9 TIMED ARTIFICIAL INSEMINATION IN BLOCKS: A NEW ALTERNATIVE TO IMPROVE FERTILITY IN BEEF COWS

2015 ◽  
Vol 27 (1) ◽  
pp. 97
Author(s):  
L. F. M. Pfeifer ◽  
N. A. Castro ◽  
L. G. B. Siqueira ◽  
K. R. Lagos ◽  
A. Bagon ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Meta Analysis ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Beef Cows ◽  
Ovulation Rate ◽  
Control Group ◽  
Block Group ◽  
Preovulatory Follicle ◽  
Timed Artificial Insemination

The objective of this study was to evaluate whether timed artificial insemination (TAI) according to the diameter of the dominant preovulatory follicle (POF) would improve pregnancy rates in beef cows. In Experiment 1, a retrospective meta-analysis of 5 oestradiol- or gonadotropin-releasing hormone-based TAI experiments from 2011 to 2014 was performed to evaluate the interval from luteolysis to ovulation. In these experiments, crossbred cows (Gyr × Holstein; n = 60) were examined by ultrasonography at 12-h intervals from progesterone-releasing device (CIDR) removal to ovulation. A linear regression model was used to predict the effect of POF diameter on the time of ovulation. Cows with a larger POF at the time of AI ovulated earlier than cows with smaller POF (y = –8.25x + 115.22; R2 = 0.93; P < 0.0001). In Experiment 2, lactating Nelore cows (Bos indicus; n = 59) on random days of the oestrous cycle were given 2 mg of oestradiol benzoate IM and a CIDR device (Day 0) to synchronize follicular waves. The CIDR were removed, and cows were given 500 μg of d-Cloprostenol (prostaglandin F2α) IM, 1 mg of oestradiol cypionate IM, and 300 IU of eCG IM on Day 8. On the morning of Day 10 (07:00 a.m.), the diameter of the POF was assessed by ultrasonography, and cows were randomised into Control (n = 29) and Block (n = 30) groups. 1) Control-group cows were TAI 48 h after CIDR removal (08:00 a.m. on Day 10), and Block-group cows were inseminated at 4 time points according to the diameter of the POF: B0 (POF ≥15 mm, TAI at 08:00 a.m. on Day 10, n = 6), B1 (POF 13 to 14 mm, TAI at 02:00 p.m. on Day 10, n = 8), B2 (POF 11 to 12 mm, TAI at 08:00 a.m. on Day 11, n = 11), and B3 (POF ≤ 10 mm, TAI at 02:00 p.m. on Day 11, n = 5). Pregnancy status was assessed 30 days post-AI by ultrasonography. No differences were detected in the diameter of the POF 48 h after CIDR removal (P = 0.77) or ovulation rate (P = 0.65; combined ovulation rate 52/59, 88%) between Control and Block groups. Block group had a higher pregnancy rate than Control (22/30, 73% v. 13/29, 45%, respectively; P = 0.02). In conclusion, our results document that interval from luteolysis to ovulation depends on the size of preovulatory follicle, timing of AI in relation to ovulation time is critical, and AI time according to the diameter of the POF can be an effective tool to improve fertility of cows in TAI protocols.

scholarly journals Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

2019 ◽  
Vol 6 (2) ◽  
Author(s):  
Mehmet Akkose ◽  
Cigdem Cebi Sen ◽  
Adnan Kirmit ◽  
Omer Korkmaz ◽  
Murat Onur Yazlik ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Fetal Death ◽  
Prostaglandin F2α ◽  
Control Group ◽  
Estrus Synchronization ◽  
Serum Progesterone ◽  
Death Rates ◽  
Dairy Heifers ◽  
Multiple Sampling ◽  
Timed Artificial Insemination

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations were assessed in Holstein heifers 28 days post-insemination, following estrus synchronization with either a double prostaglandin (PG) injection or the ovsynch protocol. Animals between 15 and 20 months of age, with a 3-3.5 BCS, were randomly assigned to three groups: Heifers in the control group (n = 70) were monitored for estrus behaviour for 21 days and inseminated when detected in standing heat; Estrus of heifers in the Ovsynch group (n = 70)) was synchronized following the Ovsynch protocol, and timed artificial insemination was performed 16 h after the completion of treatment; Animals in the PG group (n = 70) received two prostaglandin-F2α injections 11 days apart, were monitored for estrus behaviour for 2-5 days following the last injection, and inseminated when in heat. Serum PAGs and progesterone concentrations were measured in pregnant heifers (control group n = 25; ovsynch group n = 22; PG group n = 21) on day 28 after artificial insemination (AI). Mean serum PAGs concentrations (OD: optical density) were similar between groups (control = 3.37 ± 0.148, Ovsynch = 3.21 ± 0.136, and PG = 3.34 ± 0.143; p > 0.05). However, serum progesterone concentrations were found to be lower in the ovsynch group (4.70 ± 0.17 ng/mL) when compared to the PG (5.34 ± 0.13 ng/mL) or the control (5.37 ± 0.08 ng/mL) groups (p < 0.001). There was no correlation between serum PAGs and progesterone levels at day 28 post-insemination, or between early pregnancy PAGs concentrations and fetal death rates. Further research, including multiple sampling time points throughout gestation, after estrus synchronization protocols is warranted, to determine if there are associated temporal changes in PAGs and progesterone profiles that could impact reproductive parameters in dairy heifers.

scholarly journals Consequences of eight consecutive applications of equine chorionic gonadotropin on ovarian follicle development and ovulation in bos indicus cows

2016 ◽  
Vol 46 (11) ◽  
pp. 2018-2023
Author(s):  
Bhárbara Delboni Stuhr ◽  
Isaac Santos Gil ◽  
Tracy Ferreira Lacerda ◽  
Mauricio Gomes Favoreto ◽  
Bárbara Loureiro
Keyword(s):  
Chorionic Gonadotropin ◽  
Ovarian Follicle ◽  
Bos Indicus ◽  
Antral Follicle ◽  
Beef Cows ◽  
Ovulation Rate ◽  
Control Group ◽  
Estrus Synchronization ◽  
Antral Follicles ◽  
Equine Chorionic Gonadotropin

ABSTRACT: The equine chorionic gonadotropin (eCG) is a glycoprotein produced in mare endometrial calices. In bovine, it is used in estrus synchronization protocols. However, studies have shown that it is potentially immunogenic and its effect can decrease after repetitive use. This study aimed to evaluate antral follicle dynamics, corpus luteum (CL) and ovulation rate in bos indicus cows submitted to an estrus synchronization protocol in association with eCG for eight times consecutively. Ten cyclical, multiparous, and pasture raised beef cows were divided into two groups: control group (n=5) and eCG group (n=5). In 30 day interval, all animals were synchronized with the same estrogen/progesterone based protocol, totalizing 8 re-synchronizations. Cows in the treatment group received 300IU eCG 48 hours prior to the presumable ovulation. Ultrassound examinations were performed on Day 4 of the protocol (approximately 1.5 days after follicle recruitment) to count antral follicles, on Day 10, to count antral follicles and to measure size of the largest follicle and on Day 18 to measure the diameter of the CL. No difference (P>0.05) between follicular growth and size of the pre-ovulatory follicle was reported between groups. Cows treated with eCG had a larger (P<0.05) CL and increased (P<0.05) ovulation rate (18mm and 92%, respectively) when compared with control group (14.1mm and 80%, respectively). Furthermore, consecutive treatments did not affect CL nor ovulation rates. In conclusion, eCG treatment increased CL size and ovulation rate even after 8 consecutive treatments.

PSXII-20 Ovarian dynamics and fertility in beef cows administered a 5-day or 6-day CO-Synch protocol for timed artificial insemination

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 423-423
Author(s):  
Alexandria E Crist ◽  
Jessica Cristina Lemos Motta ◽  
Cameron Hayden ◽  
Benjamin Duran ◽  
Martin Mussard
Keyword(s):  
Mixed Models ◽  
Generalized Linear Mixed Models ◽  
Prostaglandin F2α ◽  
Beef Cows ◽  
Preovulatory Follicle ◽  
Follicle Diameter ◽  
Estrus Detection ◽  
Follicle Size ◽  
Timed Artificial Insemination ◽  
Ovarian Dynamics

Abstract The 5-day CO-Synch is an extensively used TAI protocol; however, it requires multiple administrations of prostaglandin F2α (PGF). The objective of the present study was to evaluate the effect of increasing progesterone (P4) device insertion from 5 to 6 days during a TAI protocol on ovarian dynamics and fertility in beef cows. Suckled beef cows (n = 867) at six locations were randomly assigned to either a 5-day or 6-day CO-Synch. Cows received an intravaginal P4 device (CIDR, Zoetis) and 100 µg of gonadorelin acetate (GnRH, Parnell) on d-9 (6-day) or d-8 (5-day). On d-3 CIDR’s were removed, an estrus detection patch applied (Estrotect, Rockway Inc.) and either one (6-day) or two (5-day) doses of PGF (500 µg of cloprostenol sodium, Estroplan, Parnell) was administered. On d0 (72 h after CIDR removal) all cows received 100 µg of GnRH, estrus expression was evaluated, and AI was performed. Pregnancy was determined by ultrasonography 34 days after TAI. Ovarian dynamics were evaluated in a subset of animals (n = 20) between d-3 and d7 to determine preovulatory follicle size, time of ovulation and corpus luteum (CL) size. Data were analyzed using generalized linear mixed models (SAS 9.4). Cows in the 6-day group tended (P = 0.09) to have a larger maximum follicle diameter (16.2±0.4mm) than cows in the 5-day group (15.0±0.6mm). In addition, CL volume on d7 was greater (P = 0.01) in the 6-day (4302±495mm3) than 5-day (2406±455mm3) group. There were, however, no differences (P = 0.15) in the interval between CIDR removal and ovulation. Cows assigned to the 6-day group had greater (P &lt; 0.05) estrus expression [56.1% (215/385) vs 41.5% (161/388)] and pregnancy rate [54.6% (236/438) vs 47.4% (207/435)] than cows in the 5-day group. In conclusion, extension of the period of P4 device insertion in a prolonged proestrus protocol increases preovulatory follicle size, estrous expression, and fertility to TAI.

Administering an additional prostaglandin F2α injection to Bos indicus beef cows during a treatment regimen for fixed-time artificial insemination

2020 ◽  
Vol 219 ◽  
pp. 106535
Author(s):  
Isabella M. Noronha ◽  
Reinaldo F. Cooke ◽  
Cícero F.G. Martins ◽  
Ramiro V. Oliveira Filho ◽  
Ky G. Pohler ◽  
...  
Keyword(s):  
Treatment Regimen ◽  
Artificial Insemination ◽  
Bos Indicus ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Beef Cows

scholarly journals Systemic changes caused by artificial insemination in beef cows (Bos indicus) and their impact on animal welfare

2021 ◽  
Vol 58 ◽  
pp. e183731
Author(s):  
Bruna Marcele Martins de Oliveira ◽  
Rubens Paes de Arruda ◽  
Milton Maturana Filho ◽  
Eduardo Harry Birgel Júnior ◽  
Daniela Becker Birgel ◽  
...  
Keyword(s):  
Animal Welfare ◽  
Artificial Insemination ◽  
Total Protein ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Beef Cows ◽  
Control Group ◽  
Management Procedures ◽  
Condition Score ◽  
The Moment

This study aimed to verify if the process of artificial insemination (AI) characterized here as animal immobilization, the passage of the semen applicator through the cervix, and deposition of the semen in the uterus, affected cows’ welfare. For this, 18 beef calved cows were selected and divided into two groups: inseminated cows (AIG, n = 9), and not inseminated cows, the control group (CG, n = 9). Body condition score, uterus, and ovary evaluation were performed. Later, both groups were submitted into an estrus synchronization protocol and only the AIG group was inseminated. Blood components of urea, creatinine, AST, GGT, CK, glucose, triglycerides, cholesterol, HDL, LDL, VLDL, NEFA, BHB, cortisol, estradiol, progesterone, albumin, and total protein were measured 30 h before AI, and 4, 24, 48 and 168 h after AI. Statistical differences were considered when P <0.05. No differences between AIG and CG were observed. On the other hand, when the moment of insemination was evaluated, differences were observed for urea, creatinine, AST, GGT, CK, glucose, triglycerides, NEFA, BHB, albumin, and total protein. There was an oscillation of metabolic profiles depending on the time and procedures to which animals were exposed, even though it could be inferred that the AI process was incapable of altering those metabolic components on animals that were inseminated. Still, we can affirm that artificial insemination cannot be categorized as a negative reproduction tool on animal welfare. However, the containment and management procedures for AI may alter the metabolic profile of cows, especially the increase of CK.

Sign in / Sign up

Export Citation Format

Share Document