stage

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Effects of testosterone and 2-hydroxyflutamide on progesterone receptor expression in porcine ovarian follicles in vitro

Reproductive Biology ◽

10.1016/j.repbio.2012.10.006 ◽

2012 ◽

Vol 12 (4) ◽

pp. 333-340 ◽

Cited By ~ 6

Author(s):

Malgorzata Duda ◽

Malgorzata Durlej-Grzesiak ◽

Zbigniew Tabarowski ◽

Maria Slomczynska

Keyword(s):

Progesterone Receptor ◽

Ovarian Follicles ◽

Receptor Expression ◽

Progesterone Receptor Expression

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238ESTROGEN RECEPTOR ALPHA AND PROGESTERONE RECEPTOR EXPRESSION FROM REPRODUCTIVE TISSUE AND IN VITRO PRODUCED EMBRYOS OF THE DOMESTIC CAT

Reproduction Fertility and Development ◽

10.1071/rdv16n1ab238 ◽

2004 ◽

Vol 16 (2) ◽

pp. 240 ◽

Cited By ~ 1

Author(s):

M.W. Latino ◽

T.C. Chiang ◽

C.E. Pope ◽

M.C. Gomez ◽

A.M. Giraldo ◽

...

Keyword(s):

Gene Expression ◽

Progesterone Receptor ◽

Real Time ◽

Real Time Pcr ◽

Female Reproductive Tract ◽

Receptor Expression ◽

Domestic Cat ◽

Reproductive Tissue ◽

Receptor Alpha

The in vitro production of cat embryos has been reported by several laboratories, and kittens have been born after transfer of embryos derived by IVF, ICSI, and NT. However, evidence accumulating in other species indicates that in vitro-derived embryos exhibit altered gene expression of developmentally important genes. Because the domestic cat genome is not well defined, the lack of primer sequence information poses a challenge for gene expression profiling. Estrogen, in addition to its essential role in the development and function of the female reproductive tract, is important for maturation of the oocyte. The aim of this preliminary study was to evaluate the expression profile of estrogen receptor alpha (ERα) and progesterone receptor (PR) in domestic cat female reproductive tissue and in vitro-produced (IVP) embryos. Embryos were produced in vitro as described by Gomez et al. (2003 Theriogenology 60, 239–251). mRNA was isolated from pools (n=35–50) of IVP domestic cat embryos at the morula and blastocyst stages (Days 7 and 8) using a modified protocol of the PolyAT Tract System (Promega, Madison, WI, USA). Uterus and ovaries were collected from hystorectomized cats and total RNA was isolated from these estrogen-targeted organs (RNeasy standard protocol, QIAGEN, Valencia, CA, USA). All RNA was reverse-transcribed and subjected to real-time PCR to evaluate expression of ERα and PR. The housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), was used as a control for expression in all tissues. Using primers designed to amplify human sequences or multispecies primers, we successfully amplified all three genes from as little as ∼100ng of ovarian or uterine mRNA. We were also able to detect ERα and GAPDH from pools of IVP embryos. Transcripts were cloned and confirmed by sequencing to be homologous to known sequences of the respective genes in a variety of species, including human, mouse, and pig. A 234-bp transcript of ERα (accession #AY349164;; GenBank) corresponding to exons 5 through 7 of the human ERα gene (hormone-binding domain) was identified along with a 110-bp sequence of PR and 98-bp sequence of GAPDH. To our knowledge, this is the first description of ERα or PR cloning in the domestic cat. In summary, we have demonstrated that highly sensitive real-time PCR is effective for the assessment of gene expression in cat ovarian and uterine tissue, as well as in embryos, the latter, of which, are known to have a low transcript copy number. Furthermore, these experiments provide the basis for future studies on the effect of exogenous estrogen on gene expression in cat IVM oocytes and IVP embryos.

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ÉTUDE IN VITRO DU MÉTABOLISME DES HYDRATES DE CARBONE DANS LE LEUCOCYTE CIRCULANT DU COBAYE TRAITÉ À LA CORTISONE

Acta Endocrinologica ◽

10.1530/acta.0.0510193 ◽

1966 ◽

Vol 51 (2) ◽

pp. 193-202

Author(s):

J. A. Antonioli ◽

A. Vannotti

Keyword(s):

Glucose Concentration ◽

Intramuscular Injection ◽

Acute Inflammation ◽

Glycogen Content ◽

Glycogen Synthesis ◽

Lactate Production ◽

Glucose Consumption ◽

List Type ◽

Hydrates De Carbone

ABSTRACT 1. The metabolism of suspensions of circulating leucocytes has been studied after intramuscular injection of a dose of 50 mg/kg of a corticosteroid (cortisone acetate). The suspensions were incubated under aerobic conditions in the presence of a glucose concentration of 5.6 mm. Glucose consumption, lactate production, and variations in intracellular glycogen concentration were measured. After the administration of the corticosteroid, the anabolic processes of granulocyte metabolism were reversibly stimulated. Glucose consumption and lactate production increased 12 hours after the injection, but tended to normalize after 24 hours. The glycogen content of the granulocytes was enhanced, and glycogen synthesis during the course of the incubation was greatly stimulated. The action of the administered corticosteroid is more prolonged in females than in males. The injection of the corticosteroid caused metabolic modifications which resemble in their modulations and in their chronological development those found in circulating granulocytes of guinea-pigs suffering from sterile peritonitis. These results suggest, therefore, that, in the case of acute inflammation, the glucocorticosteroids may play an important role in the regulation of the metabolism of the blood leucocytes.

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Anaerobic Glycolysis in Normal Human Erythrocytes Incubated in Vitro with Sodium Salicylate

Clinical Science ◽

10.1042/cs0490375 ◽

1975 ◽

Vol 49 (5) ◽

pp. 375-384

Author(s):

N. Worathumrong ◽

A. J. Grimes

Keyword(s):

Sodium Salicylate ◽

Lactate Production ◽

Glucose Consumption ◽

Human Erythrocytes ◽

Anaerobic Glycolysis ◽

Initial Stimulus ◽

Sodium Efflux ◽

Normal Human ◽

Concentration Changes

1. Some effects of sodium salicylate upon anaerobic glycolysis have been studied in normal human erythrocytes incubated for up to 6 h at 37°C in autologous sera. 2. Both glucose consumption and lactate production were stimulated by concentrations of salicylate up to 60 mmol/l but at the highest concentration used (90 mmol/l) an initial stimulus was followed by inhibition of glycolysis. 3. Losses occurred of adenosine 5′-triphosphate (ATP), adenosine 5′-diphosphate (ADP) and adenosine 5′-phosphate (AMP) at higher concentrations of salicylate and there was a concomitant increase of inorganic phosphate. 4. Other phosphate esters underwent concentration changes at higher concentrations of salicylate that reflected inadequate concentrations of ATP for glycolysis. 5. The rates of sodium efflux from, and potassium influx into, erythrocytes were unaffected by the presence of salicylate at concentrations sufficient to stimulate glycolysis.

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